Effect of temperature on the sugar content of potato callus tissue

1990 ◽  
Vol 67 (4) ◽  
pp. 209-215 ◽  
Author(s):  
Paul Muneta ◽  
S. Hagen ◽  
D. LeTourneau ◽  
J. Brown
1991 ◽  
Vol 68 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Steven R. Hagen ◽  
Paul Muneta ◽  
Duane LeTourneau ◽  
Janice Brown

Author(s):  
Talib Khashan Kareem ◽  
Abbas Tikki Karrar

This experiment was conducted in faculty of Science labs, Kufa University, carried out during 2015 to applied methods for extraction, purification and Quantitative of Lycopene red pigments, from callus tissue and tomato fruits mother plant (Lycopersicon esculentum Mill).This study include of three parts, Firstly; Tomato seeds(Supper queen) hybrid were germinated in free MS medium and callus induction from shoot tip (3cmpieces) by using MS medium supplemented with Dichlorophenoxiactic acid (2,4-D) at different concentration (0.5,1, 1.5mg/l)with benzyl adenine (BA) at concentration of (0.3 mg/l). Secondly; identically callus fresh weight re-cultured in the same MS medium supplemented with high molecular weight polyethylene glycol (PEG) was used as selective agent at level of (5,10,15 and 25%). Thirdly; comparisons study were made between in vitro and in vivo grown plant. Powder of control lycopene used as standard solution. The content of lycopene was done by using high performance liquid chromatography (HPLC), and compare of the quantitatively of lycopene with these content in fruits of mother plant, and callus tissue. Also, include alcohol extraction of Lycopene from tomato fruit by using acetone and hexane mixture. The result showed significant increased (P< 0.05) of lycopene production and the superiority of lycopene content in callus than the content in fruits of mother plant. Antioxidant enzymes activity like Catalase (CAT),Guaiacol peroxidise (POX) and Superoxide dismutase(SOD) were high in callus under drought stress than in fruit of mother plant. However, Proline and total sugar content were at higher levels in callus under drought stress than in fruit of mother plant.


2018 ◽  
Vol 6 (4) ◽  
pp. 307
Author(s):  
Ni Kadek Ariani Dewi ◽  
Amna Hartiati ◽  
Bambang Admadi Harsojuwono

The purpose of this reseach was 1) to determine the effect of temperature and acid type on hydrolysis of taro yam starch on the characteristics of glucose, 2) to determine the exact temperature and type of acid in taro starch hydrolysis which produced the best glucose characteristics. This reseach uses Randomized Block Design of factorial pattern.. The first factor is the hydrolysis temperature of taro yam starch at temperatures of 80°C, 90°C and 100°C. The second factor is the acids type of HNO3, H2SO4 and HCl, each with 7% acid concentration. Each treatment is grouped into 2 based on the implementation time. The variables observed were reduction in sugar content, total sugar, dekstrose equivalent (DE), total dissolved solids and clarity. The results showed that the temperature treatment and acid type had a very significant effect (P<0,01) on reducing sugar content, total sugar and hydrolyzed dectrose equivalent (DE) values. Temperature treatment has a significant effect on total dissolved solids and does not significantly affect clarity. The treatment of acid types has significant effect on total dissolved solids and clarity. Temperature treatment and type of acid interact with reducing sugar content, total sugar, and DE values, do not interact with total dissolved solids and clarity. The highest yield was obtained by reducing sugar content of 3,06%, total sugar 5,64% and DE value of 54,24% in HCl at 100°C. The total dissolved solids in H2SO4 and HCl at 100°C were 5,15°Brix and the clarity of HCl at 100°C was 0,02. The best glucose characteristics at HCl was 90°C which was not significantly different from HCl at 100°C. Keywords: sugar, hydrolysis, taro yam, starch, acid, glucose


The relation of the rate of respiration to the concentration of sugars in the cell is a problem of great theoretical importance. There are some indications in the literature of the effect of high external concentrations of sugar in increasing the respiration, but no full study of the relation of respiration to the internal sugar concentration has yet been published. For the analysis of the effect of changing internal sugar concentration the potato is a most suitable material, since its excess of hydrolysable starch allows one to alter the sugar content by exposure to cold and so produce a range of sugar between 0.2 per cent. and 7.0 per cent. at will. Moreover it respiration (Muller-Thurgau, 1882 ; Hopkins, 1924 ; Bennett and Bartholomew, 1924).


1993 ◽  
Vol 57 (8) ◽  
pp. 1311-1315 ◽  
Author(s):  
Heng-Long Wang ◽  
Ping-Du Lee ◽  
Rong-Huary Juang ◽  
Jong-Ching Su

2007 ◽  
Vol 67 (1) ◽  
pp. 127-136 ◽  
Author(s):  
Włodzimierz Krzesiński ◽  
Jerzy Stachowiak ◽  
Monika Gąsecka ◽  
Mikołaj Knaflewski

Sugar Content in Spears Versus Asparagus YieldingThis research was focused on determination of the correlation between soluble sugar content in green asparagus (Asparagus officinalisL.) spears and yield. In 2000-2002, green spears of the cultivar 'Thielim' were cut every day from the end of April to the middle of June. Sucrose, glucose, and fructose content was determined every 10-14 days by means of an HPLC method. The content of fructose was not correlated but the ratio of fructose to glucose content was positively correlated with the yield (r=0.92). When the ratio of fructose to glucose content in spears increased by 0.13, the yield increased by about 1 t·ha-1. Content of glucose, sucrose and soluble sugar were negatively correlated with yield (r = -0.76 r = -0.77 and -0.79, respectively). When glucose, sucrose and soluble sugar content in spears decreased by 0.29 mg·g-1, 0.20 mg·g-1and 0.85 mg·g-1, respectively, the yield increased by about 1 t·ha-1. During the first twenty days of harvest the calculated amount of soluble sugar accumulated in harvested spears was correlated with the yield in the remaining harvest period as well as in the whole harvest season. The effect of temperature and solar radiation on the yield and sugar content in spears is discussed in the paper.


2017 ◽  
Vol 15 (1) ◽  
pp. 24
Author(s):  
Marwati Marwati ◽  
Yuliani Yuliani ◽  
Yulian Andriyani ◽  
Mentari Mentari

Banana “kapas” is an original banana cultivar from Samarinda, with the characteristics at the time of mature is yellow fruit skin color, flesh color yellowish white and has a sweet taste. During this time the banana kapas has not been utilized properly. Based on the characteristics possessed by banana kapas can be processed into the processed products sale banana. This study aims to determine the effect of temperature and drying time on the chemical properties of sale of banana kapas produced, the level of panelist reception at organoleptic test and to get the time and temperature of the dryer that produces a quality banana sale based on chemical properties and organoleptic. The results showed that the temperature and duration of the dryer and its interactions had the significant effect on water content, ash content, vitamin C and reducing sugar content. Treatment with temperature 75oC and 21 hours drying time gave the best result with water content, ash content, vitamin C and reducing sugar are 32.80%, 1.13%, 60.65 mg/g and 16.08% respectively. For hedonic test, hedonic values of color, flavor, aroma and texture is like the hedonic quality of brown, sweet taste, rather banana flavored and hard texture.    


1970 ◽  
Vol 18 (2) ◽  
pp. 140-148
Author(s):  
A. Kleinendorst ◽  
R. Brouwer

Maize seedlings were grown in nutrient solution under 18-h daylength and the temperatures of the root medium and of the growing points of the leaves were varied independently between 20 degrees and 5 degrees C; leaf growth rate and leaf water content were continuously recorded. The effect of root temperature on leaf growth was influenced by the plant water balance and was subject to osmotic adaptation which proceeded faster and was more complete at favourable growth temperatures.-G.M.H. (Abstract retrieved from CAB Abstracts by CABI’s permission)


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