Characterization of wheat curl mite resistance gene Cmc4 in OK05312

The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.

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Characterization of the Multi-Drug Resistance Gene cfr in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated From Animals and Humans in China

Frontiers in Microbiology ◽

10.3389/fmicb.2018.02925 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 7

Author(s):

Shu-Min Li ◽

Yu-Feng Zhou ◽

Liang Li ◽

Liang-Xing Fang ◽

Jia-Hong Duan ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Drug Resistance ◽

Resistance Gene ◽

Methicillin Resistant Staphylococcus Aureus ◽

Multi Drug Resistance ◽

Methicillin Resistant ◽

Drug Resistance Gene ◽

Mrsa Strains

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Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in AvianEscherichia coli

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.43.12.2925 ◽

1999 ◽

Vol 43 (12) ◽

pp. 2925-2929 ◽

Cited By ~ 165

Author(s):

Lydia Bass ◽

Cynthia A. Liebert ◽

Margie D. Lee ◽

Anne O. Summers ◽

David G. White ◽

...

Keyword(s):

Antibiotic Resistance ◽

Resistance Gene ◽

Multiple Drug Resistance ◽

Marker Gene ◽

Clinical Isolates ◽

Multiple Antibiotic Resistance ◽

Class 1 Integron ◽

E Coli ◽

Class 1

ABSTRACT Antibiotic resistance among avian bacterial isolates is common and is of great concern to the poultry industry. Approximately 36% (n = 100) of avian, pathogenic Escherichia coli isolates obtained from diseased poultry exhibited multiple-antibiotic resistance to tetracycline, oxytetracycline, streptomycin, sulfonamides, and gentamicin. Clinical avian E. coli isolates were further screened for the presence of markers for class 1 integrons, the integron recombinase intI1 and the quaternary ammonium resistance gene qacEΔ1, in order to determine the contribution of integrons to the observed multiple-antibiotic resistance phenotypes. Sixty-three percent of the clinical isolates were positive for the class 1 integron markersintI1 and qacEΔ1. PCR analysis with the conserved class 1 integron primers yielded amplicons of approximately 1 kb from E. coli isolates positive for intI1 andqacEΔ1. These PCR amplicons contained the spectinomycin-streptomycin resistance gene aadA1. Further characterization of the identified integrons revealed that many were part of the transposon Tn21, a genetic element that encodes both antibiotic resistance and heavy-metal resistance to mercuric compounds. Fifty percent of the clinical isolates positive for the integron marker gene intI1 as well as for theqacEΔ1 and aadA1 cassettes also contained the mercury reductase gene merA. The correlation between the presence of the merA gene with that of the integrase and antibiotic resistance genes suggests that these integrons are located in Tn21. The presence of these elements among avianE. coli isolates of diverse genetic makeup as well as inSalmonella suggests the mobility of Tn21 among pathogens in humans as well as poultry.

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