The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair

2012 ◽  
pp. 79-90 ◽  
Author(s):  
Günter Speit ◽  
Andreas Rothfuss
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Dna Damage And Repair ◽  
Genotoxicity Test

DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace

2006 ◽  
Vol 167 (2) ◽  
pp. 131-141 ◽  
Author(s):  
Maria Enrica Fracasso ◽  
Denise Doria ◽  
Paola Franceschetti ◽  
Luigi Perbellini ◽  
Luciano Romeo
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
White Collar ◽  
Repair Capacity ◽  
Dna Damage And Repair

DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

2010 ◽  
Vol 51 (1) ◽  
pp. 79-86 ◽  
Author(s):  
G. Brozovic ◽  
N. Orsolic ◽  
R. Rozgaj ◽  
V. Kasuba ◽  
F. Knezevic ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Micronucleus Test ◽  
Alkaline Comet Assay ◽  
Dna Damage And Repair ◽  
Swiss Albino Mice ◽  
Albino Mice

Heterogeneity in Radiation-Induced DNA Damage and Repair in Tumor and Normal Cells Measured Using the “Comet” Assay

2012 ◽  
Vol 178 (2) ◽  
pp. AV35-AV42 ◽  
Author(s):  
Peggy L. Olive ◽  
Judit P. Banáth ◽  
Ralph E. Durand
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Normal Cells ◽  
Dna Damage And Repair ◽  
Radiation Induced

scholarly journals Recent Physical Activity in Relation to DNA Damage and Repair Using the Comet Assay

2014 ◽  
Vol 11 (4) ◽  
pp. 770-776 ◽  
Author(s):  
Stephanie Whisnant Cash ◽  
Shirley A.A. Beresford ◽  
Thomas L. Vaughan ◽  
Patrick J. Heagerty ◽  
Leslie Bernstein ◽  
...  
Keyword(s):  
Physical Activity ◽  
Dna Damage ◽  
Dna Repair ◽  
Comet Assay ◽  
High Intensity ◽  
Total Activity ◽  
Washington State ◽  
Dna Damage And Repair ◽  
Activity Intensity ◽  
Very High

Background:Limited evidence suggests that very high-intensity exercise is positively associated with DNA damage but moderate exercise may be associated with DNA repair.Methods:Participants were 220 healthy, Washington State 50- to 76-year-olds in the validity/biomarker substudy of the VITamins And Lifestyle (VITAL) cohort, who provided blood samples and completed questionnaires assessing recent physical activity and demographic and health factors. Measures included nested activity subsets: total activity, moderate- plus high-intensity activity, and high-intensity activity. DNA damage (n = 122) and repair (n = 99) were measured using the comet assay. Multivariate linear regression was used to estimate regression coefficients and associated 95% confidence intervals (CIs) for relationships between MET-hours per week of activity and each DNA outcome (damage, and 15- and 60-minute repair capacities).Results:DNA damage was not associated with any measure of activity. However, 60-minute DNA repair was positively associated with both total activity (β = 0.21, 95% CI: 0.0057–0.412; P = .044) and high-intensity activity (β = 0.31, 95% CI: 0.20–0.60; P = .036), adjusting for age, sex, BMI, and current multivitamin use.Conclusions:This study is the first to assess broad ranges of activity intensity levels related to DNA damage and repair. Physical activity was unrelated to DNA damage but was associated with increased repair.

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