Isolation and Identification of Endophytic Fungi in Kernels of Coix lachrymal-jobi L. Cultivars

Phylloplane biodiversity and endophytic fungi is considered one of the rich origins of novel biological activity compounds and high-level structural variation on the leaf surface. Plant leaves surface is a diverse terrestrial ecosystem, including filamentous fungi. This study aims to study the isolation and enumeration of Sapindus mukorossi phylloplane and endophytic fungal diversity. The Sapindus mukorossi isolated 14 fungal species from 9 genera of phylloplane and endophytic fungi. Cladosporium herbarum, Penicillium expansum, Fusarium oxysporum, Fusarium sp., Alternaria alternate, Collectotrichum orbiculare, Torulla herbarium, Epicoccum nigrum and Candida sp. as a phylloplane fungi. Aspergillus niger, A. flavus, Epicoccum nigrum. Penicillum digitatum, Penicillum sp. were identified as endophytic fungi.

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Identification of Endophytic Fungi from Rhizome Galanga (Alpinia galanga L.) against Candida albicans

Metamorfosa: Journal of Biological Sciences ◽

10.24843/metamorfosa.2018.v05.i02.p10 ◽

2018 ◽

Vol 5 (2) ◽

pp. 205

Author(s):

Ni Made Raningsih ◽

Putu Wahyu Sri Juniantari Sandy

Keyword(s):

Candida Albicans ◽

Endophytic Fungi ◽

Trichoderma Harzianum ◽

Food Microbiology ◽

Microbiology Laboratory ◽

Isolation And Identification ◽

Alpinia Galanga

The main objectives of this research were to isolat and identify endophytic fungi antagonists of Candida albicnas from galanga rhizomes. Samples of galanga rhizome were collected from Gitgit plantation in Gitgit village, Buleleng regency. Isolation and identification were conducted in Food Microbiology Laboratory, Faculty of Agriculture, Udayana University. There were three isolates identified with inhibitory persentage againts Candida albicans i.e. Trichoderma viridae (26.07±3.76%), Trichoderma harzianum (45.11±5.84%) and Fusarium oxyforum (20.11±5.61%).

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Peer Review #3 of "Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran (v0.3)"

10.7287/peerj.8309v0.3/reviews/3 ◽

2020 ◽

Author(s):

T Islam

Keyword(s):

Peer Review ◽

Plant Species ◽

Endophytic Fungi ◽

Isolation And Identification ◽

Asteraceae Family

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Fungal Endophytes of Vitis vinifera—Plant Growth Promotion Factors

Agriculture ◽

10.3390/agriculture11121250 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1250

Author(s):

Markéta Kulišová ◽

Maria Vrublevskaya ◽

Petra Lovecká ◽

Blanka Vrchotová ◽

Milena Stránská ◽

...

Keyword(s):

Vitis Vinifera ◽

Endophytic Fungi ◽

Growth Promotion ◽

Fungal Endophytes ◽

Plant Tissues ◽

Antifungal Compounds ◽

Microscopic Fungi ◽

Isolation And Identification ◽

Wide Range ◽

Promising Source

Endophytes are microorganisms that live asymptomatically inside plant tissues. They are beneficial to their host in many aspects, especially as a defense against foreign phytopathogens through the production of a variety of metabolites. These substances can serve as sources of new natural products for medicinal, agricultural, and industrial purposes. This article is focused on endophytic fungi from Vitis vinifera. The purpose of the research was their isolation and identification during the Vitis vinifera growing season. Subsequently, the isolates were tested for the production of biotechnologically interesting metabolites (siderophores, antioxidants, and antifungal compounds). In total, 24 endophytic fungi were isolated, the most represented genus was Cladosporium sp. The results of the test for antioxidant and antifungal properties, as well as siderophore production, have shown that the population of Vitis vinifera endophytic microscopic fungi could serve as a promising source of metabolites with a wide range of applications.

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Isolation and identification of endophytic fungi from mandarin orange (Citrus reticulata L.)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/951/1/012052 ◽

2022 ◽

Vol 951 (1) ◽

pp. 012052

Author(s):

H Oktarina ◽

D R Adithia ◽

T Chamzurni

Keyword(s):

Abiotic Stress ◽

Secondary Metabolites ◽

Endophytic Fungi ◽

Morphological Characteristic ◽

Plant Disease ◽

Potato Dextrose Agar ◽

Citrus Reticulata ◽

Biotic And Abiotic Stress ◽

Isolation And Identification ◽

Mandarin Orange

Abstract Endophytic fungi are recognised for producing secondary metabolites that have an effect on the host, such as promoting growth and enhancing plant resilience to biotic and abiotic stress. The objective of this study was to explore endophytic fungi from Citrus reticulata. The endophytic fungi were isolated from both healthy and infected roots, stems, and twigs of C. reticulata. The materials were sterilised and inoculated on potato dextrose agar (PDA) media. The fungi grown were transferred onto fresh PDA plates and identified based on their morphological characteristic, including colonial features, hyphae type, and reproduction structure. Five endophytic fungi were identified as Colletotrichum sp. AJSH2-1, Cylindrocladium sp. BJSH1-2, Rhizopus sp. RJSH1-1, Mucor sp. RJSH1-2, and Aspergillus sp. BJSH1-1. Further study is required to understand the ability of the identified genera in protecting the host from plant disease as well as promoting growth.

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Isolation and Identification of Endophytic Fungi of Urena lobata L. Cultivated in Amazon

Journal of Agricultural Science ◽

10.5539/jas.v10n10p316 ◽

2018 ◽

Vol 10 (10) ◽

pp. 316

Author(s):

Carla Coelho Ferreira ◽

Henrique dos Santos Pereira ◽

Albejamere Pereira de Castro ◽

Pedro de Queiroz Costa Neto ◽

Kelven Wladie dos Santos Almeida Coelho

Keyword(s):

Seed Production ◽

Endophytic Fungi ◽

Cropping Systems ◽

Isolation And Identification ◽

Crop Fields ◽

Plant Organ ◽

Commercial Scale ◽

Amazonas State ◽

Local Farmers ◽

Spontaneous Occurrence

Fiber of Urena lobata L. (Caesar weed or Malva) has been produced from plants cultivated in floodplain areas of Brazilian Amazonas state since 1970s. Plants must be harvested before flooding and because of such a short cultivation season plants never reach fructification. Thus, local farmers depend on acquiring seeds collected in areas of spontaneous occurrence in drylands to sow their crop fields each year. This scenario might change, as this species is gaining socioeconomic important and there is interest in the development of cropping systems for seed production on a commercial scale. The objective of this study was to investigate the occurrence of endophytic fungi in U. lobata, evaluating the influence of the plant organ (leaf and seed) and farming site (two experimental fields of seed production: Manaus and Anori municipalities), once endophytic interaction can be beneficial or become harmful to the plants. The frequency and degree of colonization, Shannon-Weaver and Simpson diversity and Pielou uniformity indexes and the dominance component curve were calculated. The occurring genus were: Phomopsis, Colletotrichum, Fusarium, Pestalotiopsis, Curvularia and Cochliobolus. The greatest diversity and richness of fungi was found in Manaus site with high colonization present in the leaves. The genus Curvularia and Fusarium presented preference for seeds while Colletotrichum was more frequent in leaves. The similarity was greater between plant organs than between the farming sites, which indicates that the coevolution of these fungi and its host has resulted in a balanced mutual or antagonistic interaction (Crane & Acuna, 1945; Souza, 2012).

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