The use of Li2O fortified growing compost to enhance lithiation in white Agaricus bisporus mushrooms: Li uptake and co-accumulation of other trace elements

AbstractIn an attempt to enrich the fruiting bodies with Lithium (Li), this study cultivated mushrooms using growing sets that were fortified with Li2O at 1.0, 5.0, 10, 50, 100 and 500 mg·kg−1 dw. Compost fortification up to 100 mg·kg−1 dw induced a dose-dependent increase in Li accumulation with resulting median mushroom concentrations of 2.0, 8.6, 16, 29 and 38 mg·kg−1 dw, respectively, relative to the unfortified control at 0.087 mg·kg−1 dw. The dose dependency appears to level off as Li2O addition approaches 100 mg·kg−1, suggesting that there is a limit to the ability of the species to accumulate/tolerate Li. Mushrooms did not grow at the 500 mg·kg−1 dw fortification level. At the highest viable level of fortification (100 mg·kg−1 dw), the fruiting bodies were around 440-fold richer in Li content than the control mushrooms. Additionally, the fortification at all levels up to 100 mg·kg−1 dw showed very low, if any, effect on the co-accumulation of the other, studied trace mineral constituents, with concentrations occurring at the lower range of those reported for commercial A. bisporus mushrooms.

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Lithiation of white button mushrooms (Agaricus bisporus) using lithium-fortified substrate: effect of fortification levels on Li uptake and on other trace elements

Environmental Science and Pollution Research ◽

10.1007/s11356-021-13984-6 ◽

2021 ◽

Author(s):

Sviatlana Pankavec ◽

Jerzy Falandysz ◽

Izabela Komorowicz ◽

Anetta Hanć ◽

Danuta Barałkiewicz ◽

...

Keyword(s):

Agaricus Bisporus ◽

Dry Weight ◽

Background Concentration ◽

Fruiting Bodies ◽

Button Mushrooms ◽

High Doses ◽

Dose Dependent Increase ◽

Dose Dependent ◽

White Button Mushrooms ◽

Li Content

AbstractHigh doses of lithium salts are used for the treatment or prevention of episodes of mania in bipolar disorder, but the medication is rapidly excreted and also shows side effects. Li may also be beneficial in people with mood disorders. Nutritionally, popular foods such as wild and cultivated mushrooms have low Li contents. This study evaluated the Li enrichment of white Agaricus bisporus mushrooms using Li2CO3 solutions to fortify the commercial growing substrate at various concentrations from 1.0 to 500 mg kg−1 dry weight (dw). Fortification of up to 100 mg kg−1 dw resulted in a significant (p < 0.01) dose-dependent increase in the accumulation of Li in mushroom, but the highest fortification level was found to be detrimental to fruitification. The median values of Li in fortified mushrooms corresponded to the fortification levels, increasing from 0.49 to 17 mg kg−1 dw relative to the background concentration of 0.056 mg kg−1 dw (control substrate contained 0.10 mg kg−1 dw). The potential for Li uptake in fruiting bodies was found to decrease at higher levels of fortification, with saturation occurring at 100 mg kg−1. Resulting lithiated mushrooms were up to 300-fold richer in Li content than specimens grown on control substrate. The fortification showed some effects on the uptake of other trace minerals, but concentrations of co-accumulated Ag, Al, As, Ba, Cd, Co, Cr, Cs, Cu, Hg, Mn, Ni, Pb, Rb, Sr, Tl, U, V and Zn were similar or lower than values reported in the literature for commercial A. bisporus. These lithiated mushrooms could be considered as a pro-medicinal alternative to treatments that use Li salts. Graphical abstract

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Arbutin Content and Tyrosinase Activity of Bergenia Extracts

Natural Product Communications ◽

10.1177/1934578x1701200422 ◽

2017 ◽

Vol 12 (4) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Lenka Tůmová ◽

Iva Dolečková ◽

Helena Hendrychová ◽

Marie Kašparová

Keyword(s):

Tyrosinase Activity ◽

The Other ◽

Aqueous Extracts ◽

Other Hand ◽

Ethanol Extracts ◽

Dose Dependent Increase ◽

Dose Dependent ◽

Diphenolase Activity

The total arbutin content in the leaves of all the studied Bergenia plants ( B. crassifolia, B. ciliata and B. x ornata) was determined. The highest values of the arbutin content have been established for B. crassifolia (58.9 ± 0.7 mg.g−1 DW) and B. x ornata (51.0 ± 1.21 mg.g−1 DW), and the lowest for B. ciliata (5.9 ± 0.6 mg.g−1 DW). Arbutin concentration in the Bergenia leaves was the lowest in spring, in the autumn, on the contrary it increased. All the tested aqueous extracts caused a dose-dependent increase in diphenolase activity of fungal tyrosinase in a similar way as arbutin. On the other hand, all the ethanol extracts inhibited the diphenolase activity of tyrosinase.

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Dose-Dependent Increase in Unconjugated Cinnamic Acid Concentration in Plasma Following Consumption of Polyphenol Rich Curry in the Polyspice Study

10.20944/preprints201806.0410.v1 ◽

2018 ◽

Author(s):

Sumanto Haldar ◽

Sze Han Lee ◽

Jun Jie Tan ◽

Siok Ching Chia ◽

Christiani Jeyakumar Henry ◽

...

Keyword(s):

Crossover Study ◽

Phenolic Acids ◽

Cinnamic Acid ◽

Phenylacetic Acid ◽

Plasma Concentrations ◽

The Other ◽

High Dose ◽

Dose Dependent Increase ◽

Dose Dependent ◽

Randomized Crossover Study

Spices rich in polyphenols are metabolized to a convergent group of phenolic acids. We conducted a dose-exposure nutrikinetic study to investigate associations between mixed spices intake and plasma concentrations of selected, unconjugated phenolic acids. In a randomized crossover study, 20 Chinese males consumed a curry meal containing 0 g, 6 g, and 12 g of mixed spices. Postprandial blood was drawn up to 7 h at regular intervals and plasma phenolic acids were quantified via LC-MS/MS. Cinnamic acid (CNA, p < 0.0001) and phenylacetic acid (PAA, p < 0.0005) concentrations were significantly increased with mixed spices consumption, although none of the other measured phenolic acids differ significantly between treatments. CNA displayed a high dose-exposure association (R2 > 0.8, p < 0.0001). The adjusted mean AUC0-7 h for CNA during the 3 increasing doses were 8.4 ± 3.4, 376.1 ± 104.7 and 875.7 ± 291.9 nM·h respectively. Plasma CNA concentration may be used as a biomarker of spice intake.

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Latent o-diphenol oxidase in mushrooms (Agaricus bisporus)

Canadian Journal of Biochemistry ◽

10.1139/o70-032 ◽

1970 ◽

Vol 48 (2) ◽

pp. 198-202 ◽

Cited By ~ 63

Author(s):

M. Yamaguchi ◽

P. M. Hwang ◽

J. D. Campbell

Keyword(s):

Agaricus Bisporus ◽

Sodium Lauryl Sulfate ◽

Soluble Fraction ◽

Lauryl Sulfate ◽

Fruiting Bodies ◽

Latent Form ◽

Freeze Dried ◽

Cultivated Mushrooms

An inactive or latent form of o-diphenol oxidase (EC 1.10.3.1) which can be activated by 0.1% sodium lauryl sulfate was found in cultivated mushrooms. The latent form was found in the soluble fraction of the fruiting bodies and constituted almost 95% of the total o-diphenol oxidase. Freeze-dried and acetone preparations gave the highest yields of the latent enzyme. There was more activity in the stalks than in the caps, and the activity increased with maturity. The white, golden cream, and sobexas strains of mushroom contained the latent form of the enzyme.

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Factors Influencing the Agaritine Content in Cultivated Mushrooms, Agaricus bisporus

Journal of Food Protection ◽

10.4315/0362-028x-46.6.506 ◽

1983 ◽

Vol 46 (6) ◽

pp. 506-509 ◽

Cited By ~ 8

Author(s):

J. J. SPERONI ◽

R. B. BEELMAN ◽

L. C. SCHISLER

Keyword(s):

Agaricus Bisporus ◽

Liquid Culture ◽

Fruiting Bodies ◽

Factors Influencing ◽

Commercially Important ◽

Cultivated Mushrooms

Agaritine concentrations were determined in fresh mushrooms grown from various spawn strains on several compost types and harvested at different phases of the cropping cycle. A wild spawn strain produced mushrooms with approximately two times the agaritine content of seven other more commercially important types. Mushrooms harvested from a synthetic compost produced significantly higher amounts of agaritine than five other compost types. Additionally, mushrooms harvested later in the cropping cycle were more likely to have higher agaritine levels compared to earlier harvested mushrooms. Agaritine was also present in the mycelium of Agaricus bisporus growing in liquid culture, but at much lower levels than present in the fruiting bodies.

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Endocrine activities of alexamorelin (Ala-His-d-2-methyl-Trp-Ala-Trp-d-Phe-Lys-NH2), a synthetic GH secretagogue, in humans

Acta Endocrinologica ◽

10.1530/eje.0.1430419 ◽

2000 ◽

pp. 419-425 ◽

Cited By ~ 6

Author(s):

F Broglio ◽

A Benso ◽

C Gottero ◽

G Muccioli ◽

R Deghenghi ◽

...

Keyword(s):

Young Adults ◽

The Other ◽

Endocrine Activity ◽

Gh Secretagogues ◽

Cortisol Responses ◽

Dose Dependent Increase ◽

Dose Dependent ◽

Synthetic Molecule ◽

Pituitary Receptors

OBJECTIVE: Peptidyl and non-peptidyl synthetic GH secretagogues (GHS) possess significant GH-, prolactin (PRL)- and ACTH/cortisol-releasing activity after i.v. and even p.o. administration, acting via specific hypothalamo-pituitary receptors in both animals and humans. The hexapeptide hexarelin (HEX) is a paradigmatic GHS whose activities have been widely studied in humans. The heptapeptide Ala-His-d-2-methyl-Trp-Ala-Trp-d-Phe-Lys-NH(2) (alexamorelin, ALEX) is a new synthetic molecule which inhibits GHS binding in vitro, but its endocrine activity has never been studied in humans. DESIGN: In six young adults we studied the effects of 1.0 and 2.0 microgram/kg i.v. ALEX or HEX on GH, PRL, ACTH, cortisol and aldosterone levels and those of 20mg p.o. ( approximately 300 microgram/kg) on GH levels. RESULTS: Basal GH, PRL, ACTH, cortisol and aldosterone levels in all testing sessions were similar. ALEX and HEX (1.0 and 2.0 microgram/kg i.v.) induced the same dose-dependent increase of GH and PRL levels. Both ALEX and HEX induced a dose-dependent increase of ACTH and cortisol levels. The ACTH and cortisol responses to the highest ALEX dose were significantly higher than those after HEX. Aldosterone levels significantly increased after both i.v. ALEX doses, but not after HEX. The GH response to 20mg p.o. ALEX was higher, though not significantly, than that to the same HEX dose. CONCLUSION: ALEX, a new GHS, shows the same GH-releasing activity as HEX. On the other hand, ALEX seems endowed with an ACTH-releasing activity more marked than that of HEX; this evidence could explain the significant increase of aldosterone levels after its i.v. administration.

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Chromosomal damage and mitotic index in Bosnian and Herzegovinian mountain horse lymphocytes following low dosage X-ray irradiation in vitro

Veterinarski glasnik ◽

10.2298/vetgl0906341r ◽

2009 ◽

Vol 63 (5-6) ◽

pp. 341-352

Author(s):

Dunja Rukavina ◽

Danica Hasanbasic ◽

Edin Suljkanovic ◽

Amela Katica

Keyword(s):

Mitotic Index ◽

Analysis Of Variance ◽

The Other ◽

Chromosomal Damage ◽

X Ray ◽

Dose Dependent Decrease ◽

Dose Dependent Increase ◽

Dose Dependent ◽

Low Dosage

In this study we investigated the influence of low dosage X - ray irradiation on the incidence of chromosomal damage and changes in mitotic index (MI) in cultured peripheral lymphocytes of the Bosnian and Herzegovinian mountain horse following in vitro irradiation. X-ray irradiation induced a dose-dependent decrease in MI but only the dose of 0.5 Gy induced a significant decrease (p<0.05) in comparison with the control and other dose groups. The analysis of chromosomal damage revealed a clear dose-dependent increase in the incidence of chromosomal damage per metaphase. Significant differences (p<0.05) were detected by analysis of variance and the LSD test confirmed significant differences between cells that received 0.2 Gy and 0.5 Gy when compared to the control cells and cells that received 0.1 Gy. However, Scheeffes' test assigned significance only to the differences established between the cells that received 0.5 Gy and the other groups of lymphocytes. .

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Effect of Prostaglandins A1, A2, B1, E2 and F2α on Forearm Arterial Bed and Superficial Hand Veins in Man

Clinical Science ◽

10.1042/cs0440367 ◽

1973 ◽

Vol 44 (4) ◽

pp. 367-376 ◽

Cited By ~ 35

Author(s):

B. F. Robinson ◽

J. G. Collier ◽

S. M. M. Karim ◽

K. Somers

Keyword(s):

Blood Flow ◽

Blood Vessels ◽

Brachial Artery ◽

Peripheral Blood ◽

Forearm Blood Flow ◽

The Other ◽

Dose Dependent Increase ◽

Dose Dependent

1. The effects of local infusions of prostaglandins (PG) A1, A2, B1, E2 and F2α have been studied in the forearm arterial bed and superficial hand veins of man. 2. Prostaglandins A1, A2, B1, E2 and F2α all gave rise to a dose-dependent increase in forearm blood flow when infused into the brachial artery. At dosages just below the dilator range, PGF2α caused a transient fall in forearm flow, but this was not seen with any of the other prostaglandins. 3. Prostaglandins A1, A2 and E2 had no effect when infused locally into relaxed veins but caused dose-dependent dilatation when given into veins preconstricted with either noradrenaline or 5-hydroxytryptamine. Prostaglandins B1 and F2α caused dose-dependent constriction when given into relaxed veins and had no dilator effect when infused into preconstricted veins. 4. The results suggest that there are at least two types of receptors mediating responses to prostaglandins in peripheral blood vessels.

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Increased Expression of u-PA and u-PAR on Monocytes by LDL and Lp(a) Lipoproteins – Consequences for Plasmin Generation and Monocyte Adhesion

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614531 ◽

1999 ◽

Vol 81 (04) ◽

pp. 594-560 ◽

Cited By ~ 10

Author(s):

Florence Ganné ◽

Marc Vasse ◽

Jean-Louis Beaudeu ◽

Jacqueline Peynet ◽

Arnaud François ◽

...

Keyword(s):

Fold Increase ◽

Surface Expression ◽

Cell Surface Expression ◽

Atheromatous Plaque ◽

Monocyte Adhesion ◽

Blood Monocytes ◽

Proteolytic Cascade ◽

Ecm Degradation ◽

Dose Dependent Increase ◽

Dose Dependent

SummaryMonocyte-derived foam cells figure prominently in rupture-prone regions of atherosclerotic plaque. As urokinase/urokinase-receptor (u-PA/u-PAR) is the trigger of a proteolytic cascade responsible for ECM degradation, we have examined the effect of atherogenic lipoproteins on monocyte surface expression of u-PAR and u-PA. Peripheral blood monocytes, isolated from 10 healthy volunteers, were incubated with 10 to 200 µg/ml of native or oxidised (ox-) atherogenous lipoproteins for 18 h and cell surface expression of u-PA and u-PAR was analysed by flow cytometry. Both LDL and Lp(a) induced a dose-dependent increase in u-PA (1.6-fold increase with 200 μg/ml of ox-LDL) and u-PAR [1.7-fold increase with 200 μg/ml of ox-Lp(a)]. There is a great variability of the response among the donors, some of them remaining non-responders (absence of increase of u-PA or u-PAR) even at 200 μg/ml of lipoproteins. In positive responders, enhanced u-PA/u-PAR is associated with a significant increase of plasmin generation (1.9-fold increase with 200 μg/ml of ox-LDL), as determined by an amidolytic assay. Furthermore, monocyte adhesion to vitronectin and fibrinogen was significantly enhanced by the lipoproteins [respectively 2-fold and 1.7-fold increase with 200 μg/ml of ox-Lp(a)], due to the increase of u-PAR and ICAM-1, which are receptors for vitronectin and fibrinogen. These data suggest that atherogenous lipoproteins could contribute to the development of atheromatous plaque by increasing monocyte adhesion and trigger plaque weakening by inducing ECM degradation.

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LH-RH TEST IN 100 PATIENTS WITH OVARIAN INSUFFICIENCY

Acta Endocrinologica ◽

10.1530/acta.0.0750428 ◽

1974 ◽

Vol 75 (3) ◽

pp. 428-434 ◽

Cited By ~ 5

Author(s):

P.-J. Czygan ◽

M. Breckwoldt ◽

F. Lehmann ◽

R. Langefeld ◽

G. Bettendorf

Keyword(s):

Intravenous Injection ◽

Functional State ◽

Clear Correlation ◽

Normal Range ◽

Ovarian Insufficiency ◽

Lh Rh ◽

Dose Dependent Increase ◽

Dose Dependent

ABSTRACT The effect of synthetic LH-RH was studied in 100 patients with various types of ovarian insufficiency by following up the FSH- and LH-levels in plasma. LH-RH was administered in doses of 12.5, 25 and 100 μg as a rapid intravenous injection. The patients were classified according to the endocrine state of the pituitary as evidenced by the urinary gonadotrophin levels. A clear correlation between the functional state of the pituitary and its responsiveness to exogenous LH-RH was demonstrated. Most of the patients with undetectable low urinary gonadotrophin levels failed to respond. The majority of patients with gonadotrophin excretion in the normal range and those with elevated levels reacted with a dose dependent increase in circulating LH. The amount of liberated FSH however was related to the injected dose only in patients with high gonadotrophic excretion. The present study indicates that synthetic LH-RH provides a useful tool in the evaluation of the pitutiary function particularly in patients with low and with undetectable gonadotrophin excretion. The data presented in this paper also demonstrate that the functional state of the pituitary is clearly reflected by the urinary gonadotrophin levels.

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