Potentiality of leaf sheath cells for regeneration of rice (Oryza sativa L.) plants

1980 ◽  
Vol 57 (4) ◽  
pp. 87-90 ◽  
Author(s):  
P. Bhattacharya ◽  
S. K. Sen

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.



2017 ◽  
Vol 05 (03) ◽  
Author(s):  
Dongryung Lee ◽  
Yoye Yu ◽  
Eunbyeol Koh ◽  
Hee-Jong Koh


1985 ◽  
Vol 63 (6) ◽  
pp. 1150-1156 ◽  
Author(s):  
S. Mukherji ◽  
A. K. Biswas

Penicillin promoted elongation of rice (Oryza sativa L.) seedlings; the relative promotion of shoot elongation was stronger than that of root elongation. Nucleic acids and protein were maintained at much higher levels in seedlings treated with penicillin, the increment of these metabolites being more pronounced in the embryo than in the endosperm. α-Amylase, RNase, and ATPase of endosperm and indoleacetic acid (IAA) oxidase activities of the entire seedlings were enhanced in penicillin treatments. Penicillin caused small increments in respiration rates of seedlings. IAA-induced growth inhibition of intact seedlings could be reversed completely to a growth promotion by joint application of penicillin with IAA. The length of the second leaf sheath of a dwarf rice cultivar was increased by penicillin which further enhanced the gibberellin-induced response. The levels of gibberellinlike substances were increased by penicillin treatments, whereas the IAA level remained unchanged. In penicillin-treated seedlings, more IAA was transported from endosperm to embryo. The stimulating effect of penicillin on rice seedling elongation was partially reversed by abscisic acid and the interaction appeared to be of a noncompetitive nature.



2011 ◽  
Vol 57 (No. 1) ◽  
pp. 26-33 ◽  
Author(s):  
I. Nemati ◽  
F. Moradi ◽  
S. Gholizadeh ◽  
M.A. Esmaeili ◽  
M.R. Bihamta

In order to investigate the solutes accumulation associated with salt tolerance of rice (Oryza sativa L.), two rice genotypes including IR651 (salt-tolerant) and IR29 (salt-sensitive) were grown hydroponically in the Youshida nutrient solution. Salinity treatment was imposed 3 weeks after sowing using NaCl in two levels 0 and 100 mmol. Samples were separately collected from the youngest (sixth) leaves, leaf sheaths and roots at 72 and 240 h after salinization; then Na<sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup>, Mg<sup>2+</sup>, P, Mn<sup>2+</sup>, Cl<sup>&ndash;</sup> and total soluble sugars concentration and Na+/K<sup>+</sup> ratio were determined. Total dry weight of both genotypes decreased with the application of NaCl. Salinity caused higher accumulation of Na<sup>+</sup> and Cl<sup>&ndash;</sup> in the sixth leaf and leaf sheath of IR29 than in IR651 while their concentration in root of IR651 was higher. K<sup>+</sup> concentration was decreased in the sixth leaf and leaf sheath of IR29 under NaCl stress. Reduction in Ca<sup>2+</sup> and Mg2+ concentrations were observed in sixth leaves of both genotypes. P concentration was increased in leaf sheath and root of IR29 under saline conditions while it showed no changes in IR651. Our results indicated that the tolerant genotype had mechanisms to prevent high Na<sup>+</sup> and Cl<sup>&ndash;</sup> accumulation in the sixth leaf. High total soluble sugars concentration in shoot of IR651 is probably for adjusting osmotic potential and better water uptake under salinity. These mechanisms help plant to avoid tissue death and enable to continue its growth and development under saline conditions. &nbsp;







1970 ◽  
Vol 5 (3) ◽  
pp. 391-403
Author(s):  
Wiwik Winarti ◽  
Eva Sartini Bayu ◽  
Revandy Iskandar Damanik

Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g.



ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.



2012 ◽  
Vol 2 (11) ◽  
pp. 13-14
Author(s):  
R. ARULMOZHI R. ARULMOZHI ◽  
◽  
Dr. A. MUTHUSWAMY Dr. A. MUTHUSWAMY


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