Protein accumulation, RNA and soluble amino nitrogen content in developing endosperm of two varieties of Triticum aestivum with high and low protein seed

1977 ◽  
Vol 50 (2) ◽  
pp. 73-77 ◽  
Author(s):  
A. Brunori ◽  
P. Mannino ◽  
G. Ancora ◽  
A. Bozzini
Keyword(s):  
Triticum Aestivum ◽  
Nitrogen Content ◽  
Amino Nitrogen ◽  
Protein Accumulation ◽  
Low Protein

Antioxidant Activity of Silk Fibroin Peptide Hydrolyzed by Pancreatin

2014 ◽  
Vol 1081 ◽  
pp. 110-114 ◽  
Author(s):  
Zhen Zhu ◽  
Hua Yin ◽  
Yan An
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Nitrogen Content ◽  
Silk Fibroin ◽  
Radical Scavenging ◽  
Amino Nitrogen ◽  
Free Radical Scavenging ◽  
Hydrolysis Process ◽  
Superoxide Free Radical ◽  
Hydrolysis Of

This research adopts the pancreatin hydrolysis of silk fibroin active peptide, evaluate the antioxidant activity of hydrolysates. In the process of hydrolysis of silk fibroin, by measuring the amino nitrogen content of neutral formaldehyde titration method. Find the amino nitrogen content gradually stabilized at around 0.37g/L, and superoxide free radical scavenging rate changing with time fluctuation trend, superoxide free radical scavenging rate to a maximum of 65.03% at 220min.The use of silk fibroin hydrolysis process optimization,reaction time 160min, enzyme concentration4% , substrate concentration 20mg/ml, pH 8, temperature 38°C. The hydrolysis process under the hydrolysate on superoxide radical scavenging rate of 72.73%. The scavenging rate of hydroxyl radical is 47.24%. Red blood cell hemolysis induced by H2O2 inhibition rate was 24.30%.

The performance of calves given concentrate diets treated with formaldehyde

1973 ◽  
Vol 24 (4) ◽  
pp. 613 ◽  
Author(s):  
GJ Faichney ◽  
HL Davies
Keyword(s):  
Food Consumption ◽  
Protein Level ◽  
Rumen Fluid ◽  
Amino Nitrogen ◽  
Peanut Meal ◽  
Plasma Urea ◽  
Protein Levels ◽  
Low Protein ◽  
Formaldehyde Treatment ◽  
Protein Diets

Five groups of Friesian bull calves were given concentrate diets containing 70 % barley in which low (12 %), medium (15 %), and high (19%) protein levels were obtained by varying the amount of peanut meal included. The effects of protein level and of formaldehyde treatment of the complete diet at the low and medium protein levels were studied in terms of liveweight gain, voluntary food consumption, digestibility of the diet, ammonia nitrogen in rumen fluid, and urea and a-amino nitrogen in blood plasma. Observations were begun when the calves reached 70 kg liveweight and continued until they reached 130 kg liveweight. The calves given the low protein diets grew more slowly than those given the higher protein diets. The calves given the high protein diet grew no better than those given the medium protein diets. Formaldehyde treatment was associated with an increase in the rate of liveweight gain of 9% (P = 0.11) at the low protein level but had practically no effect at the medium protein level. The treatment did not adversely affect voluntary food consumption but was associated with decreases in the digestibility of nitrogen and in rumen ammonia levels and small increases in plasma urea levels.

DEVELOPMENT OF PARAMETERS FOR SALTING PACIFIC HERRING FILLET INTENDED FOR CHILDREN'S NUTRITION

Fisheries ◽  
2021 ◽  
Vol 2021 (1) ◽  
pp. 103-107
Author(s):  
Ekaterina Konopleva ◽  
Liubov Abramova ◽  
Valeria Gershunskaya ◽  
Maksim Arnautov
Keyword(s):  
Citric Acid ◽  
Nitrogen Content ◽  
Salt Content ◽  
Amino Nitrogen ◽  
Sodium Content ◽  
Pacific Herring ◽  
Children's Nutrition ◽  
Reduced Salt ◽  
Potassium Magnesium ◽  
Organoleptic Parameters

The aim of the work was to develop the parameters of the process of salting Pacific herring fillet with a reduced salt content, intended for children’s nutrition. For salting, food salt with a reduced sodium content, enriched with potassium, magnesium, was used. Based on the conducted studies, a rational formulation of the salting mixture was selected, consisting of 5 % salt with a reduced sodium content, 4% sugar, 0.35-0.50% citric acid. It was found that carrying out the salting process for 72-96 hours provides a low-salted fillet with a salt content of 3.5% with high organoleptic parameters, which are determined by the amino nitrogen content of 110-130 mg/100 g of the product and the buffering capacities of 40-45 degrees.

scholarly journals Physicochemical properties of three food proteins treated with transglutaminase

2004 ◽  
Vol 34 (4) ◽  
pp. 1219-1223 ◽  
Author(s):  
Luís Henrique de Barros Soares ◽  
Patrícia Melchionna Albuquerque ◽  
Francine Assmann ◽  
Marco Antônio Záchia Ayub
Keyword(s):  
Physicochemical Properties ◽  
Nitrogen Content ◽  
Soy Protein ◽  
Free Amino ◽  
Amino Nitrogen ◽  
Emulsifying Properties ◽  
Amino Groups ◽  
Emulsifying Activity ◽  
Food Proteins ◽  
Isolated Soy Protein

Three sources of food proteins were treated with microbial transglutaminase (EC 2.3.2.13) in order to assess changes in the physicochemical properties of reactivity, solubility, emulsification, and free amino groups of the formed polymers. Samples of lactic casein (LC), isolated soy protein (ISP), and hydrolysed animal protein (HAP), were incubated with the enzyme for one or two hours. LC and ISP showed a reduced solubility of 15% and 24% respectively, with HAP showing no alteration on solubility. Amino nitrogen content was 7%, 3% and 2% reduced for HAP, LC and ISP respectively. LC and ISP demonstrated lower emulsifying activity when they were enzymatically treated but the formed emulsions were stable, contrasting with HAP, which exhibited no changes in emulsifying properties.

The influence of diet on the nitrogenous components passing to the duodenum and through the lower ileum of sheep

1966 ◽  
Vol 166 (1002) ◽  
pp. 63-79 ◽  
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Nitrogen Content ◽  
Aspartic Acid ◽  
Total Nitrogen ◽  
Amino Nitrogen ◽  
Terminal Part ◽  
High Nitrogen ◽  
Low Nitrogen ◽  
Lower Ileum

Analyses of the alimentary contents flowing to the duodenum of sheep during 24 h show that when the sheep are consuming a low-nitrogen diet more total nitrogen and amino nitrogen pass to the duodenum than are eaten daily in the food whereas when the sheep are eating high nitrogen diets, less total nitrogen and less amino nitrogen pass to the duodenum. The disparity between the total nitrogen and amino nitrogen content of the diets largely disappeared by the time the alimentary contents reached the terminal part of the ileum. From 64 to 68% of the nitrogen entering the duodenum and 54 to 64% of the nitrogen in the ileal contents was in the form of amino nitrogen. Proportionately more of the amino nitrogen was in solution in the ileal contents than in the duodenal contents. Losses of amino acids in the stomach when a high-nitrogen diet was consumed were especially large for glutamic acid, aspartic acid, proline, arginine and leucine. They were least for cystine and threonine. Gains of amino acids in the stomach when low nitrogen diets were consumed were all substantial except for proline, where a loss was found when hay and flaked maize were given. When these changes are considered as proportions of the quantities eaten then trends are similar for all acids. Changes in the molar proportions of the amino acids present in hydrolysates of the duodenal and ileal contents are discussed together with the significance of these changes in relation to the nutrition of the sheep.

Effects of Yeast Extract on Poly-ε-Lysine Production in Batch Culture of Streptomyces Albulus

2011 ◽  
Vol 343-344 ◽  
pp. 1023-1028 ◽  
Author(s):  
Xiong Chen ◽  
Zhi Wang ◽  
Yong Ze Wang ◽  
Fa Tang Jiang ◽  
Dong Sheng Li ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Yeast Extract ◽  
Organic Nitrogen ◽  
Pantothenic Acid ◽  
Amino Nitrogen ◽  
Lysine Production ◽  
Dry Cell Weight ◽  
Brewers Yeast ◽  
Organic Nitrogen Source ◽  
Dry Cell

Yeast extract (YE) is a sole organic nitrogen source in a commonly medium 3G for Streptomyces albulus to produce poly-ε-lysine (ε-PL). In this study, five YEs from five commercial suppliers were used to evaluate their effects on the growth of Streptomyces albulus 213 and ε-PL production. YE from bakers’ yeast with the highest total nitrogen content (TN), α-amino nitrogen content (AN) or the ratio of AN/TN produced the highest yield of ε-PL, while YE from brewers’ yeast with the highest RNA content got the most dry cell weight (DCW). However, there was little correlation between TN, AN or AN/TN in YE and the yield of ε-PL. There was also weak correlation between RNA in YE and DCW. When 60% baker YE was mixed with 40% brewer YE, the yield of ε-PL reached 1.89 g/l and increased by 16% and 37% than those of baker YE and brewer YE, respectively. Furthermore, five kinds of vitamins and nine kinds of amino acids were screened to supplement baker YE. When 6 mg/l biotin, 5mg/l pantothenic acid, 0.7 g/l L-glutamine and 0.5 g/l L-arginine were mixed with baker YE in M3G, ε-PL reached maximum production respondent to 3.05 g/l that was 61.4% higher than control.

The role of the leaves in the accumulation of nitrogen by wheat during ear development

1963 ◽  
Vol 14 (6) ◽  
pp. 725 ◽  
Author(s):  
TF Neales ◽  
MJ Anderson ◽  
IF Wardlaw
Keyword(s):  
Nitrogen Content ◽  
External Factor ◽  
Amino Nitrogen ◽  
Wheat Plant ◽  
Dry Weight ◽  
Leaf Removal ◽  
Ear Development ◽  
Rooting Medium ◽  
Grain Nitrogen

When wheat plants were deprived of nitrogen in the rooting medium at anthesis there was a small, but significant, increase in the nitrogen content of the grain at maturity. It was shown that there was a greater migration of nitrogen from the leaves and stem to the ear in plants deprived of nitrogen than in those plants supplied with nitrogen throughout ear development. In an examination of the effects on grain nitrogen content of leaf removal at anthesis, it was shown that this treatment reduces the uptake of nitrogen into the culm and the nitrogen content of the grain at maturity. Ear shading treatments significantly reduced the total nitrogen content of the grain in one experiment. Ear shading also decreased the amino nitrogen, and increased the nitrate nitrogen, content of the ear. Variation of the grain dry weight per ear, induced by shading, by defoliation treatments, or by differences in variety, were positively correlated with grain nitrogen content. Either the movement of dry matter and nitrogen into the ear are interdependent, or they are both promoted by some external factor, such as rate of growth of the ear. The possible importance of the leaves of the wheat plant in promoting the uptake of nitrogen into the culm and in supplying nitrogen to the grain is discussed.

CHEMICAL CHANGES IN NITROGEN FRACTIONS OF PLANT JUICE ON EXPOSURE TO FROST

1931 ◽  
Vol 5 (3) ◽  
pp. 327-332 ◽  
Author(s):  
R. Newton ◽  
W. R. Brown ◽  
J. A. Anderson
Keyword(s):  
Winter Wheat ◽  
Nitrogen Content ◽  
Protein Content ◽  
Inorganic Nitrogen ◽  
Amino Nitrogen ◽  
Added Sugar ◽  
Nitrogen Fractions ◽  
The Press ◽  
Dilute Sodium Hydroxide ◽  
Increased Sensitivity

Exposure of the press-juice of unhardened winter wheat plants to frost caused a decrease in coagulable protein content, an increase in amino nitrogen content and an increased sensitivity to hydrolysis by dilute sodium hydroxide. Added sugar reduced the amount of change. No change resulting from freezing could be demonstrated in the inorganic nitrogen fractions. It is suggested that protein splitting as found in hardened plants may be the result of frost rather than an adaptation against it, and that the value of sugar as a protection to winter plants may lie partly in its ability to delay this action.

scholarly journals BIOCHEMICAL STUDIES ON THE FIBRINOLYTIC ACTIVITY OF HEMOLYTIC STREPTOCOCCI

1934 ◽  
Vol 60 (2) ◽  
pp. 255-267 ◽  
Author(s):  
R. L. Garner ◽  
William S. Tillett
Keyword(s):  
Nitrogen Content ◽  
Fibrinolytic Activity ◽  
Prolonged Exposure ◽  
Gradual Increase ◽  
Amino Nitrogen ◽  
The Other ◽  
Human Fibrinogen ◽  
Enzymatic Action ◽  
Other Hand ◽  
Biochemical Studies

The fibrinolysin of hemolytic streptococci exerts no hydrolytic action upon casein, gelatin, or peptone. The action on solid human fibrin is characterized by a small and gradual increase in the amino nitrogen content of the solution. The specific and special enzymatic action of fibrinolysin is contrasted with trypsin and with streptococcal peptase (4). Solutions of human fibrinogen, after brief incubation with fibrinolysin, lose the capacity to form fibrin. Solutions of rabbit fibrinogen, on the other hand, retain the property of transformation into fibrin, even after prolonged exposure to fibrinolysin. Qualitative tests, with solutions resulting from the action of streptococcal fibrinolysin on human fibrin, indicate that the end-product may be protein and that the degradation of the molecule is not great.

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