Characterization of a nanoflow sheath liquid interface and comparison to a sheath liquid and a sheathless porous-tip interface for CE-ESI-MS in positive and negative ionization

The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.

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Investigating the position of the separation capillary and emitter tube tips in a nanoflow sheath-liquid CE-ESI-MS interface to decouple the ESI potential

Talanta ◽

10.1016/j.talanta.2021.122212 ◽

2021 ◽

Vol 228 ◽

pp. 122212

Author(s):

Renáta Konášová ◽

Dušan Koval ◽

Jan Hošek ◽

Václav Kašička

Keyword(s):

Esi Ms ◽

Sheath Liquid

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Structural characterization of native and oxidized procyanidins (condensed tannins) from coffee pulp (Coffea arabica) using phloroglucinolysis and thioglycolysis-HPLC-ESI-MS

Food Chemistry ◽

10.1016/j.foodchem.2020.127830 ◽

2021 ◽

Vol 340 ◽

pp. 127830

Author(s):

Jorge E. Wong-Paz ◽

Sylvain Guyot ◽

Pedro Aguilar-Zárate ◽

Diana B. Muñiz-Márquez ◽

Juan C. Contreras-Esquivel ◽

...

Keyword(s):

Structural Characterization ◽

Coffea Arabica ◽

Condensed Tannins ◽

Coffee Pulp ◽

Esi Ms

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Determination of Ten Corticosteroids in Illegal Cosmetic Products by a Simple, Rapid, and High-Performance LC-MS/MS Method

International Journal of Analytical Chemistry ◽

10.1155/2017/3531649 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 7

Author(s):

Vita Giaccone ◽

Giuseppe Polizzotto ◽

Andrea Macaluso ◽

Gaetano Cammilleri ◽

Vincenzo Ferrantelli

Keyword(s):

High Performance ◽

Skin Diseases ◽

Gradient Elution ◽

Reversed Phase ◽

Cosmetic Products ◽

Chromatography Method ◽

Esi Ms ◽

Negative Ionization Mode ◽

Negative Ionization

The aim of our present work was the development of a rapid high-performance liquid chromatography method with electrospray ionization and tandem mass spectrometry detection (LC-ESI-MS/MS) for the determination of several corticosteroids in cosmetic products. Corticosteroids are suspected to be illegally added in cosmetic preparations in order to enhance the curative effect against some skin diseases. Sample preparation step consists in a single extraction with acetonitrile followed by centrifugation and filtration. The compounds were separated by reversed-phase chromatography with water and acetonitrile (both with 0.1% formic acid) gradient elution and detected by ESI-MS positive and negative ionization mode. The method was validated at the validation level of 0.1 mg kg−1. Linearity was studied in the 5–250 μg L−1 range and linear coefficients (r2) were all over 0.99. The accuracy and precision of the method were satisfactory. The LOD ranged from 0.085 to 0.109 mg kg−1 and the LOQ from 0.102 to 0.121 mg kg−1. Mean recoveries for all the analytes were within the range 91.9–99.2%. The developed method is sensitive and useful for detection, quantification, and confirmation of these corticosteroids in cosmetic preparations and can be applied in the analysis of the suspected samples under investigation.

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