Conditional inhibition of β-glucuronidase expression by antisense gene fragments in petunia protoplasts

1993 ◽  
Vol 23 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Pieter de Lange ◽  
Gert-Jan de Boer ◽  
Joseph N. M. Mol ◽  
Jan M. Kooter
Keyword(s):  
Antisense Gene ◽  
Petunia Protoplasts ◽  
Conditional Inhibition

scholarly journals Regulation of Activator/Dissociation Transposition by Replication and DNA Methylation

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1723-1733
Author(s):  
Francesca Ros ◽  
Reinhard Kunze
Keyword(s):  
Dna Methylation ◽  
Transposable Elements ◽  
Strong Evidence ◽  
Complementary Strand ◽  
Regulatory Effect ◽  
Ds Excision ◽  
Dna Strand ◽  
Petunia Protoplasts ◽  
Ac Transposase ◽  
High Binding Affinity

Abstract In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids (“chromatid selectivity”). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethylated Ds is unable to excise from a nonreplicating vector and that replication restores excision. A Ds element hemi-methylated on one DNA strand transposes in the absence of replication, whereas hemi-methylation of the complementary strand causes a >6.3-fold inhibition of Ds excision. Consistently in the active hemi-methylated state, the Ds ends have a high binding affinity for the transposase, whereas binding to inactive ends is strongly reduced. These results provide strong evidence for the above-mentioned model. Moreover, in the absence of DNA methylation, replication enhances Ds transposition in petunia protoplasts >8-fold and promotes formation of a predominant excision footprint. Accordingly, replication also has a methylation-independent regulatory effect on transposition.

Detection of Transgenic Tomato Leaf with LeETR1 Antisense Gene by Near-Infrared Spectroscopy

2010 ◽  
Vol 53 (1) ◽  
pp. 313-318 ◽  
Author(s):  
L. J. Xie ◽  
Y. B. Ying ◽  
M. L. Chen ◽  
T. J. Ying
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Tomato Leaf ◽  
Transgenic Tomato ◽  
Antisense Gene

Effects of pseudofype retrovirus containing human N-RAS antisense gene on the growth of human liver cancer LTNM4 transplanted in nude mice

1991 ◽  
Vol 3 (2) ◽  
pp. 22-26
Author(s):  
Xu Xiulan ◽  
Jia Libin ◽  
Zheng Yahai ◽  
Gan Chen ◽  
Gu Jianren ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Nude Mice ◽  
Human Liver ◽  
Antisense Gene ◽  
Human Liver Cancer

Alteration in the metastatic potential of ovarian cancer cells by transfection of the antisense gene of β-1,4-galactosyltransferase

2003 ◽  
Author(s):  
Hiroshi Yamashita ◽  
Kaneyuki Kubushiro ◽  
Jun Ma ◽  
Takuma Fujii ◽  
Katsumi Tsukazaki ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Metastatic Potential ◽  
Ovarian Cancer Cells ◽  
Antisense Gene

scholarly journals TGF-α antisense gene therapy inhibits head and neck squamous cell carcinoma growth in vivo

Gene Therapy ◽  
2000 ◽  
Vol 7 (22) ◽  
pp. 1906-1914 ◽  
Author(s):  
S Endo ◽  
Q Zeng ◽  
N A Burke ◽  
Y He ◽  
M F Melhem ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Antisense Gene

scholarly journals PERSPECTIVES OF ANTISENSE GENE THERAPY IN ORGAN TRANSPLANTATION

2014 ◽  
Vol 15 (1) ◽  
pp. 106
Author(s):  
O. N. Reznik ◽  
A. E. Skvortsov ◽  
D. O. Kuzmin ◽  
A. P. Tutin ◽  
A. O. Reznik
Keyword(s):  
Gene Therapy ◽  
Organ Transplantation ◽  
Antisense Gene

scholarly journals Analysis of transfer of tumor-inducing plasmids from Agrobacterium tumefaciens to Petunia protoplasts.

1985 ◽  
Vol 162 (3) ◽  
pp. 1030-1038 ◽  
Author(s):  
E L Virts ◽  
S B Gelvin
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Petunia Protoplasts

scholarly journals Identification and analysis of long non-coding RNAs that are involved in inflammatory process in response to transmissible gastroenteritis virus infection

2019 ◽  
Author(s):  
Xuelian Ma ◽  
Xiaomin Zhao ◽  
Kaili Wang ◽  
Xiaoyi Tang ◽  
Jianxiong Guo ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Messenger Rna ◽  
Binding Proteins ◽  
Differentially Expressed ◽  
Transmissible Gastroenteritis Virus ◽  
Intestinal Epithelial ◽  
Antisense Gene ◽  
Gastroenteritis Virus ◽  
Expression Of Genes ◽  
Transmissible Gastroenteritis

Abstract Abstract Background: Transmissible gastroenteritis virus (TGEV) infection can cause acute inflammation. Long noncoding RNAs (lncRNAs) play important roles in a number of biological process including inflammation response. However, whether lncRNAs participate in TGEV-induced inflammation in porcine intestinal epithelial cells (IPECs) is largely unknown. Results: In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in Mock and TGEV-infected porcine intestinal epithelial cell-jejunum 2 (IPEC-J2) cell line. A total of 106 lncRNAs were differentially expressed. Many differentially expressed lncRNAs act as elements to competitively attach microRNAs (miRNAs) which target to messenger RNA (mRNAs ) to mediate expression of genes that related to Toll-like receptors (TLRs), NOD-like receptors (NLRs), Tumor necrosis factor (TNF), and RIG-I-like receptor s (RLRs) pathways. Functional analysis of the binding proteins and the up/down-stream genes of the differentially expressed lncRNAs revealed that lncRNAs were principally related to inflammatory response. Meanwhile, we found that the differentially expressed lncRNA TCONS_00058367 might lead to a reduction of phosphorylation of transcription factor p65 (p-p65) in TGEV-infected IPEC-J2 cells by negatively regulating its antisense gene romyelocytic leukemia (PML ). Conclusions: The data showed that differentially expressed lncRNAs might be involved in inflammatory response induced by TGEV through acting as miRNA sponges, regulating their up/down-stream genes, or directly binding proteins.

scholarly journals Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence

2021 ◽  
Vol 118 (17) ◽  
pp. e2014783118
Author(s):  
Guangyong Ma ◽  
Jun-ichirou Yasunaga ◽  
Kazuya Shimura ◽  
Keiko Takemoto ◽  
Miho Watanabe ◽  
...  
Keyword(s):  
Viral Persistence ◽  
Protein Translation ◽  
Regulatory Function ◽  
Antisense Gene ◽  
Cell Leukemia Virus Type ◽  
Antisense Mrna ◽  
Human T Cell ◽  
Infected Cells ◽  
Hiv 1

Human retroviruses, including human T cell leukemia virus type 1 (HTLV-1) and HIV type 1 (HIV-1), encode an antisense gene in the negative strand of the provirus. Besides coding for proteins, the messenger RNAs (mRNAs) of retroviral antisense genes have also been found to regulate transcription directly. Thus, it has been proposed that retroviruses likely localize their antisense mRNAs to the nucleus in order to regulate nuclear events; however, this opposes the coding function of retroviral antisense mRNAs that requires a cytoplasmic localization for protein translation. Here, we provide direct evidence that retroviral antisense mRNAs are localized predominantly in the nuclei of infected cells. The retroviral 3′ LTR induces inefficient polyadenylation and nuclear retention of antisense mRNA. We further reveal that retroviral antisense RNAs retained in the nucleus associate with chromatin and have transcriptional regulatory function. While HTLV-1 antisense mRNA is recruited to the promoter of C-C chemokine receptor type 4 (CCR4) and enhances transcription from it to support the proliferation of HTLV-1–infected cells, HIV-1 antisense mRNA is recruited to the viral LTR and inhibits sense mRNA expression to maintain the latency of HIV-1 infection. In summary, retroviral antisense mRNAs are retained in nucleus, act like long noncoding RNAs instead of mRNAs, and contribute to viral persistence.

Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes

2016 ◽  
Vol 138 (29) ◽  
pp. 9097-9100 ◽  
Author(s):  
Xueguang Lu ◽  
Fei Jia ◽  
Xuyu Tan ◽  
Dali Wang ◽  
Xueyan Cao ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Polyethylene Glycol ◽  
Antisense Gene
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