The relationship of lymphocytic thyroiditis to the development of thyroid carcinoma

Abstract Objective To investigate the relationship of BRAF mutation to the outcome of the first postoperative 131I treatment and malignant biological characteristics in papillary thyroid carcinoma (PTC) and the clinical value of circulating tumor DNA (ctDNA). Methods Thirty-three patients with PTC underwent the first 131I treatment after total thyroidectomy were enrolled in this study. BRAF mutation based on postoperative tumor tissue and ctDNA in peripheral blood before 131I treatment were detected. According to the status of BRAF mutation, all patients were divided into 2 groups for the categories of tumor tissues and ctDNA respectively: i) BRAF mutated, ii) BRAF wild-type. The Fisher's exact test was performed to analyze the relationship of BRAF mutation in either tumor tissue or ctDNA to the outcome of the first 131I treatment and malignant characteristics of PTC. Results BRAF mutation was detected in 25 patients based on tumor tissue (25/33,75.8%), and all the patients had single mutation site. In ctDNA, BRAF mutation was detected in 5 patients (5/33, 15.2%), and all the patients had single mutation site. BRAF mutation showed no relationship with the outcome of the first 131I treatment and the malignant biological characteristics in both tumor tissues and ctDNA(P>0.05). Conclusion The value of BRAF mutation alone might be limited in predicting therapeutic outcome of the first 131I treatment in PTC. No certain relevance was found between BRAF mutation and malignant biological features in PTC.

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Hubungan Daerah Tempat Tinggal dengan Gambaran Histopatologi Karsinoma Tiroid pada Masyarakat Sumatera Barat

Jurnal Kesehatan Andalas ◽

10.25077/jka.v3i2.65 ◽

2014 ◽

Vol 3 (2) ◽

Author(s):

Eka Putri ◽

Daan Khambri ◽

Selfi Renita Rusdji

Keyword(s):

At Risk ◽

Thyroid Carcinoma ◽

Iodine Deficiency ◽

P Value ◽

Residential Areas ◽

Chi Square ◽

Thyroid Carcinomas ◽

Not At Risk ◽

Relationship Of ◽

The Relationship

AbstrakKarsinoma tiroid merupakan keganasan kelenjar endokrin yang paling sering ditemukan dan insidennya meningkat setiap tahun. Berdasarkan gambaran histopatologinya, karsinoma tiroid dibagi menjadi tipe papiler, folikuler, meduler, dan anaplastik. Salah satu faktor yang mempengaruhi gambaran histopatologi karsinoma tiroid adalah keadaan geografis, dimana karsinoma tipe folikuler dan anaplastik akan lebih sering ditemukan pada daerah beresiko defisiensi yodium dibandingkan daerah tidak beresiko defisiensi yodium. Tujuan dari penelitian ini adalah untuk mengetahui hubungan daerah tempat tinggal dengan gambaran histopatologi karsinoma tiroid. Penelitian ini menggunakan desain analitik observasional dengan mengumpulkan data sekunder di Laboratorium Patologi Anatomi FK Unand dan rekam medik RSUP M. Djamil periode Januari 2010 – Desember 2011 Dari penelitian ini ditemukan 102 kasus karsinoma tiroid, dimana 32 kasus bertempat tinggal di daerah beresiko defisiensi yodium dan 70 kasus bertempat tinggal di daerah tidak beresiko defisiensi yodium. Pada daerah beresiko defisiensi yodium, 34,4% merupakan karsinoma folikuler dan anaplastik, dan 65,5% merupakan karsinoma selain folikuler dan anaplastik. Pada daerah tidak beresiko defisiensi yodium, 22,9% merupakan karsinoma folikuler dan anaplastik, dan 77,1% merupakan karsinoma selain folikuler dan anaplastik. Dari analisis statistik hubungan daerah beresiko defisiensi yodium dengan kejadian karsinoma folikuler dan anaplastik berdasarkan uji chi-square didapatkan nilai p =0,33. Tidak terdapat hubungan bermakna antara daerah tempat tinggal dengan gambaran histopatologi karsinoma tiroid pada masyarakat Sumatera Barat pada periode Januari 2010 – Desember 2011. Kata kunci: Gambaran histopatologi karsinoma tiroid, daerah tempat tinggalKata kunci: Gambaran histopatologi karsinoma tiroid, daerah tempat tinggalAbstractThyroid carcinoma is the most common endocrine tumor and is increasing in incidence every year. Based on histopathological types, thyroid carcinomas are divided into papillary carcinoma, follicular carcinoma, medullary carcinoma, and anaplastic carcinomas. Factors that can influence the histopathological type of thyroid carcinomas is geographical factor, which follicular and anaplastic type of thyroid carcinomas more frequently found in areas at risk of iodine deficiency than the area not at risk of iodine deficiency.The purpose of this study was to determine the relationship of residential areas with histopathologic types of thyroid carcinoma. This analytic research with observational design obtaining secondary data from Pathology Anatomy Laboratory of Medical Faculty of Andalas University and medical record of M. Djamil Hospital in a period of January 2010 – December 2011. From this reseach, found 102 cases of thyroid carcinoma, of which 32 cases residing in areas at risk of iodine deficiency and 70 cases residing in the area are not at risk of iodine deficiency. In areas at risk of iodine deficiency, 34.4% are follicular and anaplastic carcinomas, and 65.5% are papillary carcinomas. In areas not at risk of iodine deficiency, 22.9% are follicular and anaplastic carcinomas, and 77.1% are papillary carcinomas. Statistical analysis of the relationship of the area at risk of iodine deficiency with follicular and anaplastic carcinoma incidence by chi-square test p value = 0.32.There is no significant correlation between residential areas and histopathological types of thyroid carcinoma of West Sumatera society.Keywords:Histopathological types of thyroid carcinoma, residential areas

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MicroRNA-3666 Regulates Thyroid Carcinoma Cell Proliferation via MET

Cellular Physiology and Biochemistry ◽

10.1159/000443054 ◽

2016 ◽

Vol 38 (3) ◽

pp. 1030-1039 ◽

Cited By ~ 23

Author(s):

Gang Wang ◽

Chengzhong Cai ◽

Lei Chen

Keyword(s):

Cell Proliferation ◽

Cell Growth ◽

Thyroid Carcinoma ◽

Cell Apoptosis ◽

Luciferase Reporter ◽

Bioinformatics Analyses ◽

Survival And Growth ◽

Relationship Of ◽

The Relationship

Background/Aims: Thyroid carcinoma (TC) is a highly lethal malignant cancer and its carcinogenesis remains undetermined. Dysregulation of microRNAs (miRNAs) is well known to be involved in the development of various cancers, including TC, whereas a role of miR-3666 in the pathogenesis of TC has not been appreciated. Methods: We analyzed the levels of MET and miR-3666 in TC tissue and the relationship of miR-3666 levels with patients' prognosis. We then overexpressed miR-3666 by miRNA mimics transfection and inhibited miR-3666 by miRNA antisense transfection in TC cells. Cell survival and growth were analyzed by CCK-8 assay and MTT assay, respectively. Cell apoptosis and proliferation were analyzed by flow cytometry. Bioinformatics analyses were applied to predict miR-3666 targets, which was then confirmed using luciferase reporter assay. Results: We detected significantly higher levels of MET, and significantly lower levels of miR-3666 in TC tissue, compared to the adjacent non-tumor tissue. Moreover, the low miR-3666 levels were associated with poor survival of the patients. Overexpression of miR-3666 significantly inhibited cell growth, while depletion of miR-3666 increased cell growth in TC cells. Moreover, the effects of miR-3666 on cell growth appeared to result from alteration in cell proliferation, rather than changes in cell apoptosis. MiR-3666 was found to bind to the 3'-UTR of MET mRNA to inhibit its translation in TC cells. Conclusion: Reduced miR-3666 levels in TC tissue may promotes TC growth, possibly through MET-mediated cell proliferation.

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The relationship of the scleractinian corals to the rugose corals

Paleobiology ◽

10.1017/s0094837300025707 ◽

1980 ◽

Vol 6 (02) ◽

pp. 146-160 ◽

Cited By ~ 29

Author(s):

William A. Oliver

Keyword(s):

Critical Points ◽

Scleractinian Corals ◽

Convincing Evidence ◽

Early Triassic ◽

Rugose Corals ◽

History Of ◽

The Subject ◽

Relationship Of ◽

The Relationship ◽

Biologic Factors

The Mesozoic-Cenozoic coral Order Scleractinia has been suggested to have originated or evolved (1) by direct descent from the Paleozoic Order Rugosa or (2) by the development of a skeleton in members of one of the anemone groups that probably have existed throughout Phanerozoic time. In spite of much work on the subject, advocates of the direct descent hypothesis have failed to find convincing evidence of this relationship. Critical points are:(1) Rugosan septal insertion is serial; Scleractinian insertion is cyclic; no intermediate stages have been demonstrated. Apparent intermediates are Scleractinia having bilateral cyclic insertion or teratological Rugosa.(2) There is convincing evidence that the skeletons of many Rugosa were calcitic and none are known to be or to have been aragonitic. In contrast, the skeletons of all living Scleractinia are aragonitic and there is evidence that fossil Scleractinia were aragonitic also. The mineralogic difference is almost certainly due to intrinsic biologic factors.(3) No early Triassic corals of either group are known. This fact is not compelling (by itself) but is important in connection with points 1 and 2, because, given direct descent, both changes took place during this only stage in the history of the two groups in which there are no known corals.

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