The floral volatile, methyl benzoate, from snapdragon (Antirrhinum majus) triggers phytotoxic effects in Arabidopsis thaliana

Planta ◽  
2007 ◽  
Vol 226 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Jun-ichiro Horiuchi ◽  
Dayakar V. Badri ◽  
Bruce A. Kimball ◽  
Florence Negre ◽  
Natalia Dudareva ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Methyl Benzoate ◽  
Antirrhinum Majus ◽  
Phytotoxic Effects ◽  
Floral Volatile

scholarly journals Molecular Cloning and Functional Characterization of CpMYC2 and CpBHLH13 Transcription Factors from Wintersweet (Chimonanthus praecox L.)

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 785
Author(s):  
Muhammad Zeshan Aslam ◽  
Xiang Lin ◽  
Xiang Li ◽  
Nan Yang ◽  
Longqing Chen
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Transgenic Plants ◽  
Jasmonic Acid ◽  
Volatile Compounds ◽  
Floral Scent ◽  
Functional Characterization ◽  
High Expression ◽  
Floral Volatile ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox L.) is an ornamental and economically significant shrub known for its unique flowering characteristics, especially the emission of abundant floral volatile organic compounds. Thus, an understanding of the molecular mechanism of the production of these compounds is necessary to create new breeds with high volatile production. In this study, two bHLH transcription factors (CpMYC2 and CpbHLH13) of Wintersweet H29 were functionally characterized to illustrate their possible role in the production of volatile compounds. The qRT-PCR results showed that the expression of CpMYC2 and CpbHLH13 increased from the flower budding to full bloom stage, indicating that these two genes may play an essential role in blooming and aroma production in wintersweet. Gas chromatography-mass spectroscopy (GC-MS) analysis revealed that the overexpression of CpMYC2 in arabidopsis (Arabidopsis thaliana) AtMYC2-2 mutant (Salk_083483) and tobacco (Nicotiana tabaccum) genotype Petit Havana SR1 significantly increased floral volatile monoterpene, especially linalool, while the overexpression of CpbHLH13 in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and tobacco genotype SR1 increased floral sesquiterpene β-caryophyllene production in both types of transgenic plants respectively. High expression of terpene synthase (TPS) genes in transgenic A. thaliana along with high expression of CpMYC2 and CpbHLH13 in transgenic plants was also observed. The application of a combination of methyl jasmonic acid (MeJA) and gibberellic acid (GA3) showed an increment in linalool production in CpMYC2-overexpressing arabidopsis plants, and the high transcript level of TPS genes also suggested the involvement of CpMYC2 in the jasmonic acid (JA) signaling pathway. These results indicate that both the CpMYC2 and CpbHLH13 transcription factors of wintersweet are possibly involved in the positive regulation and biosynthesis of monoterpene (linalool) and sesquiterpene (β-caryophyllene) in transgenic plants. This study also indicates the potential application of wintersweet as a valuable genomic material for the genetic modification of floral scent in other flowering plants that produce less volatile compounds.

scholarly journals Phytotoxic effects of trichothecenes on the growth and morphology of Arabidopsis thaliana

2007 ◽  
Vol 58 (7) ◽  
pp. 1617-1626 ◽  
Author(s):  
D. Masuda ◽  
M. Ishida ◽  
K. Yamaguchi ◽  
I. Yamaguchi ◽  
M. Kimura ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phytotoxic Effects

The regulation of flowering in Arabidopsis thaliana: meristems, morphogenesis, and mutants

1995 ◽  
Vol 73 (7) ◽  
pp. 959-981 ◽  
Author(s):  
George W. Haughn ◽  
Elizabeth A. Schultz ◽  
Jose M. Martinez-Zapater
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Floral Development ◽  
Regulatory Proteins ◽  
Antirrhinum Majus ◽  
Complete Understanding ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial ◽  
Floral Evocation ◽  
Key Questions

In the last decade, the study of mutants defective in floral development has contributed significantly to our understanding of floral evocation and morphogenesis. Genes in Arabidopsis thaliana and Antirrhinum majus that play key roles in (i) the transition from the vegetative to reproductive phase, (ii) the activation of floral development in specific shoots, and (iii) the unique arrangement of floral organs have been identified genetically and in many cases cloned. Many of the genes appear to encode transcription factors that act to select specific developmental programs of division and differentiation for groups of primordial cells. Other genes may be involved in detecting environmental conditions and transducing the signal to the developing meristems. Key questions remaining include how the regulatory proteins are produced in specific temporal and spatial patterns, interact with each other and initiate specific morphological programs. Although current research on floral morphogenesis has been limited to only a few species there is growing evidence that the basic processes are common to all flowering plants.Thus the information and tools currently being generated should be useful for studying a wide variety of flowering species. It seems reasonable to predict that within the next decade, we should have a fairly complete understanding of the basic mechanisms underlying floral morphogenesis and its evolution among the angiosperms. Key words: Arabidopsis thaliana, floral morphogenesis, molecular genetics.

Genetic complementation of a floral homeotic mutation, apetala3, with an Arabidopsis thaliana gene homologous to DEFICIENS of Antirrhinum majus

1994 ◽  
Vol 26 (1) ◽  
pp. 465-472 ◽  
Author(s):  
Hiroyuki Okamoto ◽  
Azusa Yano ◽  
Hideaki Shiraishi ◽  
Kiyotaka Okada ◽  
Yoshiro Shimura
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Complementation ◽  
Antirrhinum Majus ◽  
Homeotic Mutation ◽  
Floral Homeotic

scholarly journals Bumblebee electric charge stimulates floral volatile emissions in Petunia integrifolia but not in Antirrhinum majus

2021 ◽  
Vol 108 (5) ◽  
Author(s):  
Clara Montgomery ◽  
Jozsef Vuts ◽  
Christine M. Woodcock ◽  
David M. Withall ◽  
Michael A. Birkett ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Electric Charge ◽  
Flowering Plants ◽  
Antirrhinum Majus ◽  
Volatile Emissions ◽  
Flying Insects ◽  
Floral Volatile ◽  
Electrically Charged ◽  
Sensory Process ◽  
Petunia Integrifolia

AbstractThe timing of volatile organic compound (VOC) emission by flowering plants often coincides with pollinator foraging activity. Volatile emission is often considered to be paced by environmental variables, such as light intensity, and/or by circadian rhythmicity. The question arises as to what extent pollinators themselves provide information about their presence, in keeping with their long co-evolution with flowering plants. Bumblebees are electrically charged and provide electrical stimulation when visiting plants, as measured via the depolarisation of electric potential in the stem of flowers. Here we test the hypothesis that the electric charge of foraging bumblebees increases the floral volatile emissions of bee pollinated plants. We investigate the change in VOC emissions of two bee-pollinated plants (Petunia integrifolia and Antirrhinum majus) exposed to the electric charge typical of foraging bumblebees. P. integrifolia slightly increases its emissions of a behaviorally and physiologically active compound in response to visits by foraging bumblebees, presenting on average 121 pC of electric charge. We show that for P. integrifolia, strong electrical stimulation (600–700 pC) promotes increased volatile emissions, but this is not found when using weaker electrical charges more representative of flying pollinators (100 pC). Floral volatile emissions of A. majus were not affected by either strong (600–700 pC) or weak electric charges (100 pC). This study opens a new area of research whereby the electrical charge of flying insects may provide information to plants on the presence and phenology of their pollinators. As a form of electroreception, this sensory process would bear adaptive value, enabling plants to better ensure that their attractive chemical messages are released when a potential recipient is present.

Heterologous myb genes distinct from GL1 enhance trichome production when overexpressed in Nicotiana tabacum

Development ◽  
1999 ◽  
Vol 126 (4) ◽  
pp. 671-682 ◽  
Author(s):  
T. Payne ◽  
J. Clement ◽  
D. Arnold ◽  
A. Lloyd
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Nicotiana Tabacum ◽  
Cell Shape ◽  
Cellular Differentiation ◽  
Antirrhinum Majus ◽  
Tobacco Plants ◽  
Developmental Abnormalities ◽  
Myb Gene ◽  
Myb Genes

Myb-class transcription factors implicated in cell shape regulation were overexpressed in Arabidopsis thaliana and Nicotiana tabacum in an attempt to assess the extent to which cellular differentiation programs might be shared between these distantly related plants. GLABROUS 1, a myb gene required for trichome development in Arabidopsis, did not alter the trichome phenotype of the tobacco plants in which it was overexpressed. MIXTA, which in Antirrhinum majus is reported to regulate certain aspects of floral papillae development, did not complement the glabrous 1 mutant of Arabidopsis. However, 35S:MIXTA transformants of N. tabacum displayed various developmental abnormalities, most strikingly production of supernumerary trichomes on cotyledons, leaves and stems. In addition, floral papillae were converted to multicellular trichomes. CotMYBA, a myb gene which is expressed in Gossypium hirsutum ovules and has some homology to MIXTA, was also overexpressed in the two species. A similar but distinct syndrome of abnormalities, including the production of cotyledonary trichomes, was observed in 35S:CotMYBA tobacco transformants. However, CotMYBA did not alter trichome production in Arabidopsis. These results suggest that the trichomes of Arabidopsis and Nicotiana are merely analogous structures, and that the myb genes regulating their differentiation are specific and separate.

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