Spatially and temporally regulated expression of the MADS-box gene AGL2 in wild-type and mutant arabidopsis flowers

1994 ◽  
Vol 26 (2) ◽  
pp. 581-595 ◽  
Author(s):  
Catherine A. Flanagan ◽  
Hong Ma
Keyword(s):  
Mads Box ◽  
Wild Type ◽  
Regulated Expression ◽  
Mads Box Gene

scholarly journals The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development

Development ◽  
1998 ◽  
Vol 125 (8) ◽  
pp. 1509-1517 ◽  
Author(s):  
Q. Gu ◽  
C. Ferrandiz ◽  
M.F. Yanofsky ◽  
R. Martienssen
Keyword(s):  
Fruit Development ◽  
Cell Types ◽  
Enhancer Trap ◽  
Mads Box ◽  
Wild Type ◽  
Primary Defect ◽  
Hybridization Data ◽  
Enhancer Trap Line ◽  
Cell Layers ◽  
Mads Box Gene

Fruit morphogenesis is a process unique to flowering plants, and yet little is known about its developmental control. Following fertilization, fruits typically undergo a dramatic enlargement that is accompanied by differentiation of numerous distinct cell types. We have identified a mutation in Arabidopsis called fruitfull (ful-1), which abolishes elongation of the silique after fertilization. The ful-1 mutation is caused by the insertion of a DsE transposable enhancer trap element into the 5′ untranslated leader of the AGL8 MADS-box gene. beta-glucuronidase (GUS) reporter gene expression in the enhancer trap line is observed specifically in all cell layers of the valve tissue, but not in the replum, the septum or the seeds, and faithfully mimics RNA in situ hybridization data reported previously. The lack of coordinated growth of the fruit tissues leads to crowded seeds, a failure of dehiscence and, frequently, the premature rupture of the carpel valves. The primary defect of ful-1 fruits is within the valves, whose cells fail to elongate and differentiate. Stomata, which are frequent along the epidermis of wild-type valves, are completely eliminated in the ful mutant valves. In addition to the effect on fruit development, ful cauline leaves are broader than those of wild type and show a reduction in the number of internal cell layers. These data suggest that AGL8/FUL regulates the transcription of genes required for cellular differentiation during fruit and leaf development.

VvMADS9, a class B MADS-box gene involved in grapevine flowering, shows different expression patterns in mutants with abnormal petal and stamen structures

2006 ◽  
Vol 33 (9) ◽  
pp. 877 ◽  
Author(s):  
Lekha Sreekantan ◽  
Laurent Torregrosa ◽  
Lucie Fernandez ◽  
Mark R. Thomas
Keyword(s):  
Expression Pattern ◽  
Expression Patterns ◽  
In Situ Hybridisation ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Mads Box ◽  
Wild Type ◽  
Organ Identity ◽  
Class B ◽  
Mads Box Gene

VvMADS9, a MADS-box gene, from grapevine (Vitis vinifera L.) cultivar Cabernet Sauvignon has been isolated and its expression pattern studied in wild type Cabernet Sauvignon, Mourvèdre, and Bouchalès cultivars and mutants of the latter two genotypes showing abnormal petal / stamen structures. Sequence analysis showed that VvMADS9 was highly similar to PISTILLATA (PI), the class B gene that specifies the identity of petals and stamens in Arabidopsis. The temporal expression pattern of VvMADS9 studied through real-time PCR revealed that its expression was specific to flower development. The low levels of expression in the Mourvèdre mutant and the skewed expression pattern in the Bouchalès mutant as compared to their wild type counterparts suggested that VvMADS9 is involved in normal formation of petals and stamens. Through in situ hybridisation, expression of VvMADS9 was detected in stamens and weak expression on the basal regions of the petals. This suggested a possible role for VvMADS9 in specifying stamen and petal organ identity in grapevine similar to Class B genes in other species. All evidence thus pointed to the conclusion that VvMADS9 is an orthologue of PISTILLATA in grapevine.

scholarly journals Utilizing MIKC-type MADS-box protein SOC1 for yield potential enhancement in maize

2021 ◽  
Author(s):  
Guo-qing Song ◽  
Xue Han ◽  
John T. Ryner ◽  
Addie Thompson ◽  
Kan Wang
Keyword(s):  
Transgenic Plants ◽  
Plant Height ◽  
No Reduction ◽  
Planting Density ◽  
Yield Potential ◽  
Grain Production ◽  
Mads Box ◽  
Young Leaves ◽  
Mads Box Gene ◽  
Transcriptome Comparison

Abstract Key message Overexpression of Zea mays SOC gene promotes flowering, reduces plant height, and leads to no reduction in grain production per plant, suggesting enhanced yield potential, at least, through increasing planting density. Abstract MIKC-type MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) is an integrator conserved in the plant flowering pathway. In this study, the maize SOC1 (ZmSOC1) gene was cloned and overexpressed in transgenic maize Hi-II genotype. The T0 plants were backcrossed with nontransgenic inbred B73 to produce first generation backcross (BC1) seeds. Phenotyping of both transgenic and null segregant (NT) BC1 plants was conducted in three independent experiments. The BC1 transgenic plants showed new attributes such as increased vegetative growth, accelerated flowering time, reduced overall plant height, and increased grain weight. Second generation backcross (BC2) plants were evaluated in the field using two planting densities. Compared to BC2 NT plants, BC2 transgenic plants, were 12–18% shorter, flowered 5 days earlier, and showed no reduction in grain production per plant and an increase in fat, starch, and simple sugars in the grain. Transcriptome comparison in young leaves of 56-day-old BC1 plants revealed that the overexpressed ZmSOC1 resulted in 107 differentially expressed genes. The upregulated transcription factor DNA BINDING WITH ONE FINGER 5.4 (DOF5.4) was among the genes responsible for the reduced plant height. Modulating expression of SOC1 opens a new and effective approach to promote flowering and reduce plant height, which may have potential to enhance crop yield and improve grain quality.

Genome-wide identification and analysis of the MADS-box gene family and its potential role in fruit ripening in black raspberry (Rubus occidentalis L.)

2021 ◽  
pp. 1-15
Author(s):  
Yaqiong Wu ◽  
Chunhong Zhang ◽  
Wenlong Wu ◽  
Weilin Li ◽  
Lianfei Lyu
Keyword(s):  
Gene Family ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Expression Patterns ◽  
Type I ◽  
Black Raspberry ◽  
Mads Box ◽  
Mads Box Genes ◽  
Genome Wide ◽  
Mads Box Gene

BACKGROUND: Black raspberry is a vital fruit crop with a high antioxidant function. MADS-box genes play an important role in the regulation of fruit development in angiosperms. OBJECTIVE: To understand the regulatory role of the MADS-box family, a total of 80 MADS-box genes were identified and analyzed. METHODS: The MADS-box genes in the black raspberry genome were analyzed using bioinformatics methods. Through an analysis of the promoter elements, the possible functions of different members of the family were predicted. The spatiotemporal expression patterns of members of the MADS-box family during black raspberry fruit development and ripening were systematically analyzed. RESULTS: The genes were classified into type I (Mα: 33; Mβ: 6; Mγ: 10) and type II (MIKC *: 2; MIKCC: 29) genes. We also obtained a complete overview of the RoMADS-box gene family through phylogenetic, gene structure, conserved motif, and cis element analyses. The relative expression analysis showed different expression patterns, and most RoMADS-box genes were more highly expressed in fruit than in other tissues of black raspberry. CONCLUSIONS: This finding indicates that the MADS-box gene family is involved in the regulation of fruit ripening processes in black raspberry.

scholarly journals Molecular Characterization and Phylogenetic Analysis of MADS-Box Gene VroAGL11 Associated with Stenospermocarpic Seedlessness in Muscadine Grapes

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 232
Author(s):  
M Atikur Rahman ◽  
Subramani P Balasubramani ◽  
Sheikh M Basha
Keyword(s):  
Phylogenetic Analysis ◽  
Phosphorylation Site ◽  
Orthologous Gene ◽  
Mads Box ◽  
Vitis Species ◽  
Vitis Rotundifolia ◽  
Seed Content ◽  
Putative Phosphorylation Site ◽  
Mads Box Gene ◽  
Muscadine Grapes

Reduced expression of MADS-box gene AGAMOUS-LIKE11 (VviAGL11) is responsible for stenospermocarpic seedlessness in bunch grapes. This study is aimed to characterize the VviAGL11 orthologous gene (VroAGL11) in native muscadine grapes (Vitis rotundifolia) at the molecular level and analyze its divergence from other plants. The VroAGL11 transcripts were found in all muscadine cultivars tested and highly expressed in berries while barely detectable in leaves. RT-PCR and sequencing of predicted ORFs from diverse grape species showed that AGL11 transcripts were conservatively spliced. The encoded VroAGL11 protein contains highly conserved MADS-MEF2-like domain, MADS domain, K box, putative phosphorylation site and two sumoylation motifs. The muscadine VroAGL11 proteins are almost identical (99%) to that of seeded bunch cultivar, Chardonnay, except in one amino acid (A79G), but differs from mutant protein of seedless bunch grape, Sultanina, in two amino acids, R197L and T210A. Phylogenetic analysis showed that AGL11 gene of muscadine and other Vitis species formed a separate clade than that of other eudicots and monocots. Muscadine grape cultivar “Jane Bell” containing the highest percentage of seed content in berry (7.2% of berry weight) had the highest VroAGL11 expression, but almost none to nominal expression in seedless cultivars Fry Seedless (muscadine) and Reliance Seedless (bunch). These findings suggest that VroAGL11 gene controls the seed morphogenesis in muscadine grapes like in bunch grape and can be manipulated to induce stenospermocarpic seedlessness using gene editing technology.

A MADS‐box gene with similarity to FLC is induced by cold and correlated with epigenetic changes to control budbreak in kiwifruit

2021 ◽  
Author(s):  
Charlotte Voogd ◽  
Lara A Brian ◽  
Rongmei Wu ◽  
Tianchi Wang ◽  
Andrew C Allan ◽  
...  
Keyword(s):  
Epigenetic Changes ◽  
Mads Box ◽  
Mads Box Gene
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