Abscisic acid controls embryo growth potential and endosperm cap weakening during coffee (Coffea arabica cv. Rubi) seed germination

Planta ◽  
2004 ◽  
Vol 220 (2) ◽  
pp. 251-261 ◽  
Author(s):  
E. A. Amaral da Silva ◽  
Peter E. Toorop ◽  
Adriaan C. van Aelst ◽  
Henk W. M. Hilhorst
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Coffea Arabica ◽  
Growth Potential

The expansin EXP1 gene in the elongation zone is induced during soybean embryonic axis germination and differentially expressed in response to ABA and PEG treatments

2020 ◽  
pp. 1-9
Author(s):  
Nidia H. Montechiarini ◽  
Luciana Delgado ◽  
Eligio N. Morandi ◽  
Néstor J. Carrillo ◽  
Carlos O. Gosparini
Keyword(s):  
Cell Wall ◽  
Seed Germination ◽  
Water Availability ◽  
Expression Profiles ◽  
Soybean Seed ◽  
Embryonic Axis ◽  
Growth Potential ◽  
Elongation Zone ◽  
Degenerate Primers ◽  
Embryonic Axes

Abstract During soybean seed germination, the expansive growth potential of the embryonic axes is driven by water uptake while cell wall loosening occurs in cells from the elongation zone (EZ). Expansins are regarded as primary promoters of cell wall remodelling in all plant expansion processes, with the expression profiles of the soybean expansins supporting their cell or tissue specificity. Therefore, we used embryonic axes isolated from whole seed and focused on the EZ to study seed germination. Using a suite of degenerate primers, we amplified an abundantly expressed expansin gene at the EZ during soybean embryonic axis germination, which was identified as EXP1 by in silico analyses. Expression studies showed that EXP1 was induced under germination conditions in distilled water and down-regulated by abscisic acid (ABA), which inhibits soybean germination by physiologically restraining expansion. Moreover, we also identified a time window of ABA responsiveness within the first 6 h of incubation in water, after which ABA lost control of both EXP1 expression and embryonic axis germination, thus confirming the early role of EXP1 in the EZ during this process. By contrast, EXP1 levels in the EZ increased even when germination was impaired by osmotically limiting the water availability required to develop the embryonic axes’ growth potential. We propose that these higher EXP1 levels are involved in the fast germination of soybean embryonic axes as soon as water availability is re-established. Taken together, our results show strong EXP1 expression in the EZ and postulate EXP1 as a target candidate for soybean seed germination control.

Abscisic acid and reactive oxygen species were involved in slightly acidic electrolyzed water-promoted seed germination in watermelon

2022 ◽  
Vol 291 ◽  
pp. 110581
Author(s):  
Xue Wu ◽  
Cuinan Wu ◽  
Zhonghua Bian ◽  
Zhangying Ye ◽  
Lili Meng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Abscisic Acid ◽  
Seed Germination ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Electrolyzed Water

16-O-Demethyl Fusicoccin J and Its 3-Epimer fromFusicoccum amygdali, and Their Seed Germination-stimulating Activity in the Presence of Abscisic Acid

1999 ◽  
Vol 63 (5) ◽  
pp. 951-954 ◽  
Author(s):  
Takeshi SASSA ◽  
Chang-Shan ZHANG ◽  
Naoto TAJIMA ◽  
Nobuo KATO
Keyword(s):  
Abscisic Acid ◽  
Seed Germination

scholarly journals Effects of hormonal priming on seed germination of pigeon pea under cadmium stress

2015 ◽  
Vol 87 (3) ◽  
pp. 1847-1852 ◽  
Author(s):  
LARISSA C. SNEIDERIS ◽  
MARINA A. GAVASSI ◽  
MARCELO L. CAMPOS ◽  
VICTOR D'AMICO-DAMIÃO ◽  
ROGÉRIO F. CARVALHO
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Efficient Method ◽  
Physiological Responses ◽  
Cadmium Stress ◽  
Pigeon Pea ◽  
Metal Stress ◽  
Hormonal Priming

In this work we investigated whether priming with auxin, cytokinin, gibberellin, abscisic acid and ethylene, alters the physiological responses of seeds of pigeon pea germinated under water and cadmium stress. Seeds treated with water or non-treated seeds were used as control. Although compared to non-treated seeds we found that the hormone treatments improve the germination of pigeon pea under cadmium stress, however, these treatments did not differ from water. However, we also observed a trend of tolerance to the effects of cadmium in the presence of ethylene, suggesting that the use of this hormone may be an efficient method to overcome seed germination under metal stress.

scholarly journals Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 inhibit WRKY75 function in abscisic acid-mediated leaf senescence and seed germination

2021 ◽  
Author(s):  
Haiyan Zhang ◽  
Liping Zhang ◽  
Yunrui Ji ◽  
Yifen Jing ◽  
Lanxin Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Leaf Senescence ◽  
Chromatin Immunoprecipitation ◽  
Sigma Factor ◽  
Dependent Manner ◽  
Negative Regulators ◽  
Factor Binding ◽  
Physiological Processes ◽  
Increased Sensitivity

Abstract The plant-specific VQ gene family participates in diverse physiological processes but little information is available on their role in leaf senescence. Here, we show that the VQ motif-containing proteins, Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 are negative regulators of abscisic acid (ABA)-mediated leaf senescence. Loss of SIB1 and SIB2 function resulted in increased sensitivity of ABA-induced leaf senescence. In contrast, overexpression of SIB1 significantly delayed this process. Moreover, biochemical studies revealed that SIBs interact with WRKY75 transcription factor. Loss of WRKY75 function decreased sensitivity to ABA-induced leaf senescence, while overexpression of WRKY75 significantly accelerated this process. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoters of GOLDEN 2-LIKE1(GLK1) and GLK2, to repress their expression. SIBs repress the transcriptional function of WRKY75 and negatively regulate ABA-induced leaf senescence in a WRKY75-dependent manner. In contrast, WRKY75 positively modulates ABA-mediated leaf senescence in a GLK-dependent manner. In addition, SIBs inhibit WRKY75 function in ABA-mediated seed germination. These results demonstrate that SIBs can form a complex with WRKY75 to regulate ABA-mediated leaf senescence and seed germination.

scholarly journals Gibberellic acid, paclobutrazol, fluoridone, abscisic acid affecting flowering and fruit set of coffee

1969 ◽  
Vol 89 (3-4) ◽  
pp. 159-168
Author(s):  
Carlos A. Flores ◽  
Winston De la Torre ◽  
Miguel Monroig ◽  
Wigmar González
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Coffea Arabica ◽  
Fruit Set ◽  
Late Development ◽  
Flower Bud ◽  
Initial Reduction ◽  
Coffea Arabica L

Applications of gibberellic acid (GA), paclobutrazol and gibberellic acid (Paclo/GA), fluoridone and gibberellic acid (FL/GA), and abscisic acid (ABA) were made to synchronize flowering in coffee trees (Coffea arabica L.). Overall growth of trees and branches was not affected by the treatments. Production of new leaves was not affected by the treatments. However, the Paclo/GA treatment tended to increase the production of secondary branches. Flower bud production was affected by the ABA treatment during the first three weeks, showing an initial reduction in number and a late development of buds. Plants receiving the GA treatment produced more flowers during the first week of evaluation as well as a higher number and greater weight of mature fruits during the first week of harvesting. 

Determination of radiosensitivity of Coffea arabica var. 'Venecia' seeds to gamma-ray irradiation.

2021 ◽  
pp. 320-325
Author(s):  
Reina Céspedes ◽  
Noel Arrieta ◽  
Miguel Barquero ◽  
Ana Abdelnour ◽  
Nielen Stephan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Coffea Arabica ◽  
Gamma Ray ◽  
Raw Materials ◽  
New Technology ◽  
Mutation Breeding ◽  
Mutation Induction ◽  
Rust Disease ◽  
Sources Of Resistance ◽  
Breeding Programmes

Abstract Coffee is one of the most commercially available raw materials, being the tropical product with the highest market value in the world. In Costa Rica it is the third most important product for agricultural exports and provides the main income for many families in the country. However, coffee is under threat due to coffee leaf rust disease (CLR). Mutation breeding in coffee is a promising approach to develop new varieties resistant to CLR. As a new technology for coffee, basic tests related to mutation induction need to be done. The plant material used was Coffea arabica var. 'Venecia' seeds, with a moisture content of 27.3%. The applied irradiation doses were 0, 80, 100, 120, 140, 160 and 180 Gy. For each treatment, three replicates of 200 g were used, with a seed number range of 765-808 units per replicate. The irradiated seeds were planted on the same day. Eighty days after treatment the number of seedlings was quantified, the hypocotyl height and radicle length were measured and the opening of cotyledons was determined for each dose. The effects of the radiation doses on seed germination frequency were recorded. At the dose of 80 Gy, germination was reduced over the control by 9.65%, at 100 Gy by 34.06%, at 120 Gy by 52.76%, at 140 Gy by 60.24%, at 160 Gy by 65.56% and at 180 Gy by 75.40%. Seedling growth was affected and a delay in opening of the cotyledons was observed at higher doses. This radiosensitivity test, based on seed germination as compared with unirradiated control, revealed that the LD50 for the variety tested is in the range 100-120 Gy experimentally, and according to the regression is 125 ± 30 Gy. This dose will be used for further bulk experiments and is of great importance, because the LD50 is considered as the range where the appearance of useful mutations in breeding programmes is favoured. The establishment of these parameters is a necessary advance to continue with measurements of genetic and phenotypical parameters to implement mutation breeding in coffee looking for new sources of resistance against CLR.

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