Riboprinting ofNaegleria spp.: Small-subunit versus large-subunit rDNA

1994 ◽  
Vol 80 (3) ◽  
pp. 230-234 ◽  
Author(s):  
J. F. De Jonckheere
Keyword(s):  
Large Subunit ◽  
Small Subunit ◽  
Large Subunit Rdna

On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus, and relationships within Ophiostoma based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Data Set ◽  
Rdna Sequences ◽  
Morphological Criteria ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Large Subunit Rdna

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.

Molecular and morphological characterisation of Paurodontella persica n. sp. (Hexatylina: Sphaerulariidae) from soil in Iran

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Body Contour ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Rdna Gene ◽  
Posterior Extension ◽  
Large Subunit Rdna ◽  
A New Species

A new species ofPaurodontella,P. persican. sp., collected from the Alborz rangelands of Iran, is described and illustrated. The new species is characterised by its body length of 506 (416-599) μm and 424 (388-461) μm in female and male, respectively, four incisures in lateral field, lip region annulated and continuous with body contour, total stylet 6.2 (5.5-7.0) μm long. Basal pharyngeal bulb with small posterior extension projecting reaching to the intestine. Excretory pore situated at the level of basal pharyngeal bulb region. Post-uterine sac short, 4.5 (3.0-6.0) μm long and uterus with an offset diverticulum. Tail of both sexes similar, conoid pointed, terminus tapering into a long pointed mucron. Male with adanal bursa and spicules 16.5 (16.0-17.0) μm long. The new species comes close in morphology and morphometrics to four known species of the genus, namelyP. auriculata,P. myceliophaga,P. minutaandP. niger. Molecular analyses of the partial small subunit rDNA gene (SSU), D2-D3 expansion segments of the large subunit rDNA gene (LSU) and internal transcribed spacer (ITS) revealed this to be a new species.

scholarly journals Blastobotrys americana sp. nov., Blastobotrys illinoisensis sp. nov., Blastobotrys malaysiensis sp. nov., Blastobotrys muscicola sp. nov., Blastobotrys peoriensis sp. nov. and Blastobotrys raffinosifermentans sp. nov., novel anamorphic yeast species

2007 ◽  
Vol 57 (5) ◽  
pp. 1154-1162 ◽  
Author(s):  
Cletus P. Kurtzman
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Its2 Rdna ◽  
Small Subunit Rdna ◽  
Anamorphic Yeast ◽  
Large Subunit Rdna ◽  
Cytochrome Oxidase Ii

The genus Blastobotrys, which now includes species previously assigned to the synonymous genera Arxula and Sympodiomyces, represents the anamorph of the ascosporogenous genus Trichomonascus. Six novel species are proposed for assignment to Blastobotrys. They were detected from their unique nucleotide sequences in large-subunit rDNA, ITS1–5.8S–ITS2 rDNA, mitochondrial small-subunit rDNA and the cytochrome oxidase II gene. The proposed novel species are Blastobotrys americana sp. nov. (type strain NRRL Y-6844T=CBS 10337T; substrate unknown; Kansas, USA), Blastobotrys illinoisensis sp. nov. (type strain NRRL YB-1343T=CBS 10339T; from forest debris; Illinois, USA), Blastobotrys malaysiensis sp. nov. (type strain NRRL Y-6417T=CBS 10336T; from soil; Malaysia), Blastobotrys muscicola sp. nov. (type strain NRRL Y-7993T=CBS 10338T; from moss; Louisiana, USA), Blastobotrys peoriensis sp. nov. (type strain NRRL YB-2290T=CBS 10340T; from a fungus; Peoria, IL, USA) and Blastobotrys raffinosifermentans sp. nov. (type strain NRRL Y-27150T=CBS 6800T; substrate unknown).

Revisiting the phylogeny of Ocellularieae, the second largest tribe within Graphidaceae (lichenized Ascomycota: Ostropales)

Phytotaxa ◽  
2014 ◽  
Vol 189 (1) ◽  
pp. 52 ◽  
Author(s):  
Ekaphan Kraichak ◽  
Sittiporn Parnmen ◽  
Robert Lücking ◽  
EIMY RIVAS PLATA ◽  
André Aptroot ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Small Subunit ◽  
New Combinations ◽  
New Name ◽  
Small Subunit Rdna ◽  
Large Subunit Rdna ◽  
First Time

We present an updated 3-locus molecular phylogeny of tribe Ocellularieae, the second largest tribe within subfamily Graphidoideae in the Graphidaceae. Adding 165 newly generated sequences from the mitochondrial small subunit rDNA (mtSSU), the nuclear large subunit rDNA (nuLSU), and the second largest subunit of the DNA-directed RNA polymerase II (RPB2), we currently distinguish 218 species among the sequenced material, including the outgroup. This corresponds to almost half the species at this point recognized within this tribe. The newly generated sequences include 23 newly described species and one newly described genus published elsewhere in this volume. For the first time, Sarcographina cyclospora Müll. Arg., in spite of its distinctly lirellate ascomata, is shown to belong in tribe Ocellularieae, as strongly supported sister to Ocellularia inturgescens (Müll. Arg.) Mangold. The following six new combinations are proposed: Melanotrema lynceodes (Nyl.) Rivas Plata, Lücking & Lumbsch, Ocellularia curranii (Vain.) Kraichak, Lücking & Lumbsch, O. khasiana (Patw. & Nagarkar) Kraichak, Lücking & Lumbsch, O. cinerea (Müll. Arg.) Kraichak, Lücking & Lumbsch, O. erodens (R. C. Harris) Kraichak, Lücking & Lumbsch, and O. laeviuscula (Nyl) Kraichak, Lücking & Lumbsch. Further, the new name Ocellularia hernandeziana Kraichak, Lücking & Lumbsch is introduced for Myriotrema ecorticatum. The nomenclatural status of the name Ocellularia microstoma is clarified.

scholarly journals Species delimitation for the molecular taxonomy and ecology of the widely distributed microbial eukaryote genus Euplotes (Alveolata, Ciliophora)

2018 ◽  
Vol 285 (1871) ◽  
pp. 20172159 ◽  
Author(s):  
Yan Zhao ◽  
Zhenzhen Yi ◽  
Alan Warren ◽  
Weibo B. Song
Keyword(s):  
Species Delimitation ◽  
High Throughput Sequencing ◽  
Large Subunit ◽  
Molecular Taxonomy ◽  
Integrative Taxonomy ◽  
Small Subunit ◽  
Sensu Stricto ◽  
Gene Markers ◽  
Morphological Differences ◽  
Large Subunit Rdna

Recent advances in high-throughput sequencing and metabarcoding technologies are revolutionizing our understanding of the diversity and ecology of microbial eukaryotes (protists). The interpretation of protist diversity and the elucidation of their ecosystem function are, however, impeded by problems with species delimitation, especially as it applies to molecular taxonomy. Here, using the ciliate Euplotes as an example, we describe approaches for species delimitation based on integrative taxonomy by using evolutionary and ecological perspectives and selecting the most appropriate metabarcoding gene markers as proxies for species units. Our analyses show that: Euplotes ( sensu lato ) comprises six distinct clades, mainly as result of ecological speciation; the validity of the genera Euplotes ( sensu stricto ), Euplotoides , Euplotopsis and Moneuplotes are not supported; the vannus -type group, which includes species without distinct morphological differences, seems to be undergoing incipient speciation and contains cryptic species; the hypervariable V4 region of the small subunit rDNA and D1–D2 region of the large subunit rDNA are the promising candidates for general species delimitation in Euplotes .

High nucleotide divergence in a dimorphic parasite with disparate hosts

Zootaxa ◽  
2007 ◽  
Vol 1636 (1) ◽  
pp. 59-68 ◽  
Author(s):  
JEYARANEY KATHIRITHAMBY ◽  
JOSEPH J. GILLESPIE ◽  
EVA JIMENEZ-GURI ◽  
ANTHONY I. COGNATO ◽  
J. SPENCER JOHNSTON
Keyword(s):  
Evolutionary Biology ◽  
Large Subunit ◽  
Small Subunit ◽  
Sexually Dimorphic ◽  
Red Imported Fire Ant ◽  
Los Tuxtlas ◽  
Variable Regions ◽  
Morphological Stasis ◽  
Solenopsis Invicta Buren ◽  
Large Subunit Rdna

Morphologically cryptic male C. fenyesi sensu lato are found parasitic in different ant hosts over a wide geographical range. We use ribosomal DNA (rDNA) primary sequence and predicted rRNA secondary structure to compare between the second expansion segment (D2) of the nuclear large subunit rDNA (28S) and the entire nuclear small subunit rDNA (18S) of the male and the sexually dimorphic, neotenic female of Caenocholax fenyesi waloffi Kathirithamby & Johnston from Los Tuxtlas, Mexico, with that of the morphologically identical male C. f. texensis Kathirithamby & Johnston (Myrmecolacidae) from Texas, USA. In Los Tuxtlas the male C. f. waloffi parasitizes the ant, Dolichoderus bispinosus Olivier, while the female parasitizes the cricket, Macroanaxipha macilenta (Saussure). In Texas, the male C. f. texensis parasitizes the red imported fire ant, Solenopsis invicta Buren. The compared sequences show similar unusual secondary structures in the rRNA variable regions, but with approximately 14% overall divergence between Mexican and Texan specimens (15.5% divergence after correction for multiple substitutions). Our findings open a new opportunity in evolutionary biology to investigate speciation under a mechanism of morphological stasis and high genetic divergence in a unique parasite that is not only profoundly sexually dimorphic but wherein the sexes specialize in entirely different niches (hosts).

scholarly journals Molecular phylogeny reveals the true colours of Myeloconidaceae (Ascomycota: Ostropales)

2014 ◽  
Vol 27 (1) ◽  
pp. 38 ◽  
Author(s):  
Matthew P. Nelsen ◽  
Robert Lücking ◽  
Carrie J. Andrew ◽  
André Aptroot ◽  
Marcela E. S. Cáceres ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Species Concept ◽  
Large Subunit ◽  
Molecular Data ◽  
Small Subunit ◽  
Separate Genus ◽  
The Family ◽  
Single Genus ◽  
Ascospore Septation ◽  
Large Subunit Rdna

The lichen-forming fungal family Myeloconidaceae, with the single genus Myeloconis, has been suggested to share affinities with Porinaceae (Lecanoromycetes: Ostropales). We examined its position relative to this family by using molecular data from the mitochondrial small-subunit and nuclear large-subunit rDNA. Our results revealed that Myeloconis forms a monophyletic group nested within Porinaceae, closely related to Porina farinosa. Neither Porina s.str. nor Clathroporina sensu Harris form monophyletic groups; instead, two strongly supported clades were recovered, which differ in ascospore septation (septate v. muriform), with the clade producing muriform ascospores including Myeloconis. We therefore reduce Myeloconidaceae to synonymy with Porinaceae; however, because generic delimitations within Porinaceae remain unclear, we retain Myeloconis as a separate genus within the family. The species concept currently used in the genus, based largely on secondary metabolites and ascospore measurements, is supported by the phylogeny.

scholarly journals Candida bromeliacearum sp. nov. and Candida ubatubensis sp. nov., two yeast species isolated from the water tanks of Canistropsis seidelii (Bromeliaceae)

2005 ◽  
Vol 55 (5) ◽  
pp. 2213-2217 ◽  
Author(s):  
Carla C. C. Ruivo ◽  
Marc-André Lachance ◽  
Carlos A. Rosa ◽  
Maurício Bacci ◽  
Fernando C. Pagnocca
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Small Subunit ◽  
Eastern Brazil ◽  
Small Subunit Rdna ◽  
Large Subunit Rdna ◽  
Ascomycetous Yeasts ◽  
Water Tanks ◽  
Type Strains

Strains belonging to two novel yeast species, Candida bromeliacearum and Candida ubatubensis, were isolated from the bromeliad tank of Canistropsis seidelii (Bromeliaceae) in a sandy coastal plain (restinga) ecosystem site in an Atlantic rainforest of south-eastern Brazil. These species were genetically distinct from all other currently accepted ascomycetous yeasts, based on sequence divergence in the D1/D2 domains of the large-subunit rDNA and in the small-subunit rDNA. The species occupy basal positions in the Metschnikowiaceae clade. The type strains are Candida bromeliacearum UNESP 00-103T (=CBS 10002T=NRRL Y-27811T) and Candida ubatubensis UNESP 01-247RT (=CBS 10003T=NRRL Y-27812T).

Isolation and in vitro cultivation of the aphid pathogenic fungusEntomophthora planchoniana

2001 ◽  
Vol 47 (12) ◽  
pp. 1082-1087 ◽  
Author(s):  
Florian M Freimoser ◽  
Annette B Jensen ◽  
Urs Tuor ◽  
Markus Aebi ◽  
Jørgen Eilenberg
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Large Subunit ◽  
Small Subunit ◽  
In Vitro Cultivation ◽  
Small Subunit Rdna ◽  
Fetal Bovine ◽  
Large Subunit Rdna ◽  
First Time

Entomophthora planchoniana is an important fungal pathogen of aphids. Although Entomophthora chromaphidis has been considered a synonym for E. planchoniana, the two species are now separated, and E. planchoniana is reported not to grow in vitro. In this paper, we describe for the first time the isolation and cultivation of this species. Entomophthora planchoniana was isolated from a population of Ovatus crataegarius (Homoptera, Aphididae), which was infected by E. planchoniana only. The isolates did not sporulate, but the sequence of the small subunit rDNA and the restriction fragment length polymorphism patterns of the first part of the large subunit rDNA and the ITS II region confirm that the isolates were E. planchoniana. The isolated fungus grew in a medium consisting of Grace's insect cell culture medium supplemented with lactalbumin hydrolysate, yeastolate, and 10% fetal bovine serum or in GLEN medium with 10% fetal bovine serum. Vegetative cells of E. planchoniana were long and club-shaped and did not stain with Calcofluor, thus suggesting that they were protoplasts.Key words: biological control, entomopathogen, Entomophthorales, protoplast, Zygomycetes.

A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Shanxi province, China

Zootaxa ◽  
2013 ◽  
Vol 3630 (3) ◽  
pp. 561-570 ◽  
Author(s):  
YU MEI XU ◽  
ZENG QI ZHAO ◽  
JIAN MING WANG ◽  
JING WU ZHENG
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Ventral Side ◽  
Small Subunit ◽  
Shanxi Province ◽  
Cervical Region ◽  
Large Subunit Rdna ◽  
Using Data ◽  
A New Species ◽  
Shape And Size

Tripylina puxianensis n. sp. is described and illustrated from Shanxi province, China. It is characterized by having a triangular dorsal tooth, pointing towards the ventral side, anterior to two sub-ventral teeth, with dorsal and sub-ventral teeth similar in shape and size; one ventromedian seta in the cervical region; vulva with protruding lips, absence of post-uterine sac; and a pair of setae on the tail; male not found; female body length 1048–1331 μm, a = 25.5–33.8, b = 5.5–6.2, c = 14.4–23.1, c' = 2.1–3.5 and V = 56.1–68.5%. The phylogenetic relationships among species in the genus Tripylina were analyzed using data from the SSU (small subunit) and D2/D3 expansion segments of the LSU (large subunit) rDNA. These analyses confirmed that T. puxianensis n. sp. is different from other members of the genus for which sequences are available.

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