Biogenic Silver Nanoparticles from Trametes ljubarskyi (White Rot Fungus): Efficient and Effective Anticandidal Activity

There is growing interest in the development of non-toxic, natural wood preservation agents to replace conventional chemicals. In this paper, the antifungal activities of silver nanoparticles, chitosan oligomers, and propolis ethanolic extract were evaluated against white-rot fungus Trametes versicolor (L.) Lloyd, with a view to protecting Populus spp. wood. In order to create a more realistic in-service type environment, the biocidal products were assessed according to EN:113 European standard, instead of using routine in vitro antimicrobial susceptibility testing methods. Wood blocks were impregnated with the aforementioned antifungal agents by the vacuum-pressure method in an autoclave, and their biodeterioration was monitored over 16 weeks. The results showed that treatments based on silver nanoparticles, at concentrations ranging from 5 to 20 ppm, presented high antifungal activity, protecting the wood from fungal attack over time, with weight losses in the range of 8.49% to 8.94% after 16 weeks, versus 24.79% weight loss in the control (untreated) samples. This was confirmed by SEM and optical microscopy images, which showed a noticeably higher cell wall degradation in control samples than in samples treated with silver nanoparticles. On the other hand, the efficacy of the treatments based on chitosan oligomers and propolis gradually decreased over time, which would be a limiting factor for their application as wood preservatives. The nanometal-based approach is thus posed as the preferred choice for the industrial treatment of poplar wood aimed at wood-based engineering products (plywood, laminated veneer lumber, cross-laminated timber, etc.).

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Biomimetics of silver nanoparticles by white rot fungus, Phaenerochaete chrysosporium

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2006.07.014 ◽

2006 ◽

Vol 53 (1) ◽

pp. 55-59 ◽

Cited By ~ 223

Author(s):

Nadanathangam Vigneshwaran ◽

Arati A. Kathe ◽

P.V. Varadarajan ◽

Rajan P. Nachane ◽

R.H. Balasubramanya

Keyword(s):

Silver Nanoparticles ◽

White Rot ◽

White Rot Fungus

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Synthesis of silver nanoparticles using white-rot fungus Anamorphous Bjerkandera sp. R1: influence of silver nitrate concentration and fungus growth time

Scientific Reports ◽

10.1038/s41598-021-82514-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jerónimo Osorio-Echavarría ◽

Juliana Osorio-Echavarría ◽

Claudia Patricia Ossa-Orozco ◽

Natalia Andrea Gómez-Vanegas

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Silver Nitrate ◽

Biological Properties ◽

White Rot ◽

White Rot Fungus ◽

Growth Time ◽

Efficient Production ◽

Enzymatic System ◽

Vis Spectroscopy

AbstractCurrently, silver nanoparticles (AgNPs) constitute an interesting field of study in medicine, catalysis, optics, among others. For this reason, it has been necessary to develop new methodologies that allow a more efficient production of AgNPs with better antimicrobial and biological properties. In this research growth time effects Anamorphous Bjerkandera sp. R1 and the silver nitrate (AgNO3) concentration over AgNPs synthesis were studied. Through the protocol used in this work, it was found that the action of the capping proteins on the surface of the mycelium played a determining role in the reduction of the Ag+ ion to Ag0 nanoparticles producing a particle size that oscillated between 10 and 100 nm. The progress of the reaction was monitored using visible UV–Vis spectroscopy and the synthesized AgNPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared radiation (FTIR) spectroscopy. The best synthetic properties were found at 1 mM of AgNO3 concentration, growth time of 8 days, and reaction time of 144 h. Nanometals obtention from microorganisms could be considered as a new method of synthesis, due to reducing abilities of metal ions through its enzymatic system and represents low-cost synthesis that reduces the generation of harmful toxic wastes.

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Effect of Neem Oil (Azadirachtin) Against White Rot Fungus (Polyporous Versicolor) in Wood Plastic Particle Board

International Journal of Scientific Research ◽

10.15373/22778179/jan2014/177 ◽

2012 ◽

Vol 3 (1) ◽

pp. 20-21

Author(s):

A.Sangeetha A.Sangeetha ◽

◽

K.Thanigai K.Thanigai ◽

Narasimhamurthy Narasimhamurthy ◽

S.K.Nath S.K.Nath

Keyword(s):

White Rot ◽

White Rot Fungus ◽

Particle Board ◽

Neem Oil ◽

Plastic Particle

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Effects of White Rot Fungus Physisporinus sp., Isolated in Japan, on Styrene-butadiene Rubber

NIPPON GOMU KYOKAISHI ◽

10.2324/gomu.93.289 ◽

2020 ◽

Vol 93 (9) ◽

pp. 289-292

Author(s):

Yumi SHIMIZU ◽

Shuma SATHO ◽

Taro NAKAJIMA ◽

Hiroaki KOUZAI ◽

Kiminori SHIMIZU

Keyword(s):

White Rot ◽

White Rot Fungus ◽

Styrene Butadiene Rubber ◽

Butadiene Rubber ◽

Styrene Butadiene

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Synergistic Methodology Based on Ion Exchange and Biodegradation Mechanisms Applied for Metal Complex Dye Removal from Waste Waters

Revista de Chimie ◽

10.37358/rc.18.1.6041 ◽

2018 ◽

Vol 69 (1) ◽

pp. 38-44

Author(s):

Nicoleta Mirela Marin ◽

Olga Tiron ◽

Luoana Florentina Pascu ◽

Mihaela Costache ◽

Mihai Nita Lazar ◽

...

Keyword(s):

Ion Exchange ◽

Synergistic Effects ◽

Freundlich Isotherm ◽

White Rot ◽

White Rot Fungus ◽

Basic Anion Exchange Resin ◽

Km Value ◽

Points Of View ◽

Basic Anion

This study investigates the synergistic effects of ion exchange and biodegradation methods to remove the Acid Blue 193 also called Gryfalan Navy Blue RL (GNB) dye from wastewater. Ion exchange studies were performed using a strongly basic anion exchange resin Amberlite IRA 400. The equilibrium was characterized by a kinetic and thermodynamic points of view, establishing that the sorption of the GNB dye was subject to the Freundlich isotherm model with R2 = 0.8710. Experimental results showed that the activated resin can removed up to 93.4% when the concentration of dye solution is 5.62�10-2 mM. The biodegradation of the GNB was induced by laccase, an enzyme isolated from white-rot fungus. It was also analyzed the role of pH and dye concentration on GNB biodegradation, so 5�10-2 mM dye had a maximum discoloration efficiency of 82.9% at pH of 4. The laccase showed a very fast and robust activity reaching in a few minutes a Km value of 2.2�10-1mM. In addition, increasing the GNB concentration up to 8�10-1 mM did not triggered a substrat inhibition effect on the laccase activity. Overall, in this study we proposed a mixt physicochemical and biological approach to enhance the GNB removal and biodegradability from the wastewaters and subsequently the environment.

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The Onset of Lignin-Modifying Enzymes, Decrease of AOX and Color Removal by White-Rot Fungi Grown on Bleach Plant Effluents

Water Science & Technology ◽

10.2166/wst.1991.0475 ◽

1991 ◽

Vol 24 (3-4) ◽

pp. 189-198 ◽

Cited By ~ 37

Author(s):

V. P. Lankinen ◽

M. M. Inkeröinen ◽

J. Pellinen ◽

A. I. Hatakka

Keyword(s):

Lignin Peroxidase ◽

Active Form ◽

White Rot Fungi ◽

Pulp Mill ◽

Color Removal ◽

Effluent Treatment ◽

White Rot ◽

White Rot Fungus ◽

Lignin Peroxidases ◽

Pulp Mill Effluents

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.

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