Footpaths of the Late-Soviet Environmental Turn: The “Forest City” of Novosibirsk’s Akademgorodok as a Sociotechnical Imaginary

The founding of Akademgorodok near Novosibirsk in the late 1950s features prominently in the historiography of the Thaw and the general turn of Soviet science to the eastern parts of the country. This article puts this story into the context of the formation of modern “green” ideas in the late Soviet Union and reconsiders the relationship between humans and nature, along with the definition of nature itself. Akademgorodok produced a telling visual perspective: the architectural plan for the city dictated that its scientific, industrial, and living zones were drowned deep in the taiga. Architects named this type of urban planning “diffusive,” and memoirists described it as a “Forest City.” Using the term of Sheila Jasanoff, we designate this “Forest City” as a sociotechnical imaginary of Akademgorodok. Our aim is to study the historical roots of the “Forest City” and how it became a collective imaginary. How did it happen that in the 1950s and 1960s, when the “faces” of Soviet cities were defined by districts of standard panel houses, that a city was built near Novosibirsk in which so much attention was given to pre-human flora, fauna, and landscapes? What ideas and intellectual contexts composed the concept of Akademgorodok as a “Forest City”? Our answer possesses two dimensions. First, the rejection of the use of decorative elements in housing construction in the post-Stalin epoch stimulated architects to pay more attention to the greening of cities. They revived the concept of a “garden city” proposed by Ebenezer Howard on a new level. Second, the evolution of the ideas of Mikhail Lavrentyev, the founder of Akademgorodok, who upon arrival in Siberia applied the productivist program manifested in the slogan “Siberia is a treasure of resources,” but later changed his opinion to more “green” views under the influence of the so-called “Baikal Discussion.” The viewpoints of Lavrentyev influenced the design of this “center” of Siberian science, and then he formulated the idea of a “Forest City.” These contexts enable the utopian horizons and the search for models of a constructed future that were typical of the Thaw era to reflect upon the important challenges of the contemporary Anthropocene.

Infections Independent of Contamination: From Organic Matter, Evolution or Stem Cells

This study presents evidence to propose that some human infections may derive Independent of contamination by invading pathogens. Diverse data suggest multiple pathways Independent of contamination may generate human infections. For instance, the first microorganisms that emerged from lifeless organic matter 3.6 billion years ago indicated transformation of lifeless organic matter to micro organisms. Viral infections do correspondent to a lifeless protein particle in a cell of a complex multi- cellular organism reproducing and spreading infections to other complex multi- cellular organisms. Some microbes -such as pseudomonas aeruginosa with a larger genome and greater functional complexity than common bacteria -may evolve from human flora as observed in mammalian decomposition in sterile soil. For, decomposer species are not foreign Invaders from the environment and they represent evolution of common microorganisms during mammalian decomposition. Human cells may produce microorganisms consistent with a proven genetic link between humans cells and the Christensenellaceae (a family in the phylum Firmicutes). Human stem cells which are capable to differentiate to epithelial cells and cancer and have the essentials to produce microbes are the most likely candidates to produce microorganisms. What may be almost certain and not experimentally validated is the possibility that infections have multiple pathways of origin independent of contamination. Most nosocomial and opportunistic infections may be endogenous. Our knowledge may demolish the dogma of contamination by foreign microbes as the exclusive source of infections and pave novel avenues to prevent and treat diverse infections.

Effect of Commercial Grade Methyl Parathion on the Survival of Pseudomonas at Varying Temperature and pH

The wide-spread use of methyl parathion as an agricultural insecticide has been causing an increased level of contamination in soil, vegetation and groundwater reservoirs and as well as adverse effects on human, flora, fauna and ecosystems. Therefore, eco-friendly and cost-effective bioremediation system are needed to remove these pollutants from the contaminated sites and mitigate its hazardous. For this purpose, a lab scale study was conducted to investigate the effect of high concentrations of commercial grade methyl parathion (50 EC) on growth of indigenous soil Pseudomonas IES-Ps-1 under different environmental conditions such as pH and temperature. To determine the tolerance limit, Pseudomonas IES-Ps-1 was grown in nutrient broth supplemented with methyl parathion (0, 200, 400, 600, 800, 1000 and 1200 mg/l). The detailed pH (6, 7, 9) and temperature (25, 30 and 35 oC) studies were conducted using higher methyl parathion (400 and 800 mg/l). No growth was observed at 1000 and 1200 mg/l of pesticide after 96 hrs of incubation compared to other concentrations of 200, 400, 600 and 800 mg/l. The maximum growth for both 400 and 800 mg/l of pesticide was observed at pH 7 and 30 °C. The maximum removal of total organic carbon and chemical oxygen demand for 400 mg/lof pesticide were found 50 and 52 % while those for 800 mg/l were 46 and 49 % respectively. Hence, this study concluded that indigenous soil bacterium Pseudomonas IES-Ps-1 could serve as an efficient candidate at 30 °C and pH 7 in development of a bioremediation system for the removal of toxic effects of methyl parathion like pesticides from contaminated sites.

Effect of a Humanized Diet Profile on Colonization Efficiency and Gut Microbial Diversity in Human Flora-Associated Mice

Human flora-associated (HFA) mouse models allow us to design interventions for human disease research to test specific hypotheses and explore the complex commensal microbiome while avoiding the ethical limitations of using humans as models to directly study intestinal flora diseases. However, few studies have investigated the effect of a humanized diet profile (coarse-feed diet; CFD) on colonization efficiency and gut microbial diversity in HFA mice. We tested the colonization efficiency and gut microbial diversity in germ-free Kunming (KM) mice fed a CFD or a purified feed diet (PFD) at 1, 2, and 4 weeks. Although the colonization efficiencies differed significantly (67.50–70.00% vs. 72.69–85.96%) in the HFA mice, the colonization efficiency of the PFD-fed HFA mice (85.96%) was significantly higher than that of the CFD-fed mice (69.61%) at 2 weeks. At 4 weeks, the colonization efficiency of the PFD-fed mice (72.69%) was comparable to that of the CFD-fed mice (70.00%). Additionally, the gut microbial diversity of the CFD-fed HFA mice was similar to that of a human fecal donor. Regarding the Kyoto Encyclopedia of Genes and Genomes colonic microbiota metabolic pathways, the CFD-fed HFA mice showed more similarities to the human donor than to the PFD-fed mice in amino sugar and nucleotide sugar metabolism, biosynthesis of amino acids, carbon metabolism, purine metabolism, and phosphotransferase systems. In conclusion, the humanized diet profiles of the CFD and PFD could help establish human microbiotas in mice. Constructing HFA mouse models fed a CFD for 4 weeks may be useful in researching human-derived intestinal diseases.

Of men in mice: the development and application of a humanized gnotobiotic mouse model for microbiome therapeutics

Considerable evidence points to the critical role of the gut microbiota in physiology and disease. The administration of live microbes as a therapeutic modality is increasingly being considered. However, key questions such as how to identify candidate microorganisms and which preclinical models are relevant to recapitulate human microbiota remain largely unanswered. The establishment of a humanized gnotobiotic mouse model through the fecal microbiota transplantation of human feces into germ-free mice provides an innovative and powerful tool to mimic the human microbial system. However, numerous considerations are required in designing such a model, as various elements, ranging from the factors pertaining to human donors to the mouse genetic background, affect how microbes colonize the gut. Thus, it is critical to match the murine context to that of human donors to provide a continuous and faithful progression of human flora in mice. This is of even greater importance when the need for accuracy and reproducibility across global research groups are taken into account. Here, we review the key factors that affect the formulation of a humanized mouse model representative of the human gut flora and propose several approaches as to how researchers can effectively design such models for clinical relevance.

MRSA/MSSA causing infections: prevalence of mecA gene

Introduction Staphylococcus aureus is part of the human flora, present in the skin and mucous membranes but can become pathogenic, causing a wide spectrum of infections that were initially treated with penicillin. However, were observed some strains with resistance to this antibiotic and, therefore was developed a new antibiotic, the methicillin. After its introduction, arose the first S. aureus with resistance to methicillin (MRSA) due to the presence of a gene known as mecA that encodes an altered penicillin binding protein (PBP2a). In Europe, it is estimated that MRSA are associated to 44% of hospital acquired infections and its mortality rate is around 20%. Objectives Prevalence of MRSA strains in different types of infection in Coimbra district. Methodology Were analysed a total of 539 isolates of S. aureus previously characterized to the antibiotic susceptibility profile in the Hospital and University Center of Coimbra. Through the minimum inhibitory concentration (MIC) of oxacillin we classified our strains into MRSA and S. aureus methicillin-sensitive (MSSA); simultaneously, the mecA gene was detected by Polymerase Chain Reaction (PCR). Results Of the 539 isolates, 49% were considered MRSA and 51% MSSA. All MRSA isolates express the mecA gene, but from the total of 276 MSSA, 191 show this gene but do not express it. MRSA isolates were mostly from respiratory tract samples (48%) and blood cultures (21%) while MSSA were isolated in skin and soft tissue samples (35%). Conclusion MRSA are considered one of the primary pathogens for the development of pneumonia and septicaemia due to its highly virulent potential and the increasing expression of genetic determinants of antimicrobial resistance. Therefore, infections caused by MRSA continue with highly representability in the clinical context and their dissemination is a public health problem.

Capnocytophaga bacteremia precipitating severe thrombocytopenia and preterm labor in an asplenic host

Capnocytophaga species are gram-negative bacilli that inhabit mammalian oral surfaces and can cause opportunistic infection, especially in asplenic patients. The species Capnocytophaga canimorsus is particularly associated with dog bites and is known to cause endocarditis, meningitis, and sepsis in the general population. In pregnant patients, infections tied to Capnocytophaga species from human flora have been associated with preterm labor, chorioamnionitis, and neonatal septicemia. There is little known about the effects of zoonotically-acquired Capnocytophaga infection in pregnant patients. In this case report, we present a patient with Capnocytophaga bacteremia acquired after a dog bite associated with profound thrombocytopenia and preterm labor. Dog bites are common in the United States, and we present basic recommendations for management of dog bites in pregnant patients in order to avoid morbidity associated with delay in time to antibiotic treatment of infection as described in this case.

A case report of necrotizing fasciitis with growth of Actinomyces europaeus and Actinotignum schaalii

Actinomyces europeaeus and Actinotignum schaalii are two facultative anaerobes that are common contaminants of human flora; namely the urinary tract, the female genital tract and the gastrointestinal tract. A. europeaeus has been linked with abscesses, decubitus ulcers and purulent urethritis, while A. schaalii has been associated with urinary tract infections, bacteremia and Fournier’s gangrene. Here we present a case report of an 84-year-old female patient found to have a necrotizing soft tissue infection caused by A. europeaeus and A. schaalii. To our knowledge, this is the first case report that documents A. europeaeus as a causal agent of a necrotizing infection.

Emergence of mcr-1 mediated colistin resistant Escherichia coli from a hospitalized patient in Bangladesh

Introduction: The emergence of plasmid mediated mcr in bacteria has become global public health threat. Herein, we report a mcr-1 positive E. coli in normal human flora from a patient admitted in Dhaka Medical College Hospital (DMCH). Methodology: In total, 700 non-duplicate rectal swabs were collected from DMCH during 13th May to 12th June 2018. E. coli from rectal swabs were isolated on chromogenic UTI media containing vancomycin 10mg/l (Liofilchem, Italy) and confirmed by MALDI-TOF. Minimum inhibitory concentrations (MIC) were determined by agar dilution and interpreted according to EUCAST breakpoints. Genomic analysis of mcr positive E. coli (MCRPEC) was performed by Illumina MiSeq sequencing and pulsed field gel electrophoresis (PFGE) using S1 nuclease DNA digests and blamcr-1 probing. Transferability of blamcr-1 were determined by conjugation assays. Results: We found one MCRPEC from 700 rectal swab screening which was isolated from the rectal swab culture of a 17-year boy who was admitted to the burns ICU, DMCH with 53% flame burn involving much of the trunk and face. Genome sequencing revealed that mcr-1 was present on an IncH12 plasmid of 257,243 bp and flanked by ISApaI1. The colistin resistance can be transferred to the recipient Klebsiella varricola with a frequency of 8.3 × 10-5. Transconjugants were more resistant to colistin than donor (MIC 32 µg/mL). Conclusions: This is the first human associated mcr in Bangladesh. These data indicate the need for a systematic “one health” surveillance in the country.