The Jawa and Bali Island Marine Fish Molecular Identification to Improve 12S rRNA-tRNA Valin-16S rRNA Partial Region Sequences on the GenBank Database

2020 ◽  
Vol 36 (2) ◽  
pp. 343-356
Author(s):  
Sapto Andriyono ◽  
Md. Jobaidul Alam ◽  
Hyun-Woo Kim
Keyword(s):  
16S Rrna ◽  
Marine Fish ◽  
Molecular Identification ◽  
12S Rrna ◽  
Partial Region

scholarly journals Sequence Analysis of Mitochondrial DNAs of 12S rRNA, 16S rRNA, and Cytochrome Oxidase Subunit 1(COI) Regions in Slow Lorises (Genus Nycticebus) May Contribute to Improved Identification of Confiscated Specimens

ISRN Zoology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Hiroko Somura ◽  
Hiroshi Hori ◽  
Yoshinobu Manome
Keyword(s):  
16S Rrna ◽  
Cytochrome Oxidase ◽  
Dna Analysis ◽  
12S Rrna ◽  
Cytochrome Oxidase Subunit ◽  
Slow Loris ◽  
Oxidase Subunit ◽  
Mitochondrial Dnas ◽  
Cytochrome Oxidase Subunit 1 ◽  
Rrna 16S

The slow loris (Nycticebus) is a prosimian that is popular among exotic pet lovers. In Japan, many slow lorises have been imported illegally. Prosimians that have been confiscated in raids are protected in Japanese zoos, and the number of such animals has increased. In most cases, the country of origin remains unknown and even the species can be difficult to identify from the animal’s physical appearance alone. We have attempted to resolve this problem by using DNA analysis. DNA samples of five species, consisting of the Pygmy slow loris (Nycticebus pygmaeus), Bengal slow loris (Nycticebus bengalensis), Sunda slow loris (Nycticebus coucang), Javan slow loris (Nycticebus javanicus), and Bornean slow loris (Nycticebus menagensis), were extracted, amplified, and the nucleotide sequences of mitochondrial 12S rRNA, 16S rRNA, and the cytochrome oxidase subunit 1(COI) regions were compared. Differences of nucleic acid sequences of representative individuals were demonstrated.

scholarly journals Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France

2019 ◽  
Vol 10 ◽  
Author(s):  
Gabriela Certad ◽  
Jérôme Follet ◽  
Nausicaa Gantois ◽  
Ourida Hammouma-Ghelboun ◽  
Karine Guyot ◽  
...  
Keyword(s):  
Risk Factors ◽  
Marine Fish ◽  
Molecular Identification ◽  
Cryptosporidium Infection

Relationships of scincid lizards (Mabuya spp.) from the islands of the Gulf of Guinea based on mtDNA sequence data

2005 ◽  
Vol 26 (4) ◽  
pp. 467-473 ◽  
Author(s):  
José Jesus ◽  
D. James Harris ◽  
António Brehm
Keyword(s):  
16S Rrna ◽  
Species Complex ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
12S Rrna ◽  
Gulf Of Guinea ◽  
Recent Proposal ◽  
Genetic Lineages ◽  
Sao Tome And Principe ◽  
Mtdna Sequence

AbstractRelationships of Mabuya lizards from the islands of the Gulf of Guinea where estimated using partial 12S rRNA, 16S rRNA and cytochrome b mitochondrial gene sequences. Mabuya maculilabris from São Tomé and Principe are discreet monophyletic units, highly divergent from each other and from mainland populations, indicating M. maculilabris may be a species complex. Mabuya affinis from Principe is similarly distinct from mainland populations of this species. The relationships of Mabuya ozorii from Annobon are unclear, but the three species in the Gulf of Guinea islands are not closely related, indicating multiple independent colonization events. The recent proposal to partition Mabuya into four genera is premature, since at least five distinct genetic lineages can be identified.

scholarly journals Morphological and molecular identification of hairtail (Trichiurus spp.) caught in Pangandaran Waters

2020 ◽  
Vol 147 ◽  
pp. 02021
Author(s):  
Tia Aprianti Lestari ◽  
Murwantoko Murwantoko ◽  
Eko Setyobudi
Keyword(s):  
Principal Component Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Identification ◽  
Principal Component ◽  
Component Analysis ◽  
Rrna Gene ◽  
Morphometric Variation ◽  
Dorsal Fin ◽  
Fish Samples

This study aimed to identify the species of hairtail caught in Pengandaran waters based on morphological, meristic character and molecular approach. In total 135 fish samples were collected from Pangandaran Waters, during March-April 2017. Each sample was identified, measured on 22 morphometric and 4 meristic characters, then analyzed using Principal Component Analysis (PCA). Molecular identification was conducted by sequenced of 16S rRNA gene. The result of the research showed that hairtail characterized by III spines and 125-140 soft rays of dorsal fin (D.III, 125-140), the anal fin situated below 38th to 41th of dorsal-fin soft ray, I spine and 10 soft rays of pectoral fin (P.I.10), and I spine and 91 to 112 spinules of anal fin (A.I.91-112). Based on the morphological identification, the hairtail was belonged to Trichiurus lepturus. Principal Component Analysis showing the morphometric variation was presented in the caudal peduncle length. Molecular analysis of mitochondrial DNA of the partial 16S rRNA gene confirmed the hairtail as T. lepturus with similarity 98-99% based on previously published data. Phylogenetic analysis showed that T. lepturus from Pangandaran were closely similar to related species caught from the Southern Coast of Yogyakarta Special Territory (Indian Ocean) and Hainan China (Pacific Ocean).

Phylogeny of Dorippoidea (Crustacea : Decapoda : Brachyura) inferred from three mitochondrial genes

2009 ◽  
Vol 23 (3) ◽  
pp. 223 ◽  
Author(s):  
Y. W. Sin ◽  
Joelle C. Y. Lai ◽  
Peter K. L. Ng ◽  
K. H. Chu
Keyword(s):  
Molecular Markers ◽  
Cytochrome C ◽  
16S Rrna ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
Mitochondrial Genes ◽  
12S Rrna ◽  
Generic Relationships ◽  
I Gene

The phylogenetic relationships between 10 of 13 genera of crabs from the superfamily Dorippoidea were investigated using mitochondrial 16S rRNA, 12S rRNA and cytochrome c oxidase subunit I gene sequences. The resultant phylogenetic trees based on the three molecular markers support the division of Dorippidae and Ethusidae as monophyletic families within the Dorippoidea. The inferred inter-generic relationships within Dorippidae concur with groupings based on the overall morphology of the carapace and structures of the male first pleopods.

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