Lateral Flow Nucleic Acid Biosensors

2016 ◽  
pp. 245-273
Author(s):  
Wentao Xu
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

scholarly journals Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane

2020 ◽  
Vol 38 (3) ◽  
pp. 199
Author(s):  
Narendra Yoga Hendarta ◽  
Abu Tholib Aman ◽  
Asmarani Kusumawati ◽  
Tri Wibawa
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Gc Content ◽  
Lateral Flow ◽  
Hybridization Signal ◽  
Nitrocellulose Membrane ◽  
High Concentration ◽  
Cross Hybridization ◽  
High Stringency ◽  
Stringency Condition

Lateral flow assay (LFD) based nucleic acid lateral flow (NALF)  method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based on nucleic acid hybridizationis  more economical then immunochromatography assay which use antibody-antigen recognition. Cross hybridization has issued while used to differentiate organism with high GC content and high homology as high similarity genome. Some techniques has applied to give high stringency condition avoid cross hybridization reaction but need more procedure to apply. We found glycerol applied to buffer assay could reduce cross hybridization on nitrocellulose membrane. The study used 2 kinds of high stringency buffer as PBS and SSC bases and high concentration of ssDNA amplicon as sample. Without glycerol ingredient gave cross hybridization signal on test line. But used glycerol could reduce those even omitted with PBS based buffer assay. Beside those, glycerol could significantly increased hybridization signal in SSC based buffer assay (p<0.05).

Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters

2015 ◽  
Vol 864 ◽  
pp. 48-54 ◽  
Author(s):  
Eleni A. Sapountzi ◽  
Sotirios S. Tragoulias ◽  
Despina P. Kalogianni ◽  
Penelope C. Ioannou ◽  
Theodore K. Christopoulos
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Lateral Flow ◽  
Nucleic Acid Analysis ◽  
Flow Devices

Development of a dry-reagent-based nucleic acid-sensing platform by coupling thermostabilised LATE-PCR assay to an oligonucleotide-modified lateral flow biosensor

2015 ◽  
Vol 118 ◽  
pp. 99-105 ◽  
Author(s):  
Geik Yong Ang ◽  
Choo Yee Yu ◽  
Kok Gan Chan ◽  
Kirnpal Kaur Banga Singh ◽  
Yean Chan Yean
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Pcr Assay ◽  
Sensing Platform

Development of a nucleic acid lateral flow strip for rapid, visual detection of Nosema bombycis in silkworm eggs

2019 ◽  
Vol 164 ◽  
pp. 59-65 ◽  
Author(s):  
Zhangshuai He ◽  
Qi Ni ◽  
Yue Song ◽  
Rui Wang ◽  
Yunlin Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Nosema Bombycis

scholarly journals Plasmodium Detection and Differentiation by Direct-on-Blood PCR Nucleic Acid Lateral Flow Immunoassay

2018 ◽  
Vol 20 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Johanna M. Roth ◽  
Laura de Bes ◽  
Patrick Sawa ◽  
George Omweri ◽  
Victor Osoti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC

2017 ◽  
Vol 200 ◽  
pp. 101-106 ◽  
Author(s):  
Constanze Seidel ◽  
Sonja Peters ◽  
Erik Eschbach ◽  
Andrea T. Feßler ◽  
Boris Oberheitmann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Resistance Genes ◽  
Rapid Detection ◽  
Methicillin Resistance ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay
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