An Alternative Fixative to Formalin Fixation for Molecular Applications: The RCL2®-CS100 Approach

Through the use of immunohistochemical techniques, human chorionic gonadotropin has been localized to syncytiotrophoblastic cells of immature placenta, hydatidiform mole, chorioadenoma destruens, and choriocarcinoma. No gonadotropin has been detected in cytotrophoblast. Evidence is discussed which suggests that syncytiotrophoblast is the cell of origin of human chorionic gonadotropin. The observation that formalin fixation did not alter the ability of human chorionic gonadotropin to react with its specific antibody permitted the study of formalin-fixed paraffin-embedded tissues stored in the tissue collection. In addition, the excellence of histologic preparations following formalin fixation facilitated cytologic identification.

Download Full-text

A Molecular Mechanism of Formalin Fixation and Antigen Retrieval

American Journal of Clinical Pathology ◽

10.1309/brn7ctx1e84nwwpl ◽

2004 ◽

Vol 121 (2) ◽

pp. 190-199 ◽

Cited By ~ 97

Author(s):

Seshi R. Sompuram ◽

Kodela Vani ◽

Elizabeth Messana ◽

Steven A. Bogen

Keyword(s):

Molecular Mechanism ◽

Formalin Fixation ◽

Antigen Retrieval

Download Full-text

Effects of Formalin Fixation, Paraffin Embedding, and Time of Storage on DNA Preservation in Brain Tissue: A BrainNet Europe Study

Brain Pathology ◽

10.1111/j.1750-3639.2007.00073.x ◽

2007 ◽

Vol 17 (3) ◽

pp. 297-303 ◽

Cited By ~ 90

Author(s):

Isidre Ferrer ◽

Judith Armstrong ◽

Sabina Capellari ◽

Piero Parchi ◽

Thomas Arzberger ◽

...

Keyword(s):

Brain Tissue ◽

Formalin Fixation ◽

Dna Preservation ◽

Paraffin Embedding

Download Full-text

Quantitative Ex Vivo MRI Changes due to Progressive Formalin Fixation in Whole Human Brain Specimens: Longitudinal Characterization of Diffusion, Relaxometry, and Myelin Water Fraction Measurements at 3T

Frontiers in Medicine ◽

10.3389/fmed.2018.00031 ◽

2018 ◽

Vol 5 ◽

Cited By ~ 18

Author(s):

Anwar S. Shatil ◽

Md Nasir Uddin ◽

Kant M. Matsuda ◽

Chase R. Figley

Keyword(s):

Human Brain ◽

Ex Vivo ◽

Formalin Fixation ◽

Water Fraction ◽

Myelin Water Fraction ◽

Mri Changes

Download Full-text

An Investigation of the Histochemical Technique for the Localization of Acid Phosphomonoesterase

Journal of Cell Science ◽

10.1242/jcs.s3-91.15.315 ◽

1950 ◽

Vol s3-91 (15) ◽

pp. 315-330

Author(s):

FRANCES MACDONALD

Keyword(s):

Enzyme Activity ◽

Acid Phosphatase ◽

Standard Technique ◽

Formalin Fixation ◽

Histochemical Technique ◽

Acid Phosphomonoesterase

1. A method is described for assessing the depth of ‘staining’ obtained with the acid phosphatase technique and a detailed scheme is given of the standard technique used. 2. It is concluded that the technique specifically demonstrates the activity of acid phosphatase, since ‘staining’ is abolished in the absence of substrate, in heated sections, and in the presence of fluoride. 3. An investigation has been carried out to determine the extent to which the reaction is affected by altering various stages in the technique. 4. The effect of formalin fixation on the reaction has been investigated. 5. It has been shown that sites in the rabbit medulla having an affinity for the reagents used in the technique differ from the sites at which a precipitate is deposited as a result of enzyme activity. 6. Evidence is presented suggesting that the technique may not demonstrate the true physiological localization of the enzyme. 7. It is suggested that the technique may be of value as a neurohistological method.

Download Full-text