Fusarium wilt disease is a common disease for tomatoes plant which damages plant although it is intensively cultivated. Biological control by microbial antagonism is environmentally friendly compare to the use of fungicide. This research was aimed to study the capability of Bacillus subtilis, Pseudomonas fluorescens, Gliocladium fimbriatum and Trichoderma viridae that are locally specific in controll Fusarium oxysporum f.sp. lycopersici� cause tomato wilt disease. Evaluation of F.o. f.sp. lycopersici� antagonism capability was done by in vitro and in vivo.� In vitro antagonism treatment used the ouble plating treatment on Potato Dextose Agar.� In vivo antagonism treatment was done by gave antagonism agent and F.o. f.sp. lycopersici� together to the earth medium. The result of the study revealed that (1) B. subtilis, G. fimbriatum and T. viridae can pressure the growth and development �F.o. f.sp. lycopersici which cause tomato wilt disease both in vitro and in vivo, (2) In vitro the average of the prevented pathogen growth was as follow: B. subtilis (55.52%), G. fimbriatum (36.77%) and� T. viridae (35.87%), (3) In vivo treatment by the earth medium was not significantly different, and (4) In vivo the ability of prevent disease intensity was as follow : B. subtilis (62.35%), G. fimbriatum (49.95%) and� T. viridae (49.95%).