Urokinase receptor in breast cancer tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- and polyclonal antibodies

1995 ◽  
Vol 33 (3) ◽  
pp. 199-207 ◽  
Author(s):  
E. R�nne ◽  
G. H�yer-Hansen ◽  
N. Br�nner ◽  
H. Pedersen ◽  
F. Rank ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Breast Cancer Tissue ◽  
Urokinase Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts

Urokinase-type receptor in breast cancer tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- and polyclonal antibodies

1994 ◽  
Vol 8 ◽  
pp. 123 ◽  
Author(s):  
J. Grøndahl-Hansen ◽  
N. Brünner ◽  
G.M. Clark ◽  
K. Danø ◽  
G. Høyer-Hansen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Breast Cancer Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts ◽  
Type Receptor

Quantitative Assessment of HER-2/Neu Protein Concentration in Breast Cancer by Enzyme-Linked Immunosorbent Assay

2003 ◽  
Vol 18 (1) ◽  
pp. 13-20 ◽  
Author(s):  
V. Müller ◽  
C. Thomssen ◽  
C. Karakas ◽  
I. Eustermann ◽  
J. Ramirez Porras ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Amplification ◽  
Immunosorbent Assay ◽  
Immunohistochemical Staining ◽  
Protein Concentration ◽  
Tumor Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cancer Tissue ◽  
Tissue Extracts ◽  
Her 2

Purpose The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). Experimental design Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). Results Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p<0.0001, Mann-Whitney U test). A significant correlation between p185 concentration and immunohistochemical staining was observed (p<0.0001, Kruskal-Wallis test). In addition, p185 concentration measured by ELISA was correlated with the degree of HER-2/neu gene amplification determined by CISH. HER-2/neu-amplified tumors had significantly higher p185 concentrations (median value 181 ng/mg protein) than non-amplified tumors (median value 20 ng/mg; p<0.0001, Mann-Whitney U test). Conclusions ELISA-based measurement of HER-2/neu protein concentration in breast cancer tissue extracts is feasible and provides quantitative results for p185 protein concentrations that correlate closely with HER-2/neu immunoscore and gene amplification.

Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA

2006 ◽  
pp. 245-266 ◽  
Author(s):  
Manfred Schmitt ◽  
Alexandra S. Sturmheit ◽  
Anita Welk ◽  
Christel Schnelldorfer ◽  
Nadia Harbeck
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Protein Determination ◽  
Tissue Extracts ◽  
Pai 1

Classifying Breast Cancer Tissue by Raman Spectroscopy with One-dimensional Convolutional Neural Network

2021 ◽  
pp. 119732
Author(s):  
Danying Ma ◽  
Linwei Shang ◽  
Jinlan Tang ◽  
Yilin Bao ◽  
Juanjuan Fu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Neural Network ◽  
Raman Spectroscopy ◽  
Convolutional Neural Network ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
One Dimensional

scholarly journals Indication of high lipid content in epithelial-mesenchymal transitions of breast tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Siti Norbaini Sabtu ◽  
S. F. Abdul Sani ◽  
L. M. Looi ◽  
S. F. Chiew ◽  
Dharini Pathmanathan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Multivariate Analysis ◽  
Nucleic Acids ◽  
Raman Spectra ◽  
Cancer Progression ◽  
Component Analysis ◽  
Breast Cancer Tissue ◽  
Metabolic Changes ◽  
Cancer Tissue

AbstractThe epithelial-mesenchymal transition (EMT) is a crucial process in cancer progression and metastasis. Study of metabolic changes during the EMT process is important in seeking to understand the biochemical changes associated with cancer progression, not least in scoping for therapeutic strategies aimed at targeting EMT. Due to the potential for high sensitivity and specificity, Raman spectroscopy was used here to study the metabolic changes associated with EMT in human breast cancer tissue. For Raman spectroscopy measurements, tissue from 23 patients were collected, comprising non-lesional, EMT and non-EMT formalin-fixed and paraffin embedded breast cancer samples. Analysis was made in the fingerprint Raman spectra region (600–1800 cm−1) best associated with cancer progression biochemical changes in lipid, protein and nucleic acids. The ANOVA test followed by the Tukey’s multiple comparisons test were conducted to see if there existed differences between non-lesional, EMT and non-EMT breast tissue for Raman spectroscopy measurements. Results revealed that significant differences were evident in terms of intensity between the non-lesional and EMT samples, as well as the EMT and non-EMT samples. Multivariate analysis involving independent component analysis, Principal component analysis and non-negative least square were used to analyse the Raman spectra data. The results show significant differences between EMT and non-EMT cancers in lipid, protein, and nucleic acids. This study demonstrated the capability of Raman spectroscopy supported by multivariate analysis in analysing metabolic changes in EMT breast cancer tissue.

Quantitative estimation of BRCA1 protein expression in breast cancer tissue using the method of flow cytometry

2016 ◽  
Vol 61 ◽  
pp. S183
Author(s):  
E. Shestakova ◽  
E. Dudko ◽  
A. Grishanina ◽  
V. Kirsanov ◽  
N. Vichljantzeva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Protein Expression ◽  
Quantitative Estimation ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Brca1 Protein

Detection of DNA fragmentation in human breast cancer tissue by an antibody specific to single-stranded DNA

Breast Cancer ◽  
1998 ◽  
Vol 5 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Shunzo Kobayashi ◽  
Hirotaka Iwase ◽  
Yoshihiko Kawarada ◽  
Naoyuki Miura ◽  
Toshihiro Sugiyama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Fragmentation ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue ◽  
Single Stranded Dna
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