scholarly journals Use of multilocus enzyme electrophoresis to examine genetic relationships amongst isolates of Mycobacterium intracellulare and related species

Microbiology ◽  
1997 ◽  
Vol 143 (4) ◽  
pp. 1461-1469 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. J. Dawson ◽  
D. J. Hampson
Keyword(s):  
Related Species ◽  
Genetic Relationships ◽  
Enzyme Electrophoresis ◽  
Mycobacterium Intracellulare ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

scholarly journals Genetic relationships amongNeisseria gonorrhoeaeserovars analysed by multilocus enzyme electrophoresis

1992 ◽  
Vol 108 (1) ◽  
pp. 31-38 ◽  
Author(s):  
C. L. Poh ◽  
J. C. Ocampo ◽  
G. K. Loh
Keyword(s):  
Genetic Diversity ◽  
Neisseria Gonorrhoeae ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Electrophoretic Analysis ◽  
Enzyme Electrophoresis ◽  
Enzyme Locus ◽  
Multilocus Enzyme Electrophoresis ◽  
Enzyme Loci ◽  
The Mean

Multilocus enzyme electrophoretic analysis was employed to assess the genetic relatedness ofNeisseria gonorrhoeae. Based on the diversity of electromorphs at 9 enzyme loci, 16 electrophoretic types (ETs) were estabilished amongst the 65 isolates. The average number of alleles per enzyme locus was 1·7 and the mean genetic diversity per locus was 0·212. The majority of isolates belonged to either ET1 (32·3%) or ET2 (16·9%). No specific correlation of ETs was seen with serovars as the major types, ETs 1 and 2, were found distributed amongst the various serovars. Major serovars such as Bacjk (IB-1/2) and Bajk (IB-3/6) were each represented by 6 or 8 ETs respectively. Analysis of the genetic relationships of ETs to each other showed some clustering of subgroups that were more closely related than others.

scholarly journals Genetic diversity among toxigenic and nontoxigenicVibrio choleraeO1 isolated from the Western Hemisphere

1991 ◽  
Vol 107 (1) ◽  
pp. 225-233 ◽  
Author(s):  
F. Chen ◽  
G. M. Evins ◽  
W. L. Cook ◽  
R. Almeida ◽  
N. Hargrett-Bean ◽  
...  
Keyword(s):  
Gulf Coast ◽  
Genetic Relationships ◽  
Western Hemisphere ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
The Us ◽  
Single Clone ◽  
Diverse Origin ◽  
Toxigenic Strains ◽  
Surrounding Areas

SUMMARYMultilocus enzyme electrophoresis was used to examine genetic relationships among and between toxigenic and non-toxigenic isolates ofVibrio choleraeO1 obtained from patients and the environment in the US Gulf Coast and surrounding areas. A total of 23 toxigenic and 23 non-toxigenic strains were examined. All the toxigenic and 7 of the non-toxigenic strains had the same alleles at 16 enzyme loci, whereas the balance of the nontoxigenic strains had 9 distinct combinations of alleles. This study suggests that all of the toxigenic strains belong to a single clone, and that while some of the non-toxigenic isolates were related, most were of diverse origin.

scholarly journals Genetic relationships amongEscherichia coliisolates causing urinary tract infections in humans and animals

1989 ◽  
Vol 102 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Thomas S. Whittam ◽  
M. L. Wolfe ◽  
R. A. Wilson
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Genetic Relationships ◽  
Enzyme Electrophoresis ◽  
Bootstrap Analysis ◽  
Multilocus Enzyme Electrophoresis ◽  
Total Genetic Diversity ◽  
Major Cluster ◽  
The Common ◽  
Tract Infections

SUMMARYGenetic variation in isolates ofEscherichia coliobtained mostly from urinary tract infections in humans and domesticated animals (dogs and cats) was assessed for 16 enzymes using multilocus enzyme electrophoresis to characterize chromosomal genotypes. A total of 148 isolates comprised 63 distinct electrophoretic types (ETs) and about half of the isolates belonged to one of 9 common ETs. A bootstrap analysis of genetic distance between ETs revealed three significant groups of strains. Variation in allele frequencies among groups accounted for 40% of the total genetic diversity. The majority of the common ETs fell into a major cluster of closely related strains. The recovery of multiple isolates of the same electrophoretic types and serotypes from unassociated hosts suggests that these bacteria represent uropathogenic clones that are widely disseminated in humans and animals

scholarly journals Clonal Population Structure of Pseudomonas stutzeri, a Species with Exceptional Genetic Diversity

2001 ◽  
Vol 183 (2) ◽  
pp. 736-744 ◽  
Author(s):  
Núria Rius ◽  
M. Carme Fusté ◽  
Caterina Guasp ◽  
Jorge Lalucat ◽  
José G. Lorén
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Relationships ◽  
Bacterial Species ◽  
Pseudomonas Stutzeri ◽  
Null Alleles ◽  
Enzyme Electrophoresis ◽  
Single Strain ◽  
Multilocus Enzyme Electrophoresis ◽  
Clonal Population Structure

ABSTRACT Genetic diversity and genetic relationships among 42Pseudomonas stutzeri strains belonging to several genomovars and isolated from different sources were investigated in an examination of 20 metabolic enzymes by multilocus enzyme electrophoresis analysis. Forty-two distinct allele profiles were identified, indicating that all multilocus genotypes were represented by a single strain. All 20 loci were exceptionally polymorphic, with an average of 15.9 alleles per locus. To the best of our knowledge, thisP. stutzeri sample exhibited the highest mean genetic diversity (H = 0.876) found to date in all bacterial species studied by multilocus enzyme electrophoresis. A high frequency of occurrence of null alleles was identified. The index of association (IA ) for the P. stutzeri strains analyzed was 1.10. The IA values were always significantly different from zero for all subgroups studied, including clinical and environmental isolates and strains classified as genomovar 1. These results suggest that the population structure of P. stutzeri is strongly clonal, indicating that there is no significant level of assortative recombination that might destroy linkage disequilibrium.

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