The effect of ovariectomy of serum amino acids and cholesterol in the rat

Amino Acids ◽  
1994 ◽  
Vol 6 (1) ◽  
pp. 37-45 ◽  
Author(s):  
G. Frauscher ◽  
G. Lubec
Keyword(s):  
Amino Acids ◽  
Serum Amino Acids

Effects of glucose, pyruvate, lactate, and amino acids on muscle protein synthesis

1982 ◽  
Vol 242 (3) ◽  
pp. E184-E192 ◽  
Author(s):  
M. P. Hedden ◽  
M. G. Buse
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Creatine Phosphate ◽  
Serum Amino Acids ◽  
14Co2 Production ◽  
Beta Hydroxybutyrate ◽  
Free Media ◽  
Effect Of Glucose

Protein synthesis was measured in rat diaphragms incubated with serum amino acids + 0.35 mM L-[2,6-3H]tyrosine and different energy-yielding substrates. Muscles incubated with 5.5 mM glucose (with or without actinomycin D) synthesized more protein than those incubated with 11 mM pyruvate or 11 mM lactate. Tissue ATP decreased during incubation with lactate, but pyruvate maintained ATP, ADP, and creatine phosphate as well as glucose. Glucose 6-phosphate decreased in muscles incubated in glucose-free media. 14CO2 production from substrates was [1-14C]pyruvate greater than [1-14C]lactate greater than [3,4-14C]glucose. Intracellular lactate/pyruvate was measured to assess cytoplasmic free NADH/NAD+; the effect of different media on these ratios was lactate greater than glucose = lactate + pyruvate greater than pyruvate + glucose greater than pyruvate. Lactate + pyruvate (8.8 + 2.2 mM) supported protein synthesis better than pyruvate and as well as glucose. Adding glucose to pyruvate accelerated protein synthesis and increased NADH/NAD+. Iodoacetate (0.1 mM) inhibited glycolytic NAD reduction and abolished the stimulatory effect of glucose on protein synthesis in the presence of pyruvate. Supplementation of pyruvate media with 1 mM leucine or isoleucine stimulated protein synthesis, but beta-hydroxybutyrate, malate, alpha-ketoisocaproate, and all other amino acids were ineffective. The cytoplasmic redox potential may act as a translational modulator of protein synthesis in skeletal muscle.

Effect of protein restriction on15N transfer from dietary [15N]alanine and [15N]Spirulina platensisinto urea

2001 ◽  
Vol 281 (2) ◽  
pp. E349-E356 ◽  
Author(s):  
Mazen J. Hamadeh ◽  
L. John Hoffer
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Test Meal ◽  
High Protein Diet ◽  
Normal Subjects ◽  
Insulin Dependent Diabetes Mellitus ◽  
Protein Restriction ◽  
Dependent Diabetes Mellitus ◽  
Serum Amino Acids ◽  
Normal Men

Six normal men consumed a mixed test meal while adapted to high (1.5 g · kg−1· day−1) and low (0.3 g · kg−1· day−1) protein intakes. They completed this protocol twice: when the test meals included 3 mg/kg of [15N]alanine ([15N]Ala) and when they included 30 mg/kg of intrinsically labeled [15N] Spirulina platensis([15N]SPI). Six subjects with insulin-dependent diabetes mellitus (IDDM) receiving conventional insulin therapy consumed the test meal with added [15N]Ala while adapted to their customary high-protein diet. Protein restriction increased serum alanine, glycine, glutamine, and methionine concentrations and reduced those of leucine. Whether the previous diet was high or low in protein, there was a similar increase in serum alanine, methionine, and branched-chain amino acid concentrations after the test meal and a similar pattern of15N enrichment in serum amino acids for a given tracer. When [15N]Ala was included in the test meal,15N appeared rapidly in serum alanine and glutamine, to a minor degree in leucine and isoleucine, and not at all in other circulating amino acids. With [15N]SPI, there was a slow appearance of the label in all serum amino acids analyzed. Despite the different serum amino acid labeling, protein restriction reduced the postmeal transfer of dietary15N in [15N]Ala or [15N]SPI into [15N]urea by similar amounts (38 and 43%, respectively, not significant). The response of the subjects with IDDM was similar to that of the normal subjects. Information about adaptive reductions in dietary amino acid catabolism obtained by adding [15N]Ala to a test meal appears to be equivalent to that obtained using an intrinsically labeled protein tracer.

scholarly journals IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Yong Ma ◽  
Hongmei Jiang ◽  
Jun Fang ◽  
Gang Liu
Keyword(s):  
Amino Acids ◽  
Intestinal Inflammation ◽  
Great Influence ◽  
Basal Diet ◽  
Immune Defense ◽  
Inflammatory Factors ◽  
Adaptation Period ◽  
Serum Samples ◽  
Serum Amino Acids ◽  
Inflammatory Bowel

Background and Objective. Bioactive peptides exert great influence in animals and human health by targeting gastrointestinal tracts. The colitis model of mice was induced by dextran sulfate sodium (DSS). Thirty-two 8-week-old mice weighing 23 g on average were randomly assigned to four groups of 8 each: mice fed basal diet (CON), mice fed basal diet with 5% DSS (DSS), mice fed 0.03% IRW with 5% DSS (IRW-DSS), and mice fed 0.03% IRW with 5% DSS (IQW-DSS). After an adaptation period of 3 days, on day 8, all mice were slaughtered. Serum samples were collected to determine the level of amino acids; colonic tissue was quick-frozen for the determination of gene expression. Methods. The aim of this study was to assess the ability of two kinds of peptides (IRW and IQW) to repair intestinal inflammatory in the DSS-induced model in accordance with serum amino acids and intestinal inflammatory factors. Results. The results demonstrated that the addition of IRW and IQW had a mitigating effect on DSS-induced intestinal inflammation. The level of Asp decreased in the serum of mice supplemented with IRW-DSS (P<0.05), and IQW enhanced the level of Leu, but lowered the level of Ser (P<0.05). IQW and IRW addition reduced the level of TNF-α and IL-17 (P<0.05). No other significant effects were observed. Conclusions. The present study demonstrated that intracolic administration of IRW and IQW might be a novel option for preventing inflammatory bowel disease via regulating the level of serum amino acid and enhancing the intestinal immune defense.

Blood serum amino acids of nonpregnant women who have given birth to children with gross developmental malformations

1970 ◽  
Vol 108 (8) ◽  
pp. 1213-1216
Author(s):  
Ireneusz Roszkowski ◽  
Janina Iwanska ◽  
Leopold Myszkowski ◽  
Jerzy Brzeski ◽  
Alicja Schmalhofer
Keyword(s):  
Amino Acids ◽  
Blood Serum ◽  
Serum Amino Acids ◽  
Developmental Malformations

Serum Amino Acids, Liver Status, and Antiepileptic Drug Therapy in Epilepsy

Epilepsia ◽  
1993 ◽  
Vol 34 (2) ◽  
pp. 347-354 ◽  
Author(s):  
Marie Luise Rao ◽  
Hermann Stefan ◽  
Christof Scheid ◽  
Anja D. S. Kuttler ◽  
Walter Froscher
Keyword(s):  
Amino Acids ◽  
Drug Therapy ◽  
Antiepileptic Drug ◽  
Serum Amino Acids ◽  
Antiepileptic Drug Therapy

scholarly journals Histidine and arginine are associated with inflammation and oxidative stress in obese women

2011 ◽  
Vol 108 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Yu-Cun Niu ◽  
Ren-Nan Feng ◽  
Yan Hou ◽  
Kang Li ◽  
Zhen Kang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Human Case ◽  
Obese Women ◽  
Negatively Associated ◽  
Reactive Protein ◽  
Serum Amino Acids ◽  
The Difference ◽  
Oxidative Biomarkers ◽  
And Oxidative Stress

The aims of the present study were to examine the serum amino acid profiles in obese and non-obese women and investigate the relationships between the serum amino acids and inflammation and oxidative stress in a human case–control study. Serum amino acids, inflammatory biomarkers (C-reactive protein and IL-6) and oxidative biomarkers (superoxide dismutase, malondialdehyde and glutathione peroxidase) were measured and compared in 235 obese women and 217 non-obese controls. The relationships between serum amino acids and inflammatory and oxidative biomarkers were examined using multiple linear regression. Among the amino acids determined, serum histidine, arginine, threonine, glycine, lysine and serine were found to be significantly lower in obese women as compared to non-obese controls (P < 0·001). The difference was the greatest for histidine (P < 0·001). In obese women, both histidine and arginine were negatively associated with inflammation and oxidative stress. In non-obese controls, histidine was negatively associated with oxidative stress. The findings in this study indicate that the metabolism of amino acids is abnormal in obese women in whom histidine and arginine have close relationships with inflammation and oxidative stress.

Free serum amino acids in patients with advanced cervical carcinoma

1976 ◽  
Vol 4 (3) ◽  
pp. 311-313 ◽  
Author(s):  
Emery A. Wilson ◽  
A.D. Sprague ◽  
Melvin E. Hurst ◽  
J.W. Roddick
Keyword(s):  
Amino Acids ◽  
Cervical Carcinoma ◽  
Advanced Cervical Carcinoma ◽  
Serum Amino Acids ◽  
Free Serum

Serum amino acids in weight-losing patients with cancer and tuberculosis

1983 ◽  
Vol 19 (6) ◽  
pp. 711-715 ◽  
Author(s):  
L. Levin ◽  
W. Gevers ◽  
L. Jardine ◽  
F.J.M. De Guel ◽  
E.J. Duncan
Keyword(s):  
Amino Acids ◽  
Patients With Cancer ◽  
Serum Amino Acids

Disappearance of glucagonoma rash after surgical resection, but not during dietary normalization of serum amino acids

1984 ◽  
Vol 39 (3) ◽  
pp. 351-355 ◽  
Author(s):  
C Abraira ◽  
M DeBartolo ◽  
R Katzen ◽  
A M Lawrence
Keyword(s):  
Amino Acids ◽  
Surgical Resection ◽  
Serum Amino Acids
Sign in / Sign up

Export Citation Format

Share Document