Influence of aminopenicillins on bacterial growth kinetics in vitro in comparison with the antibacterial effect in vivo

Infection ◽  
1979 ◽  
Vol 7 (S5) ◽  
pp. S434-S437 ◽  
Author(s):  
H. Mattie ◽  
G. B. van der Voet
Keyword(s):  
Growth Kinetics ◽  
Bacterial Growth ◽  
Antibacterial Effect

scholarly journals EFFECTS OF THE METHANOLIC EXTRACT FROM AROEIRA LEAVES (SCHINUS TEREBINTHIFOLIUS RADDI) ON THE IN VITRO GROWTH OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Luiz Erlon Araújo Rodrigues ◽  
Cláudia Valle Cabral Dias dos Santos ◽  
André Nascimento Santos
Keyword(s):  
Bacterial Growth ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Corynebacterium Pseudotuberculosis ◽  
In Vitro Growth ◽  
Caseous Lymphadenitis ◽  
Schinus Terebinthifolius ◽  
In Vivo Growth

Plants produce molecules such as terpenes, lectins and alkaloids that exert anticarcinogenic, antimicrobial and anti-inflammatory activities. Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in goats and sheep. The prevalence of this infection is high in northeastern of Brazil. The present study evaluated the effects of a methanolic extract from the leaves of the aroeira plant (Schinus terebinthifolius Raddi) on the in vivo growth of virulent and attenuated strains of this bacillus. The extract was tested at concentrations of 2000, 1500, 1000, 500, 250, 100 and 10 µg/dL for 48 hours. The first four concentrations inhibited bacterial growth, producing halos of decreasing diameters. No antibacterial effect was found with the remaining concentrations. These results show that bacterial growth was inhibited by an extract of Schinus terebinthifolius Raddi at the aforementioned concentrations.

scholarly journals Development of a Fluorescent Tool for Studying Legionella bozemanae Intracellular Infection

2021 ◽  
Vol 9 (2) ◽  
pp. 379
Author(s):  
Breanne M. Head ◽  
Christopher I. Graham ◽  
Teassa MacMartin ◽  
Yoav Keynan ◽  
Ann Karen C. Brassinga
Keyword(s):  
Growth Kinetics ◽  
Legionella Pneumophila ◽  
Fluorescent Protein ◽  
Disease Incidence ◽  
Acanthamoeba Castellanii ◽  
Infection Model ◽  
Intracellular Infection ◽  
Green Fluorescent

Legionnaires’ disease incidence is on the rise, with the majority of cases attributed to the intracellular pathogen, Legionella pneumophila. Nominally a parasite of protozoa, L. pneumophila can also infect alveolar macrophages when bacteria-laden aerosols enter the lungs of immunocompromised individuals. L. pneumophila pathogenesis has been well characterized; however, little is known about the >25 different Legionella spp. that can cause disease in humans. Here, we report for the first time a study demonstrating the intracellular infection of an L. bozemanae clinical isolate using approaches previously established for L. pneumophila investigations. Specifically, we report on the modification and use of a green fluorescent protein (GFP)-expressing plasmid as a tool to monitor the L. bozemanae presence in the Acanthamoeba castellanii protozoan infection model. As comparative controls, L. pneumophila strains were also transformed with the GFP-expressing plasmid. In vitro and in vivo growth kinetics of the Legionella parental and GFP-expressing strains were conducted followed by confocal microscopy. Results suggest that the metabolic burden imposed by GFP expression did not impact cell viability, as growth kinetics were similar between the GFP-expressing Legionella spp. and their parental strains. This study demonstrates that the use of a GFP-expressing plasmid can serve as a viable approach for investigating Legionella non-pneumophila spp. in real time.

scholarly journals Is There Potential for Repurposing Statins as Novel Antimicrobials?

2016 ◽  
Vol 60 (9) ◽  
pp. 5111-5121 ◽  
Author(s):  
Emma Hennessy ◽  
Claire Adams ◽  
F. Jerry Reen ◽  
Fergal O'Gara
Keyword(s):  
Serum Cholesterol ◽  
Bacterial Growth ◽  
Bacterial Infections ◽  
Statin Treatment ◽  
Therapeutic Agents ◽  
Pleiotropic Effects ◽  
Current Information ◽  
Potential Use

ABSTRACTStatins are members of a class of pharmaceutical widely used to reduce high levels of serum cholesterol. In addition, statins have so-called “pleiotropic effects,” which include inflammation reduction, immunomodulation, and antimicrobial effects. An increasing number of studies are emerging which detail the attenuation of bacterial growth andin vitroandin vivovirulence by statin treatment. In this review, we describe the current information available concerning the effects of statins on bacterial infections and provide insight regarding the potential use of these compounds as antimicrobial therapeutic agents.

Ag functionalized SnS2 with enhanced photothermal activity for safe and efficient wound disinfection

2021 ◽  
Author(s):  
Jun Yang ◽  
Lin Sun ◽  
Shuhan Hui ◽  
Peng Zhang ◽  
Jian Li ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Antibacterial Effect ◽  
Photothermal Conversion ◽  
New Type ◽  
Nir Laser

A new type of Ag@SnS2 was established with Ag decorated on SnS2 surface for improved photothermal conversion capability. Ag@SnS2 exhibited a robust in vitro antibacterial effect and in vivo wound disinfection under NIR laser irradiation.

Core@shell structured ceria@mesoporous silica nanoantibiotics restrain bacterial growth in vitro and in vivo

2021 ◽  
pp. 112607
Author(s):  
Didem Şen Karaman ◽  
Christa Kietz ◽  
Prakirth Govardhanam ◽  
Anna Slita ◽  
Alexandra Manea ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Bacterial Growth ◽  
Core Shell

An in vitro and in vivo antibacterial effect of different plant extracts on Enterococcus faecalis as intracanal medicament

2020 ◽  
Vol 12 (4) ◽  
pp. 362
Author(s):  
NawalA Al-Sabawi ◽  
AliM Al-Naimi ◽  
EmanM Yahya
Keyword(s):  
Enterococcus Faecalis ◽  
Plant Extracts ◽  
Antibacterial Effect

Effect of Tannins on the In Vitro Growth of Escherichia coli O157:H7 and In Vivo Growth of Generic Escherichia coli Excreted from Steers

2007 ◽  
Vol 70 (3) ◽  
pp. 543-550 ◽  
Author(s):  
BYENG R. MIN ◽  
WILLIAM E. PINCHAK ◽  
ROBIN C. ANDERSON ◽  
TODD R. CALLAWAY
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Bacterial Growth ◽  
Escherichia Coli O157 ◽  
Tannin Extract ◽  
Bacterial Growth Rate ◽  
Linear Effect ◽  
E Coli

The effect of commercially available chestnut and mimosa tannins in vitro (experiment 1) or in vivo (experiment 2) on the growth or recovery of Escherichia coli O157:H7 or generic fecal E. coli was evaluated. In experiment 1, the mean growth rate of E. coli O157:H7, determined via the measurement of optical density at 600 nm during anaerobic culture in tryptic soy broth at 37°C, was reduced (P < 0.05) with as little as 400 μg of either tannin extract per ml of culture fluid. The addition of 200, 400, 600, 800, and 1,200 μg of tannins per ml significantly (P < 0.01) reduced the specific bacterial growth rate when compared with the nontannin control. The specific growth rate decreased with increasing dose levels up to 800 μg of tannins per ml. Bacterial growth inhibition effects in chestnut tannins were less pronounced than in mimosa tannins. Chestnut tannin extract addition ranged from 0 to 1,200 μg/ml, and a linear effect (P < 0.05) was observed in cultures incubated for 6 h against the recovery of viable cells, determined via the plating of each strain onto MacConkey agar, of E. coli O157:H7 strains 933 and 86-24, but not against strain 6058. Similar tests with mimosa tannin extract showed a linear effect (P < 0.05) against the recovery of E. coli O157:H7 strain 933 only. The bactericidal effect observed in cultures incubated for 24 h with the tannin preparations was similar, although it was less than that observed from cultures incubated for 6 h. When chestnut tannins (15 g of tannins per day) were infused intraruminally to steers fed a Bermuda grass hay diet in experiment 2, fecal E. coli shedding was lower on days 3 (P < 0.03), 12 (P = 0.08), and 15 (P < 0.001) when compared with animals that were fed a similar diet without tannin supplementation. It was concluded that dietary levels and sources of tannins potentially reduce the shedding of E. coli from the gastrointestinal tract.

scholarly journals Microbial Growth Inhibition by Alternating Electric Fields in Mice with Pseudomonas aeruginosa Lung Infection

2010 ◽  
Vol 54 (8) ◽  
pp. 3212-3218 ◽  
Author(s):  
Moshe Giladi ◽  
Yaara Porat ◽  
Alexandra Blatt ◽  
Esther Shmueli ◽  
Yoram Wasserman ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Inhibition ◽  
Electric Fields ◽  
Bacterial Growth ◽  
Lung Infection ◽  
Alternating Electric Fields ◽  
Microbial Growth Inhibition ◽  
Bacterial Growth Inhibition

ABSTRACT High-frequency, low-intensity electric fields generated by insulated electrodes have previously been shown to inhibit bacterial growth in vitro. In the present study, we tested the effect of these antimicrobial fields (AMFields) on the development of lung infection caused by Pseudomonas aeruginosa in mice. We demonstrate that AMFields (10 MHz) significantly inhibit bacterial growth in vivo, both as a stand-alone treatment and in combination with ceftazidime. In addition, we show that peripheral (skin) heating of about 2°C can contribute to bacterial growth inhibition in the lungs of mice. We suggest that the combination of alternating electric fields, together with the heat produced during their application, may serve as a novel antibacterial treatment modality.

scholarly journals Binding to and antibacterial effect of ampicillin, neomycin and polymyxin B on human faeces

1984 ◽  
Vol 93 (1) ◽  
pp. 27-34 ◽  
Author(s):  
M. P. Hazenberg ◽  
A. M. Pennock-Schröder ◽  
M. Van Den Boom ◽  
J. P. Van De Merwe
Keyword(s):  
Antibacterial Effect ◽  
Polymyxin B ◽  
Selective Decontamination ◽  
Faecal Flora ◽  
Selective Effect ◽  
Human Faeces ◽  
Obligate Anaerobic ◽  
Human Flora

SUMMARYNeomycin and polymyxin B, used during selective decontamination of the gastrointestinal tract, were studied for their effect on the human faecal florain vitro. The selective effect was found to be associated with a relative insusceptibility of the obligate anaerobic flora as compared with the facultatively anaerobic Gram-negative rods (Escherichia coli). Both neomycin and polymyxin B were bound by human faeces, in contrast to ampicillin. The results may explain the selective effect of neomycin and polymyxin B on the human florain vivo.

scholarly journals Dopamine Is a Siderophore-Like Iron Chelator That PromotesSalmonella entericaSerovar Typhimurium Virulence in Mice

mBio ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Stefanie Dichtl ◽  
Egon Demetz ◽  
David Haschka ◽  
Piotr Tymoszuk ◽  
Verena Petzer ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Iron Uptake ◽  
Iron Homeostasis ◽  
Iron Acquisition ◽  
Lipocalin 2 ◽  
Intracellular Iron ◽  
Content Type ◽  
Hepcidin Expression

ABSTRACTWe have recently shown that the catecholamine dopamine regulates cellular iron homeostasis in macrophages. As iron is an essential nutrient for microbes, and intracellular iron availability affects the growth of intracellular bacteria, we studied whether dopamine administration impacts the course ofSalmonellainfections. Dopamine was found to promote the growth ofSalmonellaboth in culture and within bone marrow-derived macrophages, which was dependent on increased bacterial iron acquisition. Dopamine administration to mice infected withSalmonella entericaserovar Typhimurium resulted in significantly increased bacterial burdens in liver and spleen, as well as reduced survival. The promotion of bacterial growth by dopamine was independent of the siderophore-binding host peptide lipocalin-2. Rather, dopamine enhancement of iron uptake requires both the histidine sensor kinase QseC and bacterial iron transporters, in particular SitABCD, and may also involve the increased expression of bacterial iron uptake genes. Deletion or pharmacological blockade of QseC reduced but did not abolish the growth-promoting effects of dopamine. Dopamine also modulated systemic iron homeostasis by increasing hepcidin expression and depleting macrophages of the iron exporter ferroportin, which enhanced intracellular bacterial growth.Salmonellalacking all central iron uptake pathways failed to benefit from dopamine treatment. These observations are potentially relevant to critically ill patients, in whom the pharmacological administration of catecholamines to improve circulatory performance may exacerbate the course of infection with siderophilic bacteria.IMPORTANCEHere we show that dopamine increases bacterial iron incorporation and promotesSalmonellaTyphimurium growth bothin vitroandin vivo. These observations suggest the potential hazards of pharmacological catecholamine administration in patients with bacterial sepsis but also suggest that the inhibition of bacterial iron acquisition might provide a useful approach to antimicrobial therapy.

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