Development of Monoclonal Antibodies for the Detection and Identification of Monilinia spp. Causing Brown Rot of Stone and Pome Fruit

1997 ◽  
pp. 391-393 ◽  
Author(s):  
J. N. Banks ◽  
R. H. Rizvi ◽  
C. R. Lane ◽  
K. J. D. Hughes ◽  
R. T. A. Cook
Keyword(s):  
Monoclonal Antibodies ◽  
Brown Rot ◽  
Pome Fruit ◽  
Detection And Identification

Monoclonal Antibodies and Human Autoantisera Used for the Detection and Identification of Nuclear Matrix Components

1985 ◽  
pp. 275-278
Author(s):  
R. VERHEIJEN ◽  
H.J.H. KUIJPERS ◽  
F.C.S. RAMAEKERS ◽  
G.P. VOOIJS ◽  
W.J. VAN VENROOIJ
Keyword(s):  
Monoclonal Antibodies ◽  
Nuclear Matrix ◽  
Detection And Identification ◽  
Matrix Components

Development of monoclonal antibodies for the rapid detection and identification of Salmonella enterica serovar Enteritidis in food sample using dot‐blot assays

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Chontichar Jinapon ◽  
Pradit Wangman ◽  
Chalinan Pengsuk ◽  
Parin Chaivisuthangkura ◽  
Paisarn Sithigorngul ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Food Sample ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Detection And Identification

Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR

2006 ◽  
Vol 133 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Mohamed Hassan ◽  
Arben Myrta ◽  
Jaroslav Polak
Keyword(s):  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pome Fruit ◽  
Detection And Identification

scholarly journals Preparation of Monoclonal Antibodies for Detection and Identification of Francisella tularensis

2006 ◽  
Vol 14 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Akitoyo Hotta ◽  
Akihiko Uda ◽  
Osamu Fujita ◽  
Kiyoshi Tanabayashi ◽  
Akio Yamada
Keyword(s):  
Monoclonal Antibodies ◽  
Francisella Tularensis ◽  
Bacterial Cells ◽  
Detection And Identification

ABSTRACT Monoclonal antibodies (MAbs) against Francisella tularensis were obtained. Three MAbs specifically reacted with F. tularensis, while four MAbs reacted with other members of the genus Francisella as well. Fluorescent isothiocyanate-conjugated MAbs unequivocally stained bacterial cells in specimens from experimentally infected mice. Two MAbs agglutinated F. tularensis antigen in the agglutination tests. These MAbs should improve methods for detection and identification of F. tularensis.

Monilinia fructigena (brown rot).

2021 ◽  
Author(s):  
Keyword(s):  
New Species ◽  
New Zealand ◽  
Brown Rot ◽  
Fruit Trees ◽  
The Other ◽  
Eastern Asia ◽  
Pome Fruit ◽  
Monilinia Fructigena ◽  
The Usa ◽  
A New Species

Abstract M. fructigena is one of several apothecial ascomycetes causing brown rot and blossom blight of stone fruit and pome fruit trees worldwide. It has a more restricted distribution than the other species, occurring in Europe and Asia, but not in North America. Reports of its occurrence in South America are likely to be errors in identification. Recent identification of a new species in Japan suggests that it may not be present there, as previously thought, and reports from other parts of eastern Asia may have to be re-examined. It is a quarantine pest for Canada, the USA, Australia and New Zealand. One unusual introduction to the USA was resolved by eradication (Batra, 1979; Ogawa and English, 1991). Introduction could occur through the importation of infected fruit as well as of tree material for propagation and breeding, from which it could spread readily by means of conidia carried by the wind or insects.

Immuno-scanning electron microscopic localization of extracellular wood-degrading enzymes within the fibrillar sheath of the brown-rot fungus Postia placenta

1992 ◽  
Vol 38 (9) ◽  
pp. 898-904 ◽  
Author(s):  
Frederick Green III ◽  
Carol A. Clausen ◽  
Michael J. Larsen ◽  
Terry L. Highley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Brown Rot ◽  
Postia Placenta ◽  
Degrading Enzymes ◽  
Brown Rot Fungus ◽  
Hyphal Sheath ◽  
Scanning Electron

Extracellular wood-degrading enzymes of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the β-1,4-xylanase (32 to 36 kDa) fraction of P. placenta. Postia placenta was grown from agar onto glass cover slips, immunolabeled with or without prior fixation, and examined by scanning electron microscopy. Enzymes were localized on the hyphal surface and on the clumped fibrillar elements mycofibrils of the hyphal sheath following fixation with glutaraldehyde. If fixation was omitted, labeling was diffuse and not localized on individual or clumped mycofibrils. We conclude that extracellular decay enzymes are weakly bound (noncovalently) to, but not identical with, the linear mycofibrillar elements of the hyphal sheath. The linear structural elements of the hyphal sheath may play an important role in transport and presentation of wood-degrading enzymes during the decay process. Key words: brown-rot fungi, enzymes, mycofibrils, hyphal sheath, immunolabeling, monoclonal antibodies, colloidal gold, scanning electron microscopy.

scholarly journals Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Miljan Vasić ◽  
Ivana Vico ◽  
Wayne M. Jurick ◽  
Nataša Duduk
Keyword(s):  
Pathogen Detection ◽  
Management Strategies ◽  
Brown Rot ◽  
Morphological Characterization ◽  
Apple Fruit ◽  
Postharvest Disease ◽  
Pome Fruit

Brown rot, caused by Monilinia spp., is an economically important pre- and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre- and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.

scholarly journals The use of monoclonal antibodies in autoimmunity treatment

2018 ◽  
pp. 150-154
Author(s):  
E.M. Merzlyak ◽  
D.S. Syrko ◽  
E.A. Musatkina ◽  
M.A. Israelson
Keyword(s):  
Monoclonal Antibodies ◽  
Adverse Effects ◽  
Medical Application ◽  
Therapeutic Agents ◽  
Mechanisms Of Action ◽  
T And B Cells ◽  
Detection And Identification ◽  
Cell Clones ◽  
Contemporary Medicine ◽  
Autoreactive Lymphocyte

Recently, monoclonal antibodies (MA) have gained popularity as therapeutic agents for the treatment of autoimmune disorders. These antibodies target proinflammatory cytokines, as well as T and B cells potentially involved in the pathogenesis of such conditions. In the present work we attempt to give a systematic description of available therapeutic MA, highlight their key mechanisms of action and pinpoint their adverse effects. We believe that MA that are capable of recognizing and eliminating pathogenic T- and B-cell clones hold the most promise for medical application as biologics. Detection and identification of autoreactive lymphocyte clones is one of the most serious challenges of contemporary medicine.

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