Steroid Profiling: Analytical Perspectives

2018 ◽  
pp. 27-40
Author(s):  
Gary M. Woodward ◽  
Gill Rumsby
Keyword(s):  
Steroid Profiling

Prediction of affective disorders in adult men on the basis of steroid profiling

2015 ◽  
Author(s):  
Martin Hill ◽  
Daniela Ripova ◽  
Pavel Mohr ◽  
Marta Velikova ◽  
Marie Bicikova ◽  
...  
Keyword(s):  
Affective Disorders ◽  
Adult Men ◽  
Steroid Profiling

Impact of Clomiphene Citrate on the Steroid Profile in Dysmetabolic Men with Low Testosterone Levels

2021 ◽  
Vol 53 (08) ◽  
pp. 520-528
Author(s):  
Carla Pelusi ◽  
Flamina Fanelli ◽  
Margherita Baccini ◽  
Giovanni De Pergola ◽  
Vincenzo Triggiani ◽  
...  
Keyword(s):  
Clomiphene Citrate ◽  
Serum Levels ◽  
Male Hypogonadism ◽  
Hormonal Changes ◽  
Double Blind ◽  
Adrenal Steroidogenesis ◽  
Steroid Profiling ◽  
Corticosteroid Binding Globulin ◽  
Before And After ◽  
17Β Estradiol

AbstractClomiphene citrate (CC) in male hypogonadism increases testosterone (T) and estrogen levels by stimulating pituitary gonadotropin release. Our group confirmed these hormonal changes in a randomized, cross-over, double-blind trial of CC versus placebo in addition to metformin, conducted in 21 obese dysmetabolic men with low T levels. However, we hypothesize that based on its mechanism of action, CC may directly or indirectly affect adrenal steroidogenesis. The aim of this sub-study was to better understand the changes in steroid levels and metabolism induced by CC treatment. We assessed 17α-hydroxypregnelone (17αOH-P5), dehydroepiandrosterone (DHEA), progesterone (P4), 17α-hydroxyprogesterone (17αOH-P4), androstenedione (A), T, dihydrotestosterone (DHT), estrone (E1), 17β-estradiol (E2), 11-deoxycortisol (11 S), cortisol (F), and cortisone (E) by LC-MS/MS, and corticosteroid binding globulin (CBG) by ELISA, before and after each treatment. In addition, free-F and steroid product/precursor ratios were calculated. We observed a significant change in serum levels induced by CC compared with placebo for 17αOH-P4, DHT, T, E2, E1, F, E, and CBG, but not free-F. In addition, compared to placebo, CC induced higher 17αOH-P4/P4, E2/E1, 17αOH-P4/17αOH-P5, A/17αOH-P4, T/A, E1/A, F/11 S, and F/E ratios. Therefore, besides the CC stimulating effect on testis steroidogenesis, our study showed increased F, E, but not free-F, levels, indicating changes in steroid metabolism rather than adrenal secretion stimulation. The steroid profiling also revealed the CC stimulation of the Δ5 rather than the Δ4 pathway, thus indicating considerable testicular involvement in the increased androgen secretion.

scholarly journals Urinary steroid profiling in diagnostic evaluation of an unusual adrenal mass

2019 ◽  
Vol 2019 ◽  
Author(s):  
N F Lenders ◽  
J R Greenfield
Keyword(s):  
Sensitivity And Specificity ◽  
Serum Biochemistry ◽  
Diagnostic Evaluation ◽  
Gas Chromatography Mass Spectrometry ◽  
List Type ◽  
Steroid Profiling ◽  
Rare Tumours ◽  
Adrenal Masses ◽  
Urinary Steroid

Summary Adrenal oncocytomas are rare tumours, with only approximately 160 cases reported in the literature. We report the use of urinary steroid profiling as part of their diagnostic evaluation and prognostication. A 45-year-old woman presented with clinical features of hyperandrogenism. Serum biochemistry confirmed androgen excess and computed tomography (CT) demonstrated a 3.2 cm adrenal tumour with density 39 HU pre-contrast. Urine steroid profiling showed elevated tetrahydro-11 deoxycortisol (THS), which is associated with adrenal malignancy. Laparoscopic adrenalectomy was performed, and histopathology diagnosed adrenal oncocytoma. Serum and urinary biochemistry resolved post-operatively and remained normal at 1-year follow-up. Learning points: Differential diagnosis of adrenal masses is challenging. Current techniques for differentiating between tumour types lack sensitivity and specificity. 24-h urinary steroid profiling is a useful tool for reflecting steroid output from adrenal glands. Gas chromatography-mass spectrometry (GC-MS) of urinary steroid metabolites has sensitivity and specificity of 90% for diagnosing adrenocortical carcinoma. Adrenal oncocytoma are rare tumours. Differentiating between benign and malignant types is difficult. Data guiding prognostication and management are sparse.

scholarly journals Is steroid profiling using LC-MS/MS useful in the diagnostic work-up of primary aldosteronism?

2016 ◽  
Author(s):  
Tracy A. Williams ◽  
Jacques W.M. Lenders ◽  
Mirko Peitzsch ◽  
Tanja Dekkers ◽  
Anna S. Dietz ◽  
...  
Keyword(s):  
Primary Aldosteronism ◽  
Steroid Profiling ◽  
Diagnostic Work ◽  
Work Up ◽  
Diagnostic Work Up

An LC–MS/MS method for steroid profiling during adrenal venous sampling for investigation of primary aldosteronism

2015 ◽  
Vol 145 ◽  
pp. 75-84 ◽  
Author(s):  
Mirko Peitzsch ◽  
Tanja Dekkers ◽  
Matthias Haase ◽  
Fred C.G.J. Sweep ◽  
Ivo Quack ◽  
...  
Keyword(s):  
Primary Aldosteronism ◽  
Adrenal Venous Sampling ◽  
Venous Sampling ◽  
Steroid Profiling

scholarly journals Comparison of Strategies for the Determination of Sterol Sulfates via GC-MS Leading to a Novel Deconjugation-Derivatization Protocol

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2353 ◽  
Author(s):  
Julia Junker ◽  
Isabelle Chong ◽  
Frits Kamp ◽  
Harald Steiner ◽  
Martin Giera ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemical Cleavage ◽  
Advantages And Disadvantages ◽  
Chromatography Mass Spectrometry ◽  
Steroid Profiling ◽  
Health And Disease ◽  
Indispensable Tool

Sulfoconjugates of sterols play important roles as neurosteroids, neurotransmitters, and ion channel ligands in health and disease. In most cases, sterol conjugate analysis is performed with liquid chromatography-mass spectrometry. This is a valuable tool for routine analytics with the advantage of direct sterol sulfates analysis without previous cleavage and/or derivatization. The complementary technique gas chromatography-mass spectrometry (GC-MS) is a preeminent discovery tool in the field of sterolomics, but the analysis of sterol sulfates is hampered by mandatory deconjugation and derivatization. Despite the difficulties in sample workup, GC-MS is an indispensable tool for untargeted analysis and steroid profiling. There are no general sample preparation protocols for sterol sulfate analysis using GC-MS. In this study we present a reinvestigation and evaluation of different deconjugation and derivatization procedures with a set of representative sterol sulfates. The advantages and disadvantages of trimethylsilyl (TMS), methyloxime-trimethylsilyl (MO-TMS), and trifluoroacetyl (TFA) derivatives were examined. Different published procedures of sterol sulfate deconjugation, including enzymatic and chemical cleavage, were reinvestigated and examined for diverse sterol sulfates. Finally, we present a new protocol for the chemical cleavage of sterol sulfates, allowing for simultaneous deconjugation and derivatization, simplifying GC-MS based sterol sulfate analysis.

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