Impact of nitrogen metabolism-associated culture pH changes on regulation of Fusarium trichothecene biosynthesis: revision of roles of polyamine agmatine and transcription factor AreA

2020 ◽  
Vol 66 (6) ◽  
pp. 1179-1190 ◽  
Author(s):  
Yuichi Nakajima ◽  
Manami Akasaka ◽  
Takuya Shiobara ◽  
Yoshiyuki Kitou ◽  
Kazuyuki Maeda ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Ph Changes ◽  
Culture Ph

scholarly journals Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

2020 ◽  
Vol 21 (3) ◽  
pp. 774
Author(s):  
Xuemei Xiao ◽  
Jian Lv ◽  
Jianming Xie ◽  
Zhi Feng ◽  
Ning Ma ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Expression Patterns ◽  
Calvin Cycle ◽  
Biomass Accumulation ◽  
Tca Cycle ◽  
Mapk Signaling ◽  
Carbon And Nitrogen ◽  
Related Transcription Factor ◽  
Carbon And Nitrogen Metabolism

Autotoxicity of root exudates is one of the main reasons for consecutive monoculture problem (CMP) in cucumber under greenhouse cultivation. Rootstock grafting may improve the tolerance of cucumber plants to autotoxic stress. To verify the enhanced tolerance to autotoxic stress and illuminate relevant molecular mechanism, a transcriptomic comparative analysis was performed between rootstock grafted (RG) and non-grafted (NG) cucumber plants by a simulation of exogenous cinnamic acid (CA). The present study confirmed that relatively stable plant growth, biomass accumulation, chlorophyll content, and photosynthesis was observed in RG than NG under CA stress. We identified 3647 and 2691 differentially expressed genes (DEGs) in NG and RG cucumber plants when compared to respective control, and gene expression patterns of RNA-seq was confirmed by qRT-PCR. Functional annotations revealed that DEGs response to CA stress were enriched in pathways of plant hormone signal transduction, MAPK signaling pathway, phenylalanine metabolism, and plant-pathogen interaction. Interestingly, the significantly enriched pathway of photosynthesis-related, carbon and nitrogen metabolism only identified in NG, and most of DEGs were down-regulated. However, most of photosynthesis, Calvin cycle, glycolysis, TCA cycle, and nitrogen metabolism-related DEGs exhibited not or slightly down-regulated in RG. In addition, several stress-related transcription factor families of AP2/ERF, bHLH, bZIP, MYB. and NAC were uniquely triggered in the grafted cucumbers. Overall, the results of this study suggest that rootstock grafting improve the tolerance of cucumber plants to autotoxic stress by mediating down-regulation of photosynthesis, carbon, and nitrogen metabolism-related DEGs and activating the function of stress-related transcription factor. The transcriptome dataset provides an extensive sequence resource for further studies of autotoxic mechanism at molecular level.

The extended regulon of the Escherichia coli LgoR transcription factor includes genes for sugar acid and nitrogen metabolism

2016 ◽  
Vol 33 ◽  
pp. S177
Author(s):  
Maria Tutukina ◽  
Uliana Shvyreva ◽  
Inna Suvorova ◽  
Diana Gagarinskaya ◽  
Olga Ozoline ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Nitrogen Metabolism

scholarly journals Ipa1 Improves Rice Drought Tolerance at Seedling Stage Mainly Through Activating Abscisic Acid Pathway

2021 ◽  
Author(s):  
Menghao Zhu ◽  
Yonggang He ◽  
Mingqiang Zhu ◽  
Ayaz Ahmad ◽  
Shuang Xu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Nitrogen Metabolism ◽  
Crop Production ◽  
Plant Architecture ◽  
Biotic Resistance ◽  
Rice Seedling ◽  
Seedling Stage ◽  
Luciferase Assay ◽  
Aba Biosynthesis

Abstract Drought is a major abiotic stress to crop production. IPA1 (IDEAL PLANT ARCHITECTURE 1)/OsSPL14 encodes a transcription factor and has been reported to function in both rice ideal plant architecture and biotic resistance. Here, with a pair of IPA1/ipa1-NILs (Near Iso-genic Lines), we found that ipa1 could significantly improve rice drought tolerance at seedling stage. The ipa1 plants had a better-developed root system and smaller leaf stomatal aperture. Analysis of carbon-nitrogen metabolism-associated enzyme activity, gene expression, and metabolic profile indicated that ipa1 could tip the carbon-nitrogen metabolism balance towards an increased carbon metabolism pattern. In both the control and PEG-treated conditions, ABA content in the ipa1 seedlings was significantly higher than that in the IPA1 seedlings. Expression of the ABA biosynthesis genes was detected to be up-regulated, whereas the expression of ABA catabolism genes was down-regulated in the ipa1 seedlings. In addition, based on yeast one-hybrid assay and dual-luciferase assay, IPA1 was found to directly activate the promoter activity of OsHOX12, a transcription factor promoting ABA biosynthesis, and OsNAC52, a positive regulator of the ABA pathway. The expression of OsHOX12 and OsNAC52 was significantly up-regulated in the ipa1 plants. Combined with the previous studies, our results suggested that ipa1 could improve rice seedling drought tolerance mainly through activating the ABA pathway and that regulation of the ipa1-mediated ABA pawthway will be an important strategy for improving drought resistance of rice.

scholarly journals A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism

PLoS Genetics ◽  
2017 ◽  
Vol 13 (5) ◽  
pp. e1006737 ◽  
Author(s):  
Yi Xiong ◽  
Vincent W. Wu ◽  
Andrea Lubbe ◽  
Lina Qin ◽  
Siwen Deng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Starch Degradation ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism

The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae

2020 ◽  
Vol 226 (5) ◽  
pp. 1461-1479 ◽  
Author(s):  
Chen Tang ◽  
Tianyu Li ◽  
Steven J. Klosterman ◽  
Chengming Tian ◽  
Yonglin Wang
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Verticillium Dahliae ◽  
Bzip Transcription Factor

The overexpression of the pine transcription factor PpDof5 inArabidopsisleads to increased lignin content and affects carbon and nitrogen metabolism

2015 ◽  
Vol 155 (4) ◽  
pp. 369-383 ◽  
Author(s):  
Marina Rueda-López ◽  
Rafael A. Cañas ◽  
Javier Canales ◽  
Francisco M. Cánovas ◽  
Concepción Ávila
Keyword(s):  
Transcription Factor ◽  
Nitrogen Metabolism ◽  
Lignin Content ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression
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