Morphological and molecular identification of Hysterothylacium larvae (Nematoda: Raphidascarididae) in marine fish from Tunisian Mediterranean coasts

2020 ◽  
Vol 119 (10) ◽  
pp. 3285-3296
Author(s):  
Manel Khammassi ◽  
Sihem Bahri ◽  
Gokmen Zafer Pekmezci
Keyword(s):  
Marine Fish ◽  
Molecular Identification

scholarly journals Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France

2019 ◽  
Vol 10 ◽  
Author(s):  
Gabriela Certad ◽  
Jérôme Follet ◽  
Nausicaa Gantois ◽  
Ourida Hammouma-Ghelboun ◽  
Karine Guyot ◽  
...  
Keyword(s):  
Risk Factors ◽  
Marine Fish ◽  
Molecular Identification ◽  
Cryptosporidium Infection

scholarly journals Correction: Regev, Y., et al. Molecular Identification and Characterization of Vibrio Species and Mycobacterium Species in Wild and Cultured Marine Fish from the Eastern Mediterranean Sea. Microorganisms 2020, 8, 863

2020 ◽  
Vol 8 (8) ◽  
pp. 1153
Author(s):  
Yael Regev ◽  
Nadav Davidovich ◽  
Ran Berzak ◽  
Stanley C. K. Lau ◽  
Aviad P. Scheinin ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Marine Fish ◽  
Molecular Identification ◽  
Eastern Mediterranean ◽  
Vibrio Species ◽  
Mycobacterium Species ◽  
Eastern Mediterranean Sea ◽  
Identification And Characterization

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scholarly journals Environmental DNA (eDNA) metabarcoding: Diversity study around the Pondok Dadap fish landing station, Malang, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Sapto Andriyono ◽  
MD. JOBAIDUL ALAM ◽  
HYUN-WOO KIM
Keyword(s):  
Marine Fish ◽  
Molecular Identification ◽  
Environmental Dna ◽  
Fish Diversity ◽  
Morphological Identification ◽  
Fish Samples ◽  
Identification Of Species ◽  
Seawater Samples ◽  
Next Generation Sequencing Ngs ◽  
Diversity Study

Abstract. Andriyono S, Jobaidul Alam Md, Kim HW. 2019. Environmental DNA (eDNA) metabarcoding: Diversity study around the Pondok Dadap fish landing station, Malang, Indonesia. Biodiversitas 20: 3772-3781. Molecular identification of species is now fast growing and currently widely applied method in the diversity estimation of aquatic biota; even though morphological identification is still carried out. The molecular approach is beneficial complementing on regular surveys, e.g. use of nets, traps, fishing rods, and even with poisons. In this study, the eDNA metabarcoding was applied to water samples around the Pondok Dadap fish landing station, Indonesia to determine the diversity of fish around the waters and also to identify marine fish landed in this area. Molecular identification was carried out on fish samples obtained from the fish market improved GenBank database on COI and ITS. While, seawater samples were carried out by using the next-generation sequencing (NGS) platform to obtain the eDNA metabarcoding data for the first time. Molecular identification obtained 34 species (68 sequences of COI and ITS regions) belonging to 28 genera, 18 families, 4 orders, while the eDNA metabarcoding approach identified 53 marine fish species by using the MiFish pipeline representing 38 genera, 27 families, and 7 orders. From the present study, we can able to estimated fish diversity by eDNA metabarcoding, and this finding will be helpful for baseline data preparation for future effective management of resources in this area.

scholarly journals Molecular Identification of Adenocephalus pacificus (Cestoda) from Three Human Cases in Lima Province, Peru

2020 ◽  
Vol 58 (4) ◽  
pp. 457-460
Author(s):  
Aarón Mondragón-Martínez ◽  
Rosa Martínez-Rojas ◽  
Enrique Garcia-Candela ◽  
Abraham Delgado-Escalante ◽  
Manuel Tantaleán-Vidaurre ◽  
...  
Keyword(s):  
Cytochrome C ◽  
South America ◽  
Cytochrome C Oxidase ◽  
Marine Fish ◽  
Molecular Identification ◽  
Causative Agent ◽  
Pacific Coast ◽  
The Other ◽  
Partial Sequence ◽  
The Pacific

The Pacific tapeworm Adenocephalus pacifcus (syn. Diphyllobothrium pacificum) is a causative agent of diphyllobothriosis occurred in Pacific coast of South America, mainly in Peru. Source of infections are traditional meal from raw or undercooked marine fish such as “cebiche”. We confirmed 3 new cases, one including scolex and the other two headless. A strobila 46 cm long without scolex was discharged from an 8-year-old boy before treatment. Specimens were confirmed morphologically by presence of tegumental protuberances on proglottids and small sized eggs. Partial sequence of cytochrome c oxidase subunit 1 gene was congruent with A. pacificus sequences.

scholarly journals Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia

2014 ◽  
Vol 52 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Hilal Anshary ◽  
Sriwulan ◽  
Mark A. Freeman ◽  
Kazuo Ogawa
Keyword(s):  
Marine Fish ◽  
Molecular Identification

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

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