Quantitative estimation of BRCA1 protein expression in breast cancer tissue using the method of flow cytometry

2016 ◽  
Vol 61 ◽  
pp. S183
Author(s):  
E. Shestakova ◽  
E. Dudko ◽  
A. Grishanina ◽  
V. Kirsanov ◽  
N. Vichljantzeva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Protein Expression ◽  
Quantitative Estimation ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Brca1 Protein

scholarly journals QUANTITATIVE ESTIMATION OF BRCA1 PROTEIN EXPRESSION IN BREAST CANCER TISSUE USING THE METHOD OF FLOW CYTOMETRY

2016 ◽  
Vol 15 (4) ◽  
pp. 49-52
Author(s):  
T. A. Bogush ◽  
E. A. Dudko ◽  
E. A. Shestakova ◽  
A. N. Grishanina ◽  
E. A. Bogush ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Protein Expression ◽  
Quantitative Estimation ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Brca1 Protein

Breast cancer in Irish families with Lynch syndrome.

2012 ◽  
Vol 30 (4_suppl) ◽  
pp. 413-413
Author(s):  
E. J. Jordan ◽  
M. P. Farrell ◽  
R. M. Clarke ◽  
M. R. Kell ◽  
J. A. McCaffrey ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prostate Cancer ◽  
Lynch Syndrome ◽  
Protein Expression ◽  
Mismatch Repair ◽  
Hormone Receptor ◽  
Hormone Receptor Status ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Receptor Status

413 Background: Breast cancer is not a recognised malignant manifestation of Lynch Syndrome which includes colorectal, endometrial, gastric, ovarian and upper urinary tract tumours. In this study we report the prevalence of breast cancer in Irish Lynch Syndrome families and determine immunohistochemical expression of mismatch repair proteins (MMR) in available breast cancer tissue. Methods: Breast cancer prevalence was determined among Lynch Syndrome kindreds from two institutions in Ireland, and a genotype phenotype correlation was investigated. One kindred was omitted due to the presence of a biallelic MMR and BRCA1 mutation. The clinicopathological data that was collected on breast cancer cases included age of onset, morphology, and hormone receptor status. Immunohistochemical staining was performed for MLH1, MSH2, MSH6, and PMS2 on all available breast cancer tissue from affected individuals. Results: The distribution of MMR mutations seen in 16 pedigrees was as follows; MLH1 (n=5), MSH2 (7), MSH6 (3), PMS2 (1). Sixty cases of colorectal cancer and 14 cases of endometrial cancer were seen. Seven breast cancers (5 invasive ductal and 2 invasive lobular cancers) and 1 case of ductal carcinoma in situ were reported in 7 pedigrees. This compared with 4 cases of prostate cancer. Six MSH2 mutations and 1 MSH6 mutation were identified in the 7 Lynch syndrome kindreds. Median age of breast cancer diagnosis was 49 years (range 38-57). Hormone receptor status is available on 3 breast cancer cases at time of abstract submission; all were ER positive and HER 2 negative. All cases had grade 2 or 3 tumours. Final results of immunohistochemistry for mismatch repair protein expression on breast cancer samples are pending and will be reported at the meeting. One breast cancer has been tested to date and demonstrated loss of MSH2 protein expression in an individual carrying an MSH2 mutation. Conclusions: Breast cancer occurred at an early age and was more common than prostate cancer in Irish Lynch Syndrome pedigrees. All reported breast cancer cases were in kindreds with MSH2 or MSH6 mutations. Enhanced breast cancer screening may be warranted in certain Lynch Syndrome kindreds.

scholarly journals Localization of BRCA1 protein in breast cancer tissue and cell lines with mutations

2013 ◽  
Vol 13 (1) ◽  
pp. 70 ◽  
Author(s):  
Natalie Tulchin ◽  
Leonard Ornstein ◽  
Steven Dikman ◽  
James Strauchen ◽  
Shabnam Jaffer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Brca1 Protein

scholarly journals Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2731
Author(s):  
Andjelika Kalezic ◽  
Mirjana Udicki ◽  
Biljana Srdic Galic ◽  
Marija Aleksic ◽  
Aleksandra Korac ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Adipose Tissue ◽  
Protein Expression ◽  
Pentose Phosphate Pathway ◽  
Warburg Effect ◽  
Normal Weight ◽  
Pentose Phosphate ◽  
Breast Cancer Tissue ◽  
Metabolic Reprogramming ◽  
Cancer Tissue

Typical features of the breast malignant phenotype rely on metabolic reprogramming of cancer cells and their interaction with surrounding adipocytes. Obesity is strongly associated with breast cancer mortality, yet the effects of obesity on metabolic reprogramming of cancer and cancer-associated adipose tissue remain largely unknown. Paired biopsies of breast tumor tissue and adipose tissue from premenopausal women were divided according to pathohistological analyses and body mass index on normal-weight and overweight/obese with benign or malignant tumors. We investigated the protein expression of key regulatory enzymes of glycolysis, pentose phosphate pathway (PPP), and glycogen synthesis. Breast cancer tissue showed a simultaneous increase in 5′-AMP-activated protein kinase (AMPK) protein expression with typical features of the Warburg effect, including hexokinase 2 (HK 2) overexpression and its association with mitochondrial voltage-dependent anion-selective channel protein 1, associated with an overexpression of rate-limiting enzymes of glycolysis (phosphofructokinase 1—PFK-1) and pentose phosphate pathway (glucose-6-phosphate dehydrogenase—G6PDH). In parallel, cancer-associated adipose tissue showed increased AMPK protein expression with overexpression of HK 2 and G6PDH in line with increased PPP activity. Moreover, important obesity-associated differences in glucose metabolism were observed in breast cancer tissue showing prominent glycogen deposition and higher glycogen synthase kinase-3 protein expression in normal-weight women and higher PFK-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression in overweight/obese women. In conclusion, metabolic reprogramming of glycolysis contributes to tissue-specific Warburg effect in breast cancer and cancer-associated adipose tissue.

Increased FNDC5/IRISIN protein expression in breast cancer tissue is associated with obesity in postmenopausal women

2021 ◽  
pp. jclinpath-2020-207249
Author(s):  
María Elena Tejeda ◽  
Patricia Canto ◽  
Alberto Tenorio-Torres ◽  
Letica Orozco-Arguelles ◽  
Ramón Mauricio Coral-Vázquez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Postmenopausal Women ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Tissue ◽  
Breast Cancer Cell Lines ◽  
Cancer Tissue

AimTo analyse the fibronectin type III domain containing 5 (FNDC5)/irisin expression in tumour tissue of postmenopausal women presenting breast cancer and different body mass indexes (BMIs), proposing that obesity deregulates the expression of FNDC5/irisin at the breast tumour level. In addition, we investigated if different breast cancer cell lines are capable to synthesise this protein.MethodsA total of 150 postmenopausal women (50 with a normal BMI, 50 presenting overweight and 50 having obesity) diagnosed with operable breast cancer were included. FNDC5/irisin expression was determined by immunohistochemistry or by immunocytochemistry. Qualitative analysis of protein expression was performed by the H-Score method, through ImageJ’s IHC Profiler software. Statistical analyses were carried out using STATA V.14.0 (Texas, USA); p value<0.05 was accepted as statistically significant. Statistical power of the study was >80% with a p<0.05.ResultsFNDC5/irisin expression in breast cancer tissue of postmenopausal women with obesity was significantly increased when compared with FNDC5/irisin expression in women with a normal BMI (p=0.001). Furthermore, three breast cancer cell lines studied were capable to synthesise and express FNDC5/irisin, being the BT-474 cell line the one that exhibited the highest intensity of expression.ConclusionsOur results confirm that women with breast cancer and obesity exhibit an increased irisin expression in their tumorous tissue compared with women with breast cancer and normal BMI. Likewise, in vitro breast cancer cell lines have the capacity to synthesise and express FNDC5/irisin, without any extracellular stimuli, however the microenvironment surrounding these cells in vivo participates in its regulation.

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