Characterization, identification and evaluation of a novel wheat-Agropyron cristatum (L.) Gaertn. disomic addition line II-30-5

Production of wheat-alien disomic addition lines is of great value to the exploitation and utilization of elite genes originated from related species to wheat. In this study, a novel wheat-Aegilops biuncialis 5Mb disomic addition line WA317 was characterized by in situ hybridization (ISH) and specific-locus amplified fragment sequencing (SLAF-seq) markers. Compared to its parent Chinese Spring (CS), the glumes of WA317 had black color and were difficult to remove after harvesting, suggesting chromosome 5Mb carried gene(s) related to glume development and Triticeae domestication process. A total of 242 Ae. biuncialis SLAF-based markers (298 amplified patterns) were developed and further divided into four categories by Ae. biuncialis Y17, Ae. umbellulata Y139 and Ae. comosa Y258, including 172 markers amplifying the same bands of U and M genome, six and 102 markers amplifying U-specific and M-specific bands, respectively and eighteen markers amplifying specific bands in Y17. Among them, 45 markers had the specific amplifications in WA317 and were 5Mb specific markers. Taken together, line WA317 with tenacious and black glumes should serve as the foundation for understanding of the Triticeae domestication process and further exploitation of primitive alleles for wheat improvement. Ae. biuncialis SLAF-based markers can be used for studying syntenic relationships between U and M genomes as well as rapid tracking of U and M chromosomal segments in wheat background.

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Isolation and characterization of a wheat – Psathyrostachys huashanica ‘Keng’ 3Ns disomic addition line with resistance to stripe rust

Genome ◽

10.1139/gen-2013-0199 ◽

2014 ◽

Vol 57 (1) ◽

pp. 37-44 ◽

Cited By ~ 12

Author(s):

Wanli Du ◽

Jing Wang ◽

Yuhui Pang ◽

Liangming Wang ◽

Jun Wu ◽

...

Keyword(s):

Stripe Rust ◽

Agronomic Traits ◽

Addition Line ◽

Homoeologous Group ◽

Psathyrostachys Huashanica ◽

Disomic Addition Line ◽

Multiple Loci ◽

Isolation And Characterization ◽

Group 3 ◽

Alien Chromatin

We isolated a wheat germplasm line, 22-2, which was derived from common wheat (Triticum aestivum ‘7182’) and Psathyrostachys huashanica ‘Keng’ (2n = 2x = 14, NsNs). Genomic composition and homoeologous relationships of 22-2 was analyzed using cytology, genomic in situ hybridization (GISH), EST–SSR, and EST–STS to characterize the alien chromatin in the transfer line. The cytological investigations showed that the chromosome number and configuration were 2n = 44 = 22 II. Mitotic and meiotic GISH using P. huashanica genomic DNA as the probe indicated that 22-2 contained a pair of P. huashanica chromosomes. The genomic affinities of the introduced P. huashanica chromosomes were determined by EST–SSR and EST–STS using multiple-loci markers from seven wheat homoeologous groups between the parents and addition line. One EST–SSR and 17 EST–STS markers, which were located on the homoeologous group 3 chromosomes of wheat, amplified polymorphic bands in 22-2 that were unique to P. huashanica. Thus, these markers suggested that the introduced Ns chromosome pair belonged to homoeologous group 3, so we designated 22-2 as a 3Ns disomic addition line. Based on disease reaction to mixed races (CYR31, CYR32, and Shuiyuan14) of stripe rust in the adult stages, 22-2 was found to have high resistance to stripe rust, which was possibly derived from its P. huashanica parent. Consequently, the new disomic addition line 22-2 could be a valuable donor source for wheat improvement depending on the excellent agronomic traits, especially, the introduction of novel disease resistance genes into wheat during breeding programs.

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Attempts to induce homoeologous pairing between wheat and Agropyron cristatum genomes

Genome ◽

10.1139/g05-074 ◽

2006 ◽

Vol 49 (2) ◽

pp. 190-193 ◽

Cited By ~ 15

Author(s):

Mélanie Jubault ◽

Anne-Marie Tanguy ◽

Paulette Abélard ◽

Olivier Coriton ◽

Jean-Claude Dusautoir ◽

...

Keyword(s):

Triticum Aestivum ◽

Major Gene ◽

Wild Relatives ◽

Agropyron Cristatum ◽

Second Step ◽

Addition Line ◽

Homoeologous Pairing ◽

Ph1 Gene ◽

Alien Genes ◽

Suitable Technique

Agropyron cristatum (2n = 4x = 28, PPPP) possesses potentially valuable traits that could be used in wheat (Triticum aestivum) improvement through interspecific hybridization. Homoeologous pairing between wheat chromosomes and P chromosomes added to wheat in a set of wheat – A. cristatum addition lines was assessed. First, the Ph-suppressing effect of P chromosomes (except 7P) was analyzed. It was concluded that this system is polygenic with no major gene, and consequently, has no prospect in the transfer of alien genes from wild relatives. In a second step, the potential of the deletion ph1b of the Ph1 gene for inducing P–ABD pairing was evaluated. Allosyndetic associations between P and ABD genomes are very rare. This very low level of pairing is likely due to divergence in the repeated sequences between Agropyron and wheat genomes. Development of translocation lines using ionizing radiation seems to be a more suitable technique than homoeologous recombination to exploit the A. cristatum genome in wheat improvement.Key words: Triticum aestivum, Agropyron cristatum, addition line, GISH, Ph1 gene.

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Detection of various U and M chromosomes in wheat-Aegilops biuncialis hybrids and derivatives using fluorescence in situ hybridisation and molecular markers

Czech Journal of Genetics and Plant Breeding ◽

10.17221/45/2012-cjgpb ◽

2012 ◽

Vol 48 (No. 4) ◽

pp. 169-177 ◽

Cited By ~ 6

Author(s):

A. Schneider ◽

M. Molnár-Láng

Keyword(s):

Ssr Markers ◽

Chromosome Pair ◽

In Situ Hybridisation ◽

Low Fertility ◽

Addition Line ◽

Fluorescence In Situ Hybridisation ◽

Addition Lines ◽

Disomic Addition Line ◽

Monosomic Addition ◽

Monosomic Addition Line

The aim of the study was to select wheat-Aegilops biuncialis addition lines carrying Aegilops biuncialis chromosomes differing from those which were introgressed into the wheat-Ae. biuncialis addition lines produced earlier in Martonvásár, Hungary. In the course of the experiments new wheat-Ae. biuncialis addition lines carrying chromosomes 2Ub, 6Mb, 6Ub; 5Ub, 3Ub, 7Ub; 5Mb, 6Mb and 7Mb were selected. The 2Ub disomic addition line is relatively stable, as 91% of the progenies contain this chromosome pair. The 6Mb disomic addition line proved to be dwarf and sterile, but it still exists as a monosomic addition line. Progenies analysed from the 6Ub monosomic addition line did not carry the 6Ub chromosome. One plant containing the 5Ub, 3Ub and 7Ub chromosomes and one plant carrying 5Mb, 6Mb and 7Mb chromosomes showed very low fertility. Each of the plants produced a single seed, but seeds of the parent plants are still available. Line No. 49/00 carried a submetacentric Ae. biuncialis chromosome pair and the chromosome number 44 has been constant for several generations. After FISH no hybridisation site was observed on the Ae. biuncialis chromosome pair using the pSc119.2 and Afa family repetitive DNA probes, so it was not possible to identify the Ae. biuncialis chromosome pair. However, the use of wheat SSR markers and the (GAA)n microsatellite DNA probe allowed it to be characterised more accurately. These new lines facilitate gene transfer from Ae. biuncialis into cultivated wheat and the selection of U and M genome-specific wheat SSR markers.

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A Cross between Bread Wheat and a 2D(2R) Disomic Substitution Triticale Line Leads to the Formation of a Novel Disomic Addition Line and Provides Information of the Role of Rye Secalins on Breadmaking Characteristics

10.20944/preprints202010.0284.v1 ◽

2020 ◽

Author(s):

Francesco Sestili ◽

Benedetta Margiotta ◽

Patrizia Vaccino ◽

Salvatore Moscaritolo ◽

Debora Giorgi ◽

...

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Bread Wheat ◽

Wheat Chromosome ◽

Addition Line ◽

Disomic Addition Line ◽

Triticale Line ◽

Cytogenetic Analyses ◽

Chromosome 1R

A bread wheat line (N11) and a disomic 2D(2R) substitution triticale line have been crossed and backrossed four times. At each step electrophoretic selection for the seeds that possessed, simultaneously, the complete set of high molecular weight glutenin subunits of N11 and the two high molecular weight secalins of rye, present in the 2D(2R) line, was carried out. Molecular cytogenetic analyses of the BC4F5 generation have revealed that the selection carried out had produced a disomic addition line (2n=44). The pair of additional chromosomes consisted of the long arm of chromosome 1R (1RL) from rye fused with the satellite body of the wheat chromosome 6B. Rheological analyses revealed that the dough obtained by the new addition line had higher quality characteristics when compared with the two parents. The role of the two additional high molecular weight secalins, present in the disomic addition line, in influencing improved dough characteristics is discussed.

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Salt tolerance of two wheat – Agropyron junceum disomic addition lines

Genome ◽

10.1139/g88-094 ◽

1988 ◽

Vol 30 (4) ◽

pp. 559-564 ◽

Cited By ~ 55

Author(s):

B. P. Forster ◽

T. E. Miller ◽

C. N. Law

Keyword(s):

Salt Tolerance ◽

Chinese Spring ◽

Major Gene ◽

Addition Line ◽

Similar Response ◽

Addition Lines ◽

Disomic Addition Line ◽

Wheat Group ◽

Group 2 ◽

Disomic Addition Lines

Two wheat – Agropyron junceum disomic addition lines homoeologous to groups 2 and 5 were tested for tolerance to salt. The experiments included germination and growth to maturity at various concentrations of sodium chloride (NaCl). The results were compared with those of wheat lines tetrasomic for chromosomes 2A, 2B, 2D, 5A, 5B, and 5D and also with the wheat parent 'Chinese Spring', and the salt-tolerant 'Chinese Spring' – A. junceum amphiploid. The addition of homoeologous group 2 chromosomes reduced the tolerance to salt relative to 'Chinese Spring' in every case. The order of tolerance was ranked as 'Chinese Spring' > 2J disomic addition line > tetra 2A = tetra 2D > tetra 2B. The addition of wheat group 5 chromosomes was either equal to 'Chinese Spring' or worse with respect to tolerance to salt. However, the disomic addition line for 5J showed considerable tolerance to salt and at 200 mol m−3 NaCl produced a similar response to that of the amphiploid in producing fertile tillers. Both produced viable grain, but the grain produced by the 5J addition line at 200 mol−3 NaCl was small and shrivelled, unlike the plump grain produced by the amphiploid. The order of tolerance was ranked as amphiploid > 5J addition line > 'Chinese Spring' = tetra 5A > tetra 5B = tetra 5D. It is concluded that there are genes on the group 2 chromosomes that confer susceptibility to salt and that chromosome 5J of A. junceum carries a major gene(s) for tolerance to salt. The potential for transferring this character into wheat is discussed. A hypothesis is also proposed to explain the function of the salt-tolerance gene(s) at critical stages in the life cycle of wheat.Key words: salt tolerance, wheat, Agropyron junceum, disomic addition lines.

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