In vitro study of human alveolar macrophage and peripheral blood mononuclear cell reactive oxygen-intermediates release induced by sulfur dioxide at different concentrations

Lung ◽  
1994 ◽  
Vol 172 (6) ◽  
Author(s):  
K. Kienast ◽  
J. M�ller-Quernheim ◽  
M. Knorst ◽  
S. Lubjuhn ◽  
R. Ferlinz
Keyword(s):  
Sulfur Dioxide ◽  
Peripheral Blood Mononuclear Cell ◽  
Alveolar Macrophage ◽  
Peripheral Blood ◽  
In Vitro Study ◽  
Reactive Oxygen Intermediates ◽  
Oxygen Intermediates ◽  
Peripheral Blood Mononuclear ◽  
Human Alveolar Macrophage

Head and Neck Squamous Cell Carcinoma Cell Line-Induced Suppression of in vitro Lymphocyte Proliferative Responses

1992 ◽  
Vol 106 (2) ◽  
pp. 149-158 ◽  
Author(s):  
Henry Lapointe ◽  
Howard Lampe ◽  
Diponkar Banerjee
Keyword(s):  
Squamous Cell Carcinoma ◽  
Peripheral Blood Mononuclear Cell ◽  
Tumor Cell ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear

Tumour-infiltrating lymphocytes (TILS) are often difficult to expand in vitro. In some cases this has been attributable to immunosuppression mediated by the elaboration of prostaglandins by either tumor cells or tumor-infiltrating monocytes. In this laboratory, freshly prepared TILs containing single-cell suspensions of head and neck tumors displayed both poor proliferation as well as minimal responsiveness to indomethacin-mediated reversal of immunosuppression. In order to investigate tumor-mediated immunosuppression further, a system was developed whereby a new cell line of head and neck squamous cell carcinoma was used to suppress allogeneic peripheral blood mononuclear cell proliferation in response to phytohemagglutinin (PHA) and interieukin-2 (IL-2). Tumor cells were able to suppress peripheral blood mononuclear cell (PBMNC) proliferation up to 95%. This suppressive effect was dependent on tumor cell number and was reversible by the use of higher concentrations of PHA, but not by increased concentrations of IL-2. Suppression was immediate when IL-2 was used as the stimulus for proliferation, but required extended lymphocyte/tumor cell contact when PHA was used. Flow cytometric analysis of tumor-exposed and PHA-stimulated PBMNCs revealed a decrease in both the number of cells expressing IL-2 receptors as well as the density of IL-2 receptors per cell. This pattern of suppression, as well as the reversibility of suppression by indomethacin, implicates prostaglandins in the mechanisms by which these tumor cells mediate immunosuppression.

scholarly journals Efek Pemberian Protein Rekombinan Fusi ESAT6-CFP10 Mycobacterium tuberculosis terhadap Persentase IL2 dan IL10 yang Dipresentasikan Sel T CD8 pada Kultur PBMC

2019 ◽  
Vol 30 (3) ◽  
pp. 202
Author(s):  
David Christianto ◽  
Tri Yudani Mardining Raras ◽  
Sumarno Sumarno ◽  
Maimun Zulhaidah Arthamin ◽  
Triwahju Astuti ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear

<p><br />Keberhasilan vaksin BCG dalam memberikan perlindungan terhadap tuberkulosis (TB) pada orang dewasa di Indonesia belum optimal (37%) sehingga diperlukan vaksin alternatif yang lebih efektif. Protein rekombinan fusi ESAT6-CFP10 merupakan kandidat vaksin yang potensial. Penelitian dilakukan untuk menguji efektifitas protein rekombinan fusi ESAT6-CFP10 dalam meningkatkan ekspresi IL2 dan IL10 sel T CD8 yang memainkan peran penting dalam respon imun melawan TB. Pengujian kandidat vaksin dilakukan secara in vitro pada peripheral blood mononuclear cell (PBMC) dari kelompok sehat endemik TB, kelompok kontak TB, dan kelompok pasien TB dengan melihat persentase IL2 dan IL10 CD8. Setiap kelompok diberi perlakuan tanpa antigen, PPD, dan protein rekombinan fusi ESAT6-CFP10. Persentase IL2 meningkat secara signifikan dari kelompok sehat, kontak TB, hingga Pasien TB. Sebaliknya peningkatan persentase IL2 antar kelompok yang dipaparkan PPD tidak signifikan secara statistik (p=0,396). Persentase IL10 tidak menunjukkan perbedaan yang signifikan antar kelompoknya baik tanpa paparan antigen (p=0,617), PPD (p=0,351), maupun protein rekombinan fusi ESAT6-CFP10 (p=0,257). Didapatkan persentase IL2 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,309), kelompok kontak TB (p=0,318), dan kelompok pasien TB (p=0,424). Demikian juga dengan persentase IL10 yang tidak berbeda secara signifikan antar perlakuan pada kelompok sehat (p=0,908), kelompok kontak TB (p=0,352), dan kelompok pasien TB (p=0,776). Hal ini menunjukkan bahwa protein fusi rekombinan ESAT6-CFP10 dapat meningkatkan persentase IL2 tetapi tidak dengan IL10 meskipun secara statistik tidak signifikan.</p>

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