Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis

2010 ◽  
Vol 108 (1) ◽  
pp. 211-217 ◽  
Author(s):  
Nadda Kiatsopit ◽  
Paiboon Sithithaworn ◽  
Jiraporn Sithithaworn ◽  
Thidarut Boonmars ◽  
Smarn Tesana ◽  
...  
Keyword(s):  
Species Complex ◽  
Genetic Relationships ◽  
Lao Pdr ◽  
Opisthorchis Viverrini ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Specific Analysis

scholarly journals Analysis of the population genetics of Opisthorchis viverrini sensu lato in the Nam Ngum River wetland, Lao PDR, by multilocus enzyme electrophoresis

2014 ◽  
Vol 113 (8) ◽  
pp. 2973-2981 ◽  
Author(s):  
Nadda Kiatsopit ◽  
Paiboon Sithithaworn ◽  
Weerachai Saijuntha ◽  
Opal Pitaksakulrat ◽  
Trevor N. Petney ◽  
...  
Keyword(s):  
Population Genetics ◽  
Lao Pdr ◽  
Opisthorchis Viverrini ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

scholarly journals Genetic relationships amongNeisseria gonorrhoeaeserovars analysed by multilocus enzyme electrophoresis

1992 ◽  
Vol 108 (1) ◽  
pp. 31-38 ◽  
Author(s):  
C. L. Poh ◽  
J. C. Ocampo ◽  
G. K. Loh
Keyword(s):  
Genetic Diversity ◽  
Neisseria Gonorrhoeae ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Electrophoretic Analysis ◽  
Enzyme Electrophoresis ◽  
Enzyme Locus ◽  
Multilocus Enzyme Electrophoresis ◽  
Enzyme Loci ◽  
The Mean

Multilocus enzyme electrophoretic analysis was employed to assess the genetic relatedness ofNeisseria gonorrhoeae. Based on the diversity of electromorphs at 9 enzyme loci, 16 electrophoretic types (ETs) were estabilished amongst the 65 isolates. The average number of alleles per enzyme locus was 1·7 and the mean genetic diversity per locus was 0·212. The majority of isolates belonged to either ET1 (32·3%) or ET2 (16·9%). No specific correlation of ETs was seen with serovars as the major types, ETs 1 and 2, were found distributed amongst the various serovars. Major serovars such as Bacjk (IB-1/2) and Bajk (IB-3/6) were each represented by 6 or 8 ETs respectively. Analysis of the genetic relationships of ETs to each other showed some clustering of subgroups that were more closely related than others.

scholarly journals Genetic diversity among toxigenic and nontoxigenicVibrio choleraeO1 isolated from the Western Hemisphere

1991 ◽  
Vol 107 (1) ◽  
pp. 225-233 ◽  
Author(s):  
F. Chen ◽  
G. M. Evins ◽  
W. L. Cook ◽  
R. Almeida ◽  
N. Hargrett-Bean ◽  
...  
Keyword(s):  
Gulf Coast ◽  
Genetic Relationships ◽  
Western Hemisphere ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
The Us ◽  
Single Clone ◽  
Diverse Origin ◽  
Toxigenic Strains ◽  
Surrounding Areas

SUMMARYMultilocus enzyme electrophoresis was used to examine genetic relationships among and between toxigenic and non-toxigenic isolates ofVibrio choleraeO1 obtained from patients and the environment in the US Gulf Coast and surrounding areas. A total of 23 toxigenic and 23 non-toxigenic strains were examined. All the toxigenic and 7 of the non-toxigenic strains had the same alleles at 16 enzyme loci, whereas the balance of the nontoxigenic strains had 9 distinct combinations of alleles. This study suggests that all of the toxigenic strains belong to a single clone, and that while some of the non-toxigenic isolates were related, most were of diverse origin.

Genetic differentiation among populations ofBrachytrupes portentosus(Lichtenstein 1796) (Orthoptera: Gryllidae) in Thailand and the Lao PDR: the Mekong River as a biogeographic barrier

2011 ◽  
Vol 101 (6) ◽  
pp. 687-696 ◽  
Author(s):  
C. Tantrawatpan ◽  
W. Saijuntha ◽  
W. Pilab ◽  
K. Sakdakham ◽  
P. Pasorn ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Genetic Relationships ◽  
Lao Pdr ◽  
Natural Populations ◽  
Mekong River ◽  
Enzyme Electrophoresis ◽  
Freshwater Species ◽  
Intermediate Hosts ◽  
Mammal Species ◽  
Terrestrial Invertebrates

AbstractThe Mekong River is known to act as a boundary between a number of terrestrial and freshwater species, including various parasites and their intermediate hosts as well as endangered mammal species. Little information is available, however, on the genetic differentiation between terrestrial invertebrates to the east and the west of this wide river. The genetic diversity among eight natural populations ofBrachytrupes portentosus(Lichtenstein, 1796) (Orthoptera: Gryllidae) collected from Thailand and the Lao People's Democratic Republic (PDR) were analyzed by multilocus enzyme electrophoresis. The allelic profiles of 20 enzymes encoding 23 loci were analyzed. An average of 41% fixed differences was detected between the populations from Thailand and Lao PDR, which are separated by the Mekong River. The percent fixed differences ranged between 4% and 26% within the populations from Thailand and between 4% and 22% within the populations from Lao PDR. A phenogram shows that the eight populations fell into two major clusters based on the Thai and Lao sampling sites. The genetic distance between the samples within Thailand and within Lao PDR was related to the distances between sampling areas. The genetic variability between populations of this cricket indicates that genetic relationships are influenced by a natural barrier as well as by the geographical distance between these allopatric populations.

scholarly journals Genetic relationships amongEscherichia coliisolates causing urinary tract infections in humans and animals

1989 ◽  
Vol 102 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Thomas S. Whittam ◽  
M. L. Wolfe ◽  
R. A. Wilson
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Genetic Relationships ◽  
Enzyme Electrophoresis ◽  
Bootstrap Analysis ◽  
Multilocus Enzyme Electrophoresis ◽  
Total Genetic Diversity ◽  
Major Cluster ◽  
The Common ◽  
Tract Infections

SUMMARYGenetic variation in isolates ofEscherichia coliobtained mostly from urinary tract infections in humans and domesticated animals (dogs and cats) was assessed for 16 enzymes using multilocus enzyme electrophoresis to characterize chromosomal genotypes. A total of 148 isolates comprised 63 distinct electrophoretic types (ETs) and about half of the isolates belonged to one of 9 common ETs. A bootstrap analysis of genetic distance between ETs revealed three significant groups of strains. Variation in allele frequencies among groups accounted for 40% of the total genetic diversity. The majority of the common ETs fell into a major cluster of closely related strains. The recovery of multiple isolates of the same electrophoretic types and serotypes from unassociated hosts suggests that these bacteria represent uropathogenic clones that are widely disseminated in humans and animals

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