Salinity tolerance in Triticum aestivum-Lophopyrum elongatum amphiploid and 5E disomic addition line evaluated by NaCl effects on photosynthesis and respiration

1998 ◽  
Vol 26 (3) ◽  
pp. 281-287 ◽  
Author(s):  
Kazue Kasai ◽  
Hiroshi Fukayama ◽  
Naotsugu Uchida ◽  
Naoki Mori ◽  
Takeshi Yasuda ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salinity Tolerance ◽  
Addition Line ◽  
Disomic Addition Line ◽  
Lophopyrum Elongatum ◽  
Photosynthesis And Respiration

Characterization of alien chromosomes in backcross derivatives of Triticum aestivum × Elymus rectisetus hybrids by using molecular markers and sequential multicolor FISH/GISH

Genome ◽  
2012 ◽  
Vol 55 (5) ◽  
pp. 337-347 ◽  
Author(s):  
Quan-Wen Dou ◽  
Yunting Lei ◽  
Xiaomei Li ◽  
Ivan W. Mott ◽  
Richard R.-C. Wang
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Dna Sequences ◽  
Tan Spot ◽  
Stagonospora Nodorum ◽  
Gene Pools ◽  
Addition Line ◽  
Disomic Addition Line ◽  
Sts Markers

Wild Triticeae grasses serve as important gene pools for forage and cereal crops. Based on DNA sequences of genome-specific RAPD markers, sequence-tagged site (STS) markers specific for W and Y genomes have been obtained. Coupling with the use of genomic in situ hybridization, these STS markers enabled the identification of the W- and Y-genome chromosomes in backcross derivatives from hybrids of bread wheat Triticum aestivum L. (2n = 42; AABBDD) and Elymus rectisetus (Nees in Lehm.) Á. Löve & Connor (2n = 42; StStWWYY). The detection of six different alien chromosomes in five of these derivatives was ascertained by quantitative PCR of STS markers, simple sequence repeat markers, rDNA genes, and (or) multicolor florescence in situ hybridization. Disomic addition line 4687 (2n = 44) has the full complement of 42 wheat chromosomes and a pair of 1Y chromosomes that carry genes for resistance to tan spot (caused by Pyrenophora tritici-repentis (Died.) Drechs.) and Stagonospora nodorum blotch (caused by Stagonospora nodorum (Berk.) Castellani and Germano). The disomic addition line 4162 has a pair of 1St chromosomes and 21 pairs of wheat chromosomes. Lines 4319 and 5899 are two triple substitution lines (2n = 42) having the same chromosome composition, with 2A, 4B, and 6D of wheat substituted by one pair of W- and two pairs of St-genome chromosomes. Line 4434 is a substitution–addition line (2n = 44) that has the same W- and St-genome chromosomes substituting 2A, 4B, and 6D of wheat as in lines 4319 and 5899 but differs by having an additional pair of Y-genome chromosome, which is not the 1Y as in line 4687. The production and identification of these alien cytogenetic stocks may help locate and isolate genes for useful agronomic traits.

Characterization, identification and evaluation of a novel wheat-Agropyron cristatum (L.) Gaertn. disomic addition line II-30-5

2020 ◽  
Vol 67 (8) ◽  
pp. 2213-2223 ◽  
Author(s):  
Qingfeng Li ◽  
Yuqing Lu ◽  
Cuili Pan ◽  
Zhangjun Wang ◽  
Fenglou Liu ◽  
...  
Keyword(s):  
Agropyron Cristatum ◽  
Addition Line ◽  
Disomic Addition Line

scholarly journals Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165957 ◽  
Author(s):  
Qingfeng Li ◽  
Yuqing Lu ◽  
Cuili Pan ◽  
Miaomiao Yao ◽  
Jinpeng Zhang ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Agronomic Traits ◽  
Agropyron Cristatum ◽  
Addition Line ◽  
Disomic Addition Line

Field Screening of Wheat (Triticum aestivum L.) Genotypes for Salinity Tolerance at Three Locations in Egypt

2015 ◽  
Vol 4 (3) ◽  
pp. 88-104 ◽  
Author(s):  
A. Al-Naggar ◽  
S. Sabry ◽  
M. Atta ◽  
Ola El-Aleem
Keyword(s):  
Triticum Aestivum ◽  
Salinity Tolerance ◽  
Triticum Aestivum L ◽  
Field Screening

scholarly journals CARBON DIOXIDE EXCHANGE OF A WHEAT STAND GROWN IN NASA'S BIOMASS PRODUCTION CHAMBER

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1151d-1151
Author(s):  
K.A. Corey ◽  
R.M. Wheeler ◽  
J.C. Sager ◽  
R.P. Prince
Keyword(s):  
Carbon Dioxide ◽  
Triticum Aestivum ◽  
Biomass Production ◽  
Mass Flow ◽  
Compensation Point ◽  
Carbon Dioxide Exchange ◽  
Film Culture ◽  
Short Term ◽  
Photosynthesis And Respiration

A wheat (Triticum aestivum cv. Yecora Rojo) stand was grown using nutrient film culture in the closed conditions of NASA's Biomass Production Chamber. Rates of photosynthesis and respiration of the entire stand (about 20 m2) were determined daily using a regime of 20 hr light/4 hr dark, 20 C light/16 C dark an average PPF of 600 μmol/m2/s from HPS lamps, and a CO2 cone of 1000 ppm. Fractional interception of PPF by the stand reached a maximum of 0.96 at 24 days from planting. Rates of photosynthesis were constant throughout the photoperiod as determined by short term drawdowns of CO2 throughout the photoperiod. Drawdown rates of CO2 were correlated with rates determined by logging of mass flow of CO2 injected during chamber closure. Photosynthetic drawdowns of CO2 indicated that photosynthesis was not saturated at 1000 ppm CO2 and that the CO2 compensation point was about 50 ppm. Whole stand light compensation points were 200 to 250 μmol/m2/s between days 13 and 70 and then increased rapidly during senescence.

scholarly journals Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes

2020 ◽  
Vol 21 (11) ◽  
pp. 4053
Author(s):  
Liqiang Song ◽  
Hui Zhao ◽  
Zhi Zhang ◽  
Shuai Zhang ◽  
Jiajia Liu ◽  
...  
Keyword(s):  
Addition Line ◽  
Disomic Addition Line ◽  
Molecular Cytogenetic ◽  
Exploitation And Utilization ◽  
Specific Locus ◽  
Domestication Process ◽  
Disomic Addition Lines ◽  
M Genome ◽  
Black Color

Production of wheat-alien disomic addition lines is of great value to the exploitation and utilization of elite genes originated from related species to wheat. In this study, a novel wheat-Aegilops biuncialis 5Mb disomic addition line WA317 was characterized by in situ hybridization (ISH) and specific-locus amplified fragment sequencing (SLAF-seq) markers. Compared to its parent Chinese Spring (CS), the glumes of WA317 had black color and were difficult to remove after harvesting, suggesting chromosome 5Mb carried gene(s) related to glume development and Triticeae domestication process. A total of 242 Ae. biuncialis SLAF-based markers (298 amplified patterns) were developed and further divided into four categories by Ae. biuncialis Y17, Ae. umbellulata Y139 and Ae. comosa Y258, including 172 markers amplifying the same bands of U and M genome, six and 102 markers amplifying U-specific and M-specific bands, respectively and eighteen markers amplifying specific bands in Y17. Among them, 45 markers had the specific amplifications in WA317 and were 5Mb specific markers. Taken together, line WA317 with tenacious and black glumes should serve as the foundation for understanding of the Triticeae domestication process and further exploitation of primitive alleles for wheat improvement. Ae. biuncialis SLAF-based markers can be used for studying syntenic relationships between U and M genomes as well as rapid tracking of U and M chromosomal segments in wheat background.

scholarly journals Screening of recombinant inbred lines for salinity tolerance in bread wheat (Triticum aestivum L.)

2011 ◽  
Vol 10 (60) ◽  
pp. 12875-12881 ◽  
Author(s):  
Azadi Amin ◽  
Majidi Hervan Eslam ◽  
Abolghasem Mohammadi Seyed ◽  
Moradi Foad ◽  
Nakhoda Babak ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Salinity Tolerance ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Triticum Aestivum L ◽  
Inbred Lines

Isolation and characterization of a wheat – Psathyrostachys huashanica ‘Keng’ 3Ns disomic addition line with resistance to stripe rust

Genome ◽  
2014 ◽  
Vol 57 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Wanli Du ◽  
Jing Wang ◽  
Yuhui Pang ◽  
Liangming Wang ◽  
Jun Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Agronomic Traits ◽  
Addition Line ◽  
Homoeologous Group ◽  
Psathyrostachys Huashanica ◽  
Disomic Addition Line ◽  
Multiple Loci ◽  
Isolation And Characterization ◽  
Group 3 ◽  
Alien Chromatin

We isolated a wheat germplasm line, 22-2, which was derived from common wheat (Triticum aestivum ‘7182’) and Psathyrostachys huashanica ‘Keng’ (2n = 2x = 14, NsNs). Genomic composition and homoeologous relationships of 22-2 was analyzed using cytology, genomic in situ hybridization (GISH), EST–SSR, and EST–STS to characterize the alien chromatin in the transfer line. The cytological investigations showed that the chromosome number and configuration were 2n = 44 = 22 II. Mitotic and meiotic GISH using P. huashanica genomic DNA as the probe indicated that 22-2 contained a pair of P. huashanica chromosomes. The genomic affinities of the introduced P. huashanica chromosomes were determined by EST–SSR and EST–STS using multiple-loci markers from seven wheat homoeologous groups between the parents and addition line. One EST–SSR and 17 EST–STS markers, which were located on the homoeologous group 3 chromosomes of wheat, amplified polymorphic bands in 22-2 that were unique to P. huashanica. Thus, these markers suggested that the introduced Ns chromosome pair belonged to homoeologous group 3, so we designated 22-2 as a 3Ns disomic addition line. Based on disease reaction to mixed races (CYR31, CYR32, and Shuiyuan14) of stripe rust in the adult stages, 22-2 was found to have high resistance to stripe rust, which was possibly derived from its P. huashanica parent. Consequently, the new disomic addition line 22-2 could be a valuable donor source for wheat improvement depending on the excellent agronomic traits, especially, the introduction of novel disease resistance genes into wheat during breeding programs.

Attempts to induce homoeologous pairing between wheat and Agropyron cristatum genomes

Genome ◽  
2006 ◽  
Vol 49 (2) ◽  
pp. 190-193 ◽  
Author(s):  
Mélanie Jubault ◽  
Anne-Marie Tanguy ◽  
Paulette Abélard ◽  
Olivier Coriton ◽  
Jean-Claude Dusautoir ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Major Gene ◽  
Wild Relatives ◽  
Agropyron Cristatum ◽  
Second Step ◽  
Addition Line ◽  
Homoeologous Pairing ◽  
Ph1 Gene ◽  
Alien Genes ◽  
Suitable Technique

Agropyron cristatum (2n = 4x = 28, PPPP) possesses potentially valuable traits that could be used in wheat (Triticum aestivum) improvement through interspecific hybridization. Homoeologous pairing between wheat chromosomes and P chromosomes added to wheat in a set of wheat – A. cristatum addition lines was assessed. First, the Ph-suppressing effect of P chromosomes (except 7P) was analyzed. It was concluded that this system is polygenic with no major gene, and consequently, has no prospect in the transfer of alien genes from wild relatives. In a second step, the potential of the deletion ph1b of the Ph1 gene for inducing P–ABD pairing was evaluated. Allosyndetic associations between P and ABD genomes are very rare. This very low level of pairing is likely due to divergence in the repeated sequences between Agropyron and wheat genomes. Development of translocation lines using ionizing radiation seems to be a more suitable technique than homoeologous recombination to exploit the A. cristatum genome in wheat improvement.Key words: Triticum aestivum, Agropyron cristatum, addition line, GISH, Ph1 gene.

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