Isolation of Oxygen Evolving PSII Particles and PSII Reaction Centres from the Filamentous Cyanobacterium Spirulina Platensis

1995 ◽  
pp. 695-698
Author(s):  
Eva Šetlíková ◽  
Petr Budáč ◽  
Danuše Sofrová
Keyword(s):  
Spirulina Platensis ◽  
Filamentous Cyanobacterium ◽  
Reaction Centres ◽  
Oxygen Evolving

Kinetic response of photosystem II photochemistry in the cyanobacterium Spirulina platensis to high salinity is characterized by two distinct phases

1999 ◽  
Vol 26 (3) ◽  
pp. 283 ◽  
Author(s):  
Congming Lu ◽  
Giuseppe Torzillo ◽  
Avigad Vonshak
Keyword(s):  
Electron Transport ◽  
Quantum Yield ◽  
Photosystem Ii ◽  
Spirulina Platensis ◽  
High Salinity ◽  
Photochemical Quenching ◽  
Second Phase ◽  
Ps Ii ◽  
Reaction Centres ◽  
Two Phases

The kinetic response of photosystem II (PS II) photochemistry in Spirulina platensis(Norstedt M2 ) to high salinity (0.75 M NaCl) was found to consist of two phases. The first phase, which was independent of light, was characterized by a rapid decrease (15–50%) in the maximal efficiency of PS II photochemistry (Fv /Fm), the efficiency of excitation energy capture by open PS II reaction centres (Fv′/Fm′), photochemical quenching (qp) and the quantum yield of PS II electron transport (Φ PS II) in the first 15 min, followed by a recovery up to about 80–92% of their initial levels within the next 2 h. The second phase took place after 4 h, in which further decline in above parameters occurred. Such a decline occurred only when the cells were incubated in the light, reaching levels as low as 45–70% of their initial levels after 12 h. At the same time, non-photochemical quenching (qN) and Q B -non-reducing PS II reaction centres increased significantly in the first 15 min and then recovered to the initial level during the first phase but increased again in the light in the second phase. The changes in the probability of electron transfer beyond QA (ψo) and the yield of electron transport beyond QA (φ Eo), the absorption flux (ABS/RC) and the trapping flux (TRo /RC) per PS II reaction centre also displayed two different phases. The causes responsible for the decreased quantum yield of PS II electron transport during the two phases are discussed.

Temperature Dependence of the O2-Oscillation Pattern in the Filamentous Cyanobacterium Oscillatoria chalybea and in Chlorella kessleri

1996 ◽  
Vol 51 (11-12) ◽  
pp. 823-832 ◽  
Author(s):  
K Burda ◽  
P He ◽  
K. P Bader ◽  
G. H Schmid
Keyword(s):  
Transition Temperature ◽  
Temperature Dependence ◽  
Oxygen Evolution ◽  
Filamentous Cyanobacterium ◽  
State Distribution ◽  
Oscillation Pattern ◽  
Chlorella Kessleri ◽  
Temperature Range ◽  
Oscillatoria Chalybea ◽  
Oxygen Evolving

Abstract Five characteristic discontinuities of the pattern of oxygen evolution have been detected for the filamentous cyanobacterium Oscillatoria chalybea in the temperature range of 0°C to 30°C. The temperatures at which these discontinuities occur are: ≈ 5°C, ≈ 11°C, ≈ 15°C, ≈ 21°C and ≈ 25°C. The calculated initial 5-S state distribution, the miss parameter and the fraction of the fast transition S3 → S0+ O2 are affected. The discontinuities are observed at the same transition temperature also for Chlorella kessleri hence are not specific for the cyanobacterium. Based on these studies it is concluded that the not vanishing oxygen signal under the first flash of a flash train in Oscillatoria cannot have its origin in interactions between oxygen-evolving complexes. A decrease of temperature should slow down the expected charge exchanges, improve the oscillations, thus reduce or lower the first two oxygen amplitudes of the oscillatoria pattern. Lowering of the temperautres improves the oscillations but does not lower the first O2 signal of the pattern.

The response of the filamentous cyanobacterium Spirulina platensis to salt stress

1988 ◽  
Vol 150 (5) ◽  
pp. 417-420 ◽  
Author(s):  
Avigad Vonshak ◽  
Rachel Guy ◽  
Micha Guy
Keyword(s):  
Salt Stress ◽  
Spirulina Platensis ◽  
Filamentous Cyanobacterium

Inhibition of quantum yield of PS II electron transport in Spirulina platensis by osmotic stress may be explained mainly by an increase in the proportion of the QB-non-reducing PS II reaction centres

1998 ◽  
Vol 25 (6) ◽  
pp. 689 ◽  
Author(s):  
Congming Lu ◽  
Jianhua Zhang ◽  
Avigad Vonshak
Keyword(s):  
Electron Transport ◽  
Quantum Yield ◽  
Osmotic Stress ◽  
Spirulina Platensis ◽  
Fluorescence Induction ◽  
Photochemical Quenching ◽  
Ps Ii ◽  
Reaction Centres ◽  
Induction Kinetics ◽  
Fluorescence Induction Kinetics

Modulated chlorophyll fluorescence and fluorescence induction kinetics were used to evaluate the PS II photochemistry in Spirulina platensis exposed to osmotic stress (0–0.8 M mannitol). Osmotic stress decreased the efficiency of excitation energy capture by open PS II reaction centres (Fv′/Fm′) and more significantly, decreased photochemical quenching (qP). Osmotic stress also decreased the maximal efficiency of PS II photochemistry (Fv/Fm). There was no significant change in non-photochemical quenching (qN), indicating that the decreased Fv′/Fm′ was not due to an increase in qN. Analyses of the fast fluorescence induction kinetics indicated that osmotic stress caused a significant increase in the proportion of the QB-non-reducing PS II reaction centres. Based on the results in this study, we suggest that a substantial increase in the proportion of the QB-non-reducing PS II reaction centres may be responsible for the decrease in qP and Fv′/Fm′, of which both resulted in the decrease in the quantum yield of PS II electron transport (ΦPSII ).

Independently Acting Oxygen-Forming Complexes of Photosystem II in Cyanobacteria A Study in the Filamentous Cyanobacterium Oscillatoria chalybea

1997 ◽  
Vol 52 (3-4) ◽  
pp. 169-174 ◽  
Author(s):  
S. Spiegel ◽  
K. Burda ◽  
K. P. Bader ◽  
G. H. Schmid
Keyword(s):  
Photosystem Ii ◽  
Cross Sections ◽  
Dark Adaptation ◽  
Filamentous Cyanobacterium ◽  
Damped Oscillations ◽  
Synechococcus Leopoliensis ◽  
Evolution Pattern ◽  
Oscillatoria Chalybea ◽  
The Individual ◽  
Oxygen Evolving

Abstract It has been postulated that the oxygen-evolving centers of photosystem II do not operate independently in the cyanobacterium Synechococcus leopoliensis in contrast to those of the chlorophyte Chlorella vulgaris and the diatom Phaeodactylum tricornutum (Mauzerall and Dubinsky (1993), Biochim. Biophys. Acta 1183, 123-129). Dependence would mean the existence of charge transfer among adjacent units and would be manifested by different saturation curves for the individual flashes of a sequence (different cross-sections), stronger damped oscillations and oxygen formation under the first flash, independently of the length of dark adaptation. We show in the present publication that in the filamentous cyanobacterium Oscillatoria chalybea the O2-evolution pattern which shows an O2-signal under the first flash (despite dark adaptation) can be explained within the heterogeneous Kok-model, assuming a non-standard initial S-state distribution (Bader, Thibault and Schmid (1983), Z. Naturforsch. 38c. 778-792).

scholarly journals Growth, Pigment and Protein Production of Spirulina platensis under different Ca(NO3)2 concentrations

2020 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Muhammad Fakhri ◽  
Prive Widya Antika ◽  
Arning Wilujeng Ekawati ◽  
Nasrullah Bai Arifin
Keyword(s):  
Protein Content ◽  
Spirulina Platensis ◽  
Large Scale ◽  
Protein Production ◽  
Nitrate Concentration ◽  
Calcium Nitrate ◽  
Fish Feed ◽  
Filamentous Cyanobacterium ◽  
Significant Difference ◽  
Nitrate Concentrations

Spirulina platensis is a filamentous cyanobacterium that has been commerically used for fish feed and human food supplement. Low-cost production of Spirulina is needed when considering large-scale culture especially for industrial purposes. The aim of this study was to explain the effect of calcium nitrate (Ca(NO3)2) on growth, biomass, pigment, and protein production of S. platensis and to determine the best calcium nitrate concentration for Spirulina production.The microalgae was cultured at four calcium nitrate concentrations (1, 1.5, 2.0 and 2.5 g/L) with salinity of 15 ppt, constant light intensity of 4,000 lux and photoperiod of 24:0 light:dark cycles for 4 days. The results showed that different calcium nitrate concentrations remarkably affected the growth, biomass production, pigment and protein content of S. platensis (p<0.05). The highest specific growth rate of 0.721 day-1 and biomass concentration of 1.512 g/Lwere achieved at calcium nitrate concentration of 2.5 g/L. Moreover, the algae had the highest chlorophyll-a, carotenoid and protein content at 2.5 g L-1. Increasing calcium nitrate concentration from 1 to 2.5 g/L led to an increase in biomass, pigment and protein production of S. platensis. However, there was no significant difference between 2 and 2.5 g L-1 calcium nitrate concentrations. We suggest that 2-2.5 g/L Ca(NO3)2 concentration can be used profitably for S. platensis production.

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