Direct Methods for The Computation of a Nonsimple Turning Point Corresponding to a Cusp

Author(s):  
Dirk Roose ◽  
Renaat Caluwaerts
Keyword(s):  
Author(s):  
James F. Hainfeld

Lipids are an important class of molecules, being found in membranes, HDL, LDL, and other natural structures, serving essential roles in structure and with varied functions such as compartmentalization and transport. Synthetic liposomes are also widely used as delivery and release vehicles for drugs, cosmetics, and other chemicals; soap is made from lipids. Lipids may form bilayer or multilammellar vesicles, micelles, sheets, tubes, and other structures. Lipid molecules may be linked to proteins, carbohydrates, or other moieties. EM study of this essential ingredient of life has lagged, due to lack of direct methods to visualize lipids without extensive alteration. OsO4 reacts with double bonds in membrane phospholipids, forming crossbridges. This has been the method of choice to both fix and stain membranes, thus far. An earlier work described the use of tungstate clusters (W11) attached to lipid moieties to form lipid structures and lipid probes.


Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.


2003 ◽  
Vol 3 (2) ◽  
pp. 197-202
Author(s):  
Kristjana Kristinsdóttir
Keyword(s):  

Author(s):  
Danilo Ballotta ◽  
Tim Pfeiffer-Gerschel ◽  
Alexis Goosdeel
Keyword(s):  

1999 ◽  
pp. 95
Author(s):  
Editorial board Of the Journal

In the 10th issue of the Bulletin “Ukrainian Religious Studies” in the rubric “Scientific Reports and Announcements” there are in particular the following papers: “Religious Studies and Theology” by A.Kolodny, “Activity of the Orthodox Mission in Ukraine on the Turning Point of the XIX-XXth Centuries” by G.Nadtoka, “Religion in the Spiritual Heritage of V.Lypinsky” by L.Kondratyk, “Church as a Factor of the Self-identification of the Nation in the Cultural and Civilization Environment” by O.Nedavnya, “The Problems of Development of The Social Teaching of the Catholicism” by V.Sergyiko, “The God-Thunder Perun in the Pagan World-outlook of the Ancient Rus’” by N.Fatyushyna and other papers


2012 ◽  
Vol 22 (5) ◽  
pp. 154-154
Author(s):  
Jennifer H. Martinick
Keyword(s):  

2017 ◽  
Vol 26 (4) ◽  
pp. 391-410 ◽  
Author(s):  
David McCrone

The Brexit referendum in 2016 was a major turning-point in British and Scottish politics, reflected in a majority for Leave in England, but for Remain in Scotland. This article uses the British and Scottish Social Surveys for 2016 to explain Scottish-English differences, and finds that there were broad similarities in terms of social and demographic characteristics, and in terms of social values (‘authoritarians’ voting for Leave). Being ‘English’, however, was much more significant than being ‘Scottish’ in accounting for Brexit vote. The association between Brexit vote and constitutional preferences, notably voting intention in a future Scottish Independence Referendum, is far less clear-cut. Brexit promises to be a political game-changer, but in ways which are complex and unpredictable.


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