Analysis of the surface proteins of Acidithiobacillus ferrooxidans strain SP5/1 and the new, pyrite-oxidizing Acidithiobacillus isolate HV2/2, and their possible involvement in pyrite oxidation

2011 ◽  
Vol 193 (12) ◽  
pp. 867-882 ◽  
Author(s):  
Andreas Klingl ◽  
Christine Moissl-Eichinger ◽  
Gerhard Wanner ◽  
Josef Zweck ◽  
Harald Huber ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Surface Proteins

Biofilm Formation, Communication and Interactions of Mesophilic Leaching Bacteria during Pyrite Oxidation

2013 ◽  
Vol 825 ◽  
pp. 107-110
Author(s):  
Sören Bellenberg ◽  
Robert Barthen ◽  
Mario Vera ◽  
Nicolas Guiliani ◽  
Wolfgang Sand
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Pyrite Oxidation ◽  
Cell Communication ◽  
Homoserine Lactone ◽  
Mixed Cultures ◽  
Acyl Homoserine Lactone ◽  
Leaching Bacteria ◽  
Leaching Efficiency

A functional luxIR-type Quorum Sensing (QS) system is present in Acidithiobacillus ferrooxidans. However, cell-cell communication among various acidophilic chemolithoautotrophs growing on pyrite has not been studied in detail. These aspects are the scope of this study with emphasis on the effects exerted by the N-acyl-homoserine lactone (AHL) type signaling molecules which are produced by Acidithiobacillus ferrooxidans. Their effects on attachment and leaching efficiency by other leaching bacteria, such as Acidithiobacillus ferrivorans, Acidiferrobacter spp. SPIII/3 and Leptospirillum ferrooxidans in pure and mixed cultures growing on pyrite is shown.

Investigation of intermediate sulfur species during pyrite oxidation in the presence and absence of Acidithiobacillus ferrooxidans

2017 ◽  
Vol 167 ◽  
pp. 58-65 ◽  
Author(s):  
Zhihong Tu ◽  
Chuling Guo ◽  
Ting Zhang ◽  
Guining Lu ◽  
Jingjing Wan ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Sulfur Species ◽  
Presence And Absence

Pyrite Oxidation under initially neutral pH conditions and in the presence of <i>Acidithiobacillus ferrooxidans</i> and micromolar hydrogen peroxide

2012 ◽  
Vol 9 (1) ◽  
pp. 557-579 ◽  
Author(s):  
Y. Ma ◽  
C. Lin
Keyword(s):  
Hydrogen Peroxide ◽  
Acidithiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Surface Oxidation ◽  
Mineral Surfaces ◽  
Mineral Surface ◽  
Neutral Ph ◽  
Cell Adsorption ◽  
Ph Conditions ◽  
Marked Surface

Abstract. Hydrogen peroxide (H2O2) at a micromolar level played a role in the microbial surface oxidation of pyrite crystals under initially neutral pH. When the mineral-bacteria system was cyclically exposed to 50 μM H2O2, the colonization of \\textit{Acidithiobacillus ferrooxidans} onto the mineral surface was markedly enhanced, as compared to the control (no added H2O2). This can be attributed to the effects of H2O2 on increasing the roughness of the mineral surfaces, as well as the acidity and Fe2+ concentration at the mineral-solution interfaces. All of these effects tended to create more favourable nano- to micro-scale environments in the mineral surfaces for the cell adsorption. However, higher H2O2 levels inhibited the attachment of cells onto the mineral surfaces, possibly due to the oxidative stress in the bacteria when they approached the mineral surfaces where high levels of free radicals are present as a result of Fenton-like reactions. The more aggressive nature of H2O2 as an oxidant caused marked surface flaking of the mineral surface. The XPS results suggest that H2O2 accelerated the oxidation of pyrite-S and consequently facilitated the overall corrosion cycle of pyrite surfaces. This was accompanied by pH drop in the solution in contact with the pyrite cubes.

Interactions of Acidithiobacillus ferrooxidans with Heavy Metals, Various Forms of Arsenic and Pyrite

2007 ◽  
Vol 20-21 ◽  
pp. 423-426 ◽  
Author(s):  
Shailesh R. Dave ◽  
K.H. Gupta
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Ferrous Sulphate ◽  
Pyrite Oxidation ◽  
Environmental Parameters ◽  
Total Iron ◽  
Oxidation Study ◽  
Planktonic Bacteria ◽  
Presence And Absence ◽  
Ks Values

An arsenic resistant ferrous iron oxidizing bacterium Acidithiobacillus ferrooxidans (GenBank no. EF010878) was isolated from reactor leachate. The reactor leachate showed extreme environmental parameters. Ferrous iron concentrations of more than 60 g/L were found to be inhibitory in the presence and absence of arsenite. Ks values of 12.5 and 8.0 g/L ferrous sulphate and Vmax of 0.124 and 0.117 g/L/h/0.8 mg of protein were found in the presence and absence of arsenite respectively. At 14.9 g/L of arsenite and arsenate the culture showed 26.8 and 59.7 % ferrous iron oxidizing activity respectively. Amongst the metals studied, copper was found to be more toxic as compared to nickel and zinc. In the presence of 3.51 g/L nickel or 4.68 g/L zinc, about 30 % biooxidation activity was registered. In the pyrite oxidation study 87, 67 and 64 % of pyrite oxidation was found and 2.02, 3.19 and 5.96 g/L total iron was solubilized with 5, 10 and 20 g/L of pyrite respectively. The isolate was also able to oxidize refractory arsenopyrite gold ore and 0.531 g/L of arsenic was solubilized along with 0.872 g/L of soluble total iron. During this period the numbers of planktonic bacteria increased from 2.4 x 106 to 1.0 x 108 cells/mL.

Pyrite oxidation by Acidithiobacillus ferrooxidans at various concentrations of dissolved oxygen

2006 ◽  
Vol 225 (1-2) ◽  
pp. 16-29 ◽  
Author(s):  
Magdalena Gleisner ◽  
Roger B. Herbert ◽  
Paul C. Frogner Kockum
Keyword(s):  
Dissolved Oxygen ◽  
Acidithiobacillus Ferrooxidans ◽  
Pyrite Oxidation

The EPS of Acidithiobacillus ferrooxidans - a model for structure-function relationships of attached bacteria and their physiology

2001 ◽  
Vol 43 (6) ◽  
pp. 159-167 ◽  
Author(s):  
T. Gehrke ◽  
R. Hallmann ◽  
K. Kinzler ◽  
W. Sand
Keyword(s):  
Chemical Composition ◽  
Electrostatic Interaction ◽  
Acidithiobacillus Ferrooxidans ◽  
Extracellular Polymeric Substances ◽  
Pyrite Oxidation ◽  
Pyrite Surface ◽  
Hydrophobic Properties ◽  
Attached Bacteria ◽  
Primary Attachment ◽  
Leaching Bacteria

To dissolve pyrite or sulphur, leaching bacteria like Acidithiobacillus ferrooxidans attach to these substrata by extracellular polymeric substances (specifically, lipopolysaccharides). The primary attachment to pyrite at pH 2 is mediated by exopolymer-complexed iron(III) ions in an electrostatic interaction with the negatively charged pyrite surface. Cells grown on sulphur exhibit a different composition of the extracellular lipopolysaccharides, namely with increased hydrophobic properties, and do not attach to pyrite. Thus, the cells adapt the chemical composition of their exopolymers to the substrate/substratum. It is concluded that the mechanism of bacterial pyrite oxidation is basically indirect. The actual corrosive agents are iron(III) ions. Preliminary data indicate that active strains complex more iron(III) ions in their EPS than less active ones. Obviously, the exopolymeric layer comprises a reaction space for the regeneration of these ions by the activity of the iron oxidising bacteria.

Sulfur Product Layer in Sphalerite Biooxidation: Evidences for a Mechanism of Formation

2007 ◽  
Vol 20-21 ◽  
pp. 134-138 ◽  
Author(s):  
Dioni Mabel Zapata ◽  
M.A. Márquez ◽  
D.M.H. Ossa
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Elemental Sulfur ◽  
Pyrite Oxidation ◽  
Product Layer ◽  
Mechanism Of Formation ◽  
Unreacted Core ◽  
Mechanisms Of Formation ◽  
Iron And Zinc ◽  
Metal Bonds

The sphalerite-pyrite oxidation by Acidithiobacillus ferrooxidans was studied to analyze how the formation of the elemental sulfur layers occurs around sphalerite grains. Two possible mechanisms of formation have been raised. One mechanism corresponds to the formation of sulfur pseudomorphs where, both, iron and zinc have been leached of the sphalerite, whereas compound sulfur is oxidized, in situ, to elemental sulfur, leaving an unreacted core of sphalerite that remains in the center. Another mechanism consists in the dissolution of iron, zinc and sulfur presents. When the attack by Fe3+ as by H+ broken the S-metal bonds, sulfur is then oxidized to a series of sulfur intermediate compounds. These compounds in solution then are oxidized to elemental sulfur, which precipitates on sphalerite grains.

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