Mitochondria Apoptosis Pathway

2011 ◽  
pp. 2331-2331
Keyword(s):  
Apoptosis Pathway ◽  
Mitochondria Apoptosis Pathway

scholarly journals Exosome-mediated apoptosis pathway during WSSV infection in crustacean mud crab

2020 ◽  
Author(s):  
Yi Gong ◽  
Tongtong Kong ◽  
Xin Ren ◽  
Jiao Chen ◽  
Shanmeng Lin ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Viral Infection ◽  
Dna Fragmentation ◽  
Protein Complex ◽  
Cellular Response ◽  
Mud Crab ◽  
Apoptosis Pathway ◽  
Exosomal Mirnas ◽  
Apoptosis Inducing Factor ◽  
Mitochondria Apoptosis Pathway

AbstractMicroRNAs are regulatory molecules that can be packaged into exosomes to modulate recipient’s cellular response, while their role during viral infection is beginning to be appreciated. However, the involvement of exosomal miRNAs during immunoregulation in invertebrates has not been addressed. Here, we found that exosomes released from WSSV-injected mud crabs could suppress viral invasion by inducing apoptosis of hemocytes. Besides, miR-137 and miR-7847 were found to be less packaged in mud crab exosomes during viral infection, with both miR-137 and miR-7847 shown to be negative apoptosis regulators by targeting the apoptosis-inducing factor (AIF). Moreover, our data revealed that AIF did not only translocate to the nucleus to induce DNA fragmentation, but could also competitively bind to HSP70 to disintegrate the HSP70-Bax (Bcl-2-associated X protein) complex, which eventually activated the mitochondria apoptosis pathway via free Bax. Therefore, our findings provides a novel mechanism underlying the crosstalk between exosomal miRNAs and apoptosis pathway in innate immunity in invertebrates.

scholarly journals The Effects of ATO on Mitochondria Apoptosis Pathway Genes Expression in APL Cell Line

2016 ◽  
Vol 1 (2) ◽  
pp. 37-42
Author(s):  
Maede Sharifizadeh ◽  
Kimia Davarian ◽  
Mehrdad Hashemi ◽  
Majid Momeni ◽  
Kamran Ali Moghdam ◽  
...  
Keyword(s):  
Cell Line ◽  
Arsenic Trioxide ◽  
Caspase 3 ◽  
Single Agent ◽  
Cdna Synthesis ◽  
Apoptosis Pathway ◽  
Genes Expression ◽  
Analytical Work ◽  
Mitochondria Pathway ◽  
Mitochondria Apoptosis Pathway

Purpose and Background: Acute promyelocystic leukemia is the most malignant acute leukemia that leads to death in few weeks. It constitutes 10-15% of acute myelocystic leukemia. Arsenic trioxide, as a single agent factor, is known as the best treatment for acute promyelocystic leukemia, which mainly functions by inducing apoptosis. However, there is no clear image of the mechanism through which apoptosis is induced and how the genes expression is deeply affected. Thus, the present study is an attempt to examine the effect of the agent on expression of the genes dealing with the cancer. Methodology: The study was carried out as an analytical work. To find out about the mechanisms effective on inducing apoptosis, cell line NB4 were cultured with 0.5µM, 1 µM, and 2 µM arsenic trioxide and their RNA was extracted after 12hrs, 24hrs, 28hrs, and 72hrs. Following cDNA synthesis, apoptosis genes expression at mitochondria pathway including caspase 3, Mcl-1, and Bcl-2 were examined using Real-Time PCR. The data was analyzed using t-test and variance analysis in Excel.Findings: It was found that arsenic caused apoptosis was featured with decrease of mRNA expression of Bcl-2 anti-apoptotic. However, expression of caspase 3 and Mcl-1 genes remained unchanged after culturing by arsenic. Conclusion: The results showed that changes in Bcl-2 gene expression can be considered as a mechanism of apoptosis caused by arsenic, while caspase 3 and Mcl-1 genes had no effect on the mechanism.

Induction of the Mitochondria Apoptosis Pathway by Phytohemagglutinin Erythroagglutinating in Human Lung Cancer Cells

2010 ◽  
Vol 18 (3) ◽  
pp. 848-856 ◽  
Author(s):  
Wei-Ting Kuo ◽  
Yung-Jen Ho ◽  
Shyh-Ming Kuo ◽  
Feng-Huei Lin ◽  
Fuu-Jen Tsai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Apoptosis Pathway ◽  
Human Lung Cancer Cells ◽  
Mitochondria Apoptosis Pathway

Mitochondria apoptosis pathway synergistically activated by hierarchical targeted nanoparticles co-delivering siRNA and lonidamine

Biomaterials ◽  
2015 ◽  
Vol 61 ◽  
pp. 178-189 ◽  
Author(s):  
Bing-Feng Zhang ◽  
Lei Xing ◽  
Peng-Fei Cui ◽  
Feng-Zhen Wang ◽  
Rong-Lin Xie ◽  
...  
Keyword(s):  
Apoptosis Pathway ◽  
Targeted Nanoparticles ◽  
Mitochondria Apoptosis Pathway
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