Hybrid Breeding by Means of Incompatibility in Lolium Multiflorum

Hybrid breeding of watermelon has led to the formation of high-yielding varieties. The objectives of the research were to determine the performance of hybrid watermelon of the Indonesian Tropical Fruit Research Institute (ITFRI) collection and to evaluate the superiority of each hybrid. The research has been conducted at Sumani Experimental Garden, ITFRI, Solok, West Sumatera, from April to July 2016. The material used consisted of 19 cross combinations. The data from this descriptive study were presented in the form of averages and pictures. The results showed that the hybrids that were tested showed diversity in size, fruit skin color, flesh color, skin thickness and fruit sweetness. Hybrids with three excellent characters, i.e. medium fruit weight, high total soluble solids (TSS) value, and moderate rind thickness, were not yet obtained. Hybrids that had the superiority in term of sweet taste and shelf life were BT1 × SGP, BT4 × BT4P, BT5 × BT4, BT6 × SGP and SGP × BT6. Keywords: hybrid, performance, total soluble solids, watermelon ABSTRAK Pemuliaan hibrida pada semangka mengarah kepada pembentukan varietas berdaya hasil tinggi. Penelitian bertujuan untuk mengetahui keragaan hibrida semangka koleksi Balitbu Tropika dan untuk mengevaluasi keunggulan masing-masing hibrida Penelitian dilaksanakan di Kebun Percobaan Sumani, Balitbu Tropika, Solok, Sumatera Barat, dari bulan April sampai dengan Juli 2016. Materi yang digunakan terdiri dari 19 kombinasi persilangan. Data hasil penelitian deskriptif ini ditampilkan dalam bentuk rataan dan gambar. Hasil penelitian menunjukkan bahwa hibrida yang diuji memiliki keragaman dalam ukuran, warna kulit buah, warna daging buah, ketebalan kulit buah dan kemanisan. Belum ditemukan hibrida yang memiliki tiga karakter unggul, yang terdiri dari bobot buah sedang, total padatan terlarut (total soluble solid) tinggi, dan kulit buah agak tebal. Hibrida yang memiliki keunggulan berupa rasa manis dan tahan simpan adalah BT1 × SGP, BT4 × BT4P, BT5 × BT4, BT6 × SGP dan SGP × BT6. Kata kunci: hibrida, keragaan, semangka, total padatan terlarut

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Effects of Italian ryegrass (Lolium multiflorum Lam.) shoots and roots on inhibition of weed growth

Research on Crops ◽

10.31830/2348-7542.2019.064 ◽

2019 ◽

Vol 20 (2) ◽

Keyword(s):

Lolium Multiflorum ◽

Italian Ryegrass ◽

Weed Growth

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Enhancement of Rural Income and Nutrition by Cultivating Pigeonpea Hybrids

Journal of AgriSearch ◽

10.21921/jas.v7i04.19387 ◽

2020 ◽

Vol 7 (04) ◽

Author(s):

K B SAXENA ◽

A K CHOUDHARY ◽

RAFAT K SULTANA

Keyword(s):

Cajanus Cajan ◽

Family Income ◽

National Level ◽

Hybrid Breeding ◽

High Food ◽

Rural Income ◽

Breeding Technology ◽

On Farm ◽

Food Value

Pigeonpea [Cajanus cajan (L.) Millsp.] is a favourite crop of rain-fed farmers due to its high food value, drought tolerance and various soil improving properties. The productivity enhancement of this crop has been a long-term goal at the national level but with a little success. In this context, the advent of hybrid breeding technology with over 30% on-farm yield advantages has provided a much-needed breakthrough. The hybrids in pigeonpea were bred using a stable CMS system and natural out-crossing. It is believed that the adoption of locally adapted hybrids would contribute significantly towards both family income and nutrition.

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Growth and Phenology of Vulpia Myuros in Comparison with Apera Spica-Venti, Alopecurus Myosuroides and Lolium Multiflorum in Monoculture and in Winter Wheat

Plants ◽

10.3390/plants9111495 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1495

Author(s):

Muhammad Javaid Akhter ◽

Bo Melander ◽

Solvejg Kopp Mathiassen ◽

Rodrigo Labouriau ◽

Svend Vendelbo Nielsen ◽

...

Keyword(s):

Winter Wheat ◽

Weed Management ◽

Lolium Multiflorum ◽

Management Strategies ◽

Integrated Weed Management ◽

Growth Stages ◽

Neighborhood Design ◽

Alopecurus Myosuroides ◽

Winter Cereals ◽

Arable Weed

Vulpia myuros has become an increasing weed problem in winter cereals in Northern Europe. However, the information about V. myuros and its behavior as an arable weed is limited. Field and greenhouse experiments were conducted in 2017/18 and 2018/19, at the Department of Agroecology in Flakkebjerg, Denmark to investigate the emergence, phenological development and growth characteristics of V. myuros in monoculture and in mixture with winter wheat, in comparison to Apera spica-venti, Alopecurus myosuroides and Lolium multiflorum. V. myuros emerged earlier than A. myosuroides and A. spica-venti but later than L. multiflorum. Significant differences in phenological development were recorded among the species. Overall phenology of V. myuros was more similar to that of L. multiflorum than to A. myosuroides and A. spica-venti. V. myuros started seed shedding earlier than A. spica-venti and L. multiflorum but later than A. myosuroides. V. myuros was more sensitive to winter wheat competition in terms of biomass production and fecundity than the other species. Using a target-neighborhood design, responses of V. myuros and A. spica-venti to the increasing density of winter wheat were quantified. At early growth stages “BBCH 26–29”, V. myuros was suppressed less than A. spica-venti by winter wheat, while opposite responses were seen at later growth stages “BBCH 39–47” and “BBCH 81–90”. No significant differences in fecundity characteristics were observed between the two species in response to increasing winter wheat density. The information on the behavior of V. myuros gathered by the current study can support the development of effective integrated weed management strategies for V. myuros.

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A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03920-0 ◽

2021 ◽

Author(s):

Junping Yu ◽

Guolong Zhao ◽

Wei Li ◽

Ying Zhang ◽

Peng Wang ◽

...

Keyword(s):

Transcription Factor ◽

Glycine Max ◽

Male Sterility ◽

Bulked Segregant Analysis ◽

Soybean Protein ◽

Anther Development ◽

Hybrid Breeding ◽

Myb Transcription Factor ◽

Male Sterile ◽

R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

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Quantitative trait loci analysis of nitrate-nitrogen content in Italian ryegrass (Lolium multiflorum Lam.)

Euphytica ◽

10.1007/s10681-020-02737-0 ◽

2021 ◽

Vol 217 (1) ◽

Author(s):

Wenqing Tan ◽

Di Zhang ◽

Nana Yuyama ◽

Jun Chen ◽

Shinichi Sugita ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Nitrogen Content ◽

Quantitative Trait ◽

Nitrate Nitrogen ◽

Lolium Multiflorum ◽

Italian Ryegrass ◽

Trait Loci

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Genome-Wide Association and Prediction of Male and Female Floral Hybrid Potential Traits in Elite Spring Bread Wheat Genotypes

Plants ◽

10.3390/plants10050895 ◽

2021 ◽

Vol 10 (5) ◽

pp. 895

Author(s):

Samira El Hanafi ◽

Souad Cherkaoui ◽

Zakaria Kehel ◽

Ayed Al-Abdallat ◽

Wuletaw Tadesse

Keyword(s):

Genomic Selection ◽

Bread Wheat ◽

Genome Wide Association ◽

Hybrid Breeding ◽

Floral Traits ◽

Phenotypic Variance ◽

Spring Bread Wheat ◽

Genome Wide ◽

Anther Length ◽

Anther Extrusion

Hybrid wheat breeding is one of the most promising technologies for further sustainable yield increases. However, the cleistogamous nature of wheat displays a major bottleneck for a successful hybrid breeding program. Thus, an optimized breeding strategy by developing appropriate parental lines with favorable floral trait combinations is the best way to enhance the outcrossing ability. This study, therefore, aimed to dissect the genetic basis of various floral traits using genome-wide association study (GWAS) and to assess the potential of genome-wide prediction (GP) for anther extrusion (AE), visual anther extrusion (VAE), pollen mass (PM), pollen shedding (PSH), pollen viability (PV), anther length (AL), openness of the flower (OPF), duration of floret opening (DFO) and stigma length. To this end, we employed 196 ICARDA spring bread wheat lines evaluated for three years and genotyped with 10,477 polymorphic SNP. In total, 70 significant markers were identified associated to the various assessed traits at FDR ≤ 0.05 contributing a minor to large proportion of the phenotypic variance (8–26.9%), affecting the traits either positively or negatively. GWAS revealed multi-marker-based associations among AE, VAE, PM, OPF and DFO, most likely linked markers, suggesting a potential genomic region controlling the genetic association of these complex traits. Of these markers, Kukri_rep_c103359_233 and wsnp_Ex_rep_c107911_91350930 deserve particular attention. The consistently significant markers with large effect could be useful for marker-assisted selection. Genomic selection revealed medium to high prediction accuracy ranging between 52% and 92% for the assessed traits with the least and maximum value observed for stigma length and visual anther extrusion, respectively. This indicates the feasibility to implement genomic selection to predict the performance of hybrid floral traits with high reliability.

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