Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry

2000 ◽  
pp. 29-52
Author(s):  
L. R. Castellanos-Serra ◽  
E. Hardy ◽  
W. Proenza ◽  
V. Huerta ◽  
L. J. Gonzalez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proteome Analysis ◽  
Negative Staining ◽  
Zinc Salts

Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts

2007 ◽  
pp. 152-157
Author(s):  
Lila Castellanos-Serra ◽  
Wilfredo Proenza ◽  
Vivian Huerta ◽  
Robert L. Moritz ◽  
Richard J. Simpson
Keyword(s):  
Proteome Analysis ◽  
Polyacrylamide Gel ◽  
Negative Staining ◽  
Zinc Salts

Proteome analysis of polyacrylamide gel-separated proteins visualized by reversible negative staining using imidazole-zinc salts

1999 ◽  
Vol 20 (4-5) ◽  
pp. 732-737 ◽  
Author(s):  
Lila Castellanos-Serra ◽  
Wilfredo Proenza ◽  
Vivian Huerta ◽  
Robert L. Moritz ◽  
Richard J. Simpson
Keyword(s):  
Proteome Analysis ◽  
Polyacrylamide Gel ◽  
Negative Staining ◽  
Zinc Salts

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

Proteome Analysis ofLactobacillus rhamnosusGG Using 2-D DIGE and Mass Spectrometry Shows Differential Protein Production in Laboratory and Industrial-Type Growth Media

2009 ◽  
Vol 8 (11) ◽  
pp. 4993-5007 ◽  
Author(s):  
Kerttu Koskenniemi ◽  
Johanna Koponen ◽  
Matti Kankainen ◽  
Kirsi Savijoki ◽  
Soile Tynkkynen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Production ◽  
Proteome Analysis ◽  
Growth Media ◽  
Differential Protein
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