A block of endocytosis of the yeast cell wall integrity sensors Wsc1 and Wsc2 results in reduced fitness in vivo

2010 ◽  
Vol 284 (3) ◽  
pp. 217-229 ◽  
Author(s):  
Sabrina Wilk ◽  
Janina Wittland ◽  
Andreas Thywissen ◽  
Hans-Peter Schmitz ◽  
Jürgen J. Heinisch
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall

scholarly journals Transcription factors of M-phase cyclin CLB2 in the yeast cell wall integrity checkpoint

2009 ◽  
Vol 84 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Mizuho Sekiya ◽  
Satoru Nogami ◽  
Yoshikazu Ohya
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Yeast Cell ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
M Phase

scholarly journals Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling

2016 ◽  
Vol 18 (9) ◽  
pp. 1251-1267 ◽  
Author(s):  
Christian Kock ◽  
Henning Arlt ◽  
Christian Ungermann ◽  
Jürgen J. Heinisch
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Yeast Cell ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
Membrane Microdomains ◽  
Plasma Membrane Microdomains

Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2

FEBS Letters ◽  
2013 ◽  
Vol 587 (22) ◽  
pp. 3675-3680 ◽  
Author(s):  
José M. Rodríguez-Peña ◽  
Sonia Díez-Muñiz ◽  
Clara Bermejo ◽  
César Nombela ◽  
Javier Arroyo
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Mapk Pathway ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall

scholarly journals Up against the Wall: Is Yeast Cell Wall Integrity Ensured by Mechanosensing in Plasma Membrane Microdomains?

2014 ◽  
Vol 81 (3) ◽  
pp. 806-811 ◽  
Author(s):  
Christian Kock ◽  
Yves F. Dufrêne ◽  
Jürgen J. Heinisch
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Yeast Cell ◽  
Antifungal Drugs ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
Membrane Microdomains ◽  
Wall Structure ◽  
Fungal Cell ◽  
Membrane Spanning

ABSTRACTYeast cell wall integrity (CWI) signaling serves as a model of the regulation of fungal cell wall synthesis and provides the basis for the development of antifungal drugs. A set of five membrane-spanning sensors (Wsc1 to Wsc3, Mid2, and Mtl1) detect cell surface stress and commence the signaling pathway upon perturbations of either the cell wall structure or the plasma membrane. We here summarize the latest advances in the structure/function relationship primarily of the Wsc1 sensor and critically review the evidence that it acts as a mechanosensor. The relevance and physiological significance of the information obtained for the function of the other CWI sensors, as well as expected future developments, are discussed.

scholarly journals An Analog-sensitive Version of the Protein Kinase Slt2 Allows Identification of Novel Targets of the Yeast Cell Wall Integrity Pathway

2016 ◽  
Vol 291 (11) ◽  
pp. 5461-5472 ◽  
Author(s):  
Esmeralda Alonso-Rodríguez ◽  
Pablo Fernández-Piñar ◽  
Almudena Sacristán-Reviriego ◽  
María Molina ◽  
Humberto Martín
Keyword(s):  
Cell Wall ◽  
Protein Kinase ◽  
Yeast Cell ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
Cell Wall Integrity Pathway ◽  
Novel Targets

scholarly journals Comprehensive Evaluation of the Efficiency of Yeast Cell Wall Extract to Adsorb Ochratoxin A and Mitigate Accumulation of the Toxin in Broiler Chickens

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 37 ◽  
Author(s):  
Suvi Vartiainen ◽  
Alexandros Yiannikouris ◽  
Juha Apajalahti ◽  
Colm A. Moran
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Ochratoxin A ◽  
Broiler Chickens ◽  
Animal Feed ◽  
Animal Health ◽  
Yeast Cell Wall ◽  
In Vivo Model

Ochratoxin A (OTA) is a common mycotoxin contaminant in animal feed. When absorbed from the gastrointestinal tract, OTA has a propensity for pathological effects on animal health and deposition in animal tissues. In this study, the potential of yeast cell wall extracts (YCWE) to adsorb OTA was evaluated using an in vitro method in which consecutive animal digestion events were simulated. Low pH markedly increased OTA binding to YCWE, which was reversed with a pH increased to 6.5. Overall, in vitro analysis revealed that 30% of OTA was adsorbed to YCWE. Additional computational molecular modelling revealed that change in pH alters the OTA charge and modulates the interaction with the YCWE β-d-glucans. The effectiveness of YCWE was tested in a 14-day broiler chicken trial. Birds were subjected to five dietary treatments; with and without OTA, and OTA combined with YCWE at three dosages. At the end of the trial, liver OTA deposition was evaluated. Data showed a decrease of up to 30% in OTA deposits in the liver of broilers fed both OTA and YCWE. In the case of OTA, a tight correlation between the mitigation efficacy of YCWE between in vitro and in vivo model could be observed.

scholarly journals Regulation of LRG1 expression by RNA‐binding protein Puf5 in the budding yeast cell wall integrity pathway

2018 ◽  
Vol 23 (12) ◽  
pp. 988-997 ◽  
Author(s):  
Nguyen Thi Minh Viet ◽  
Duong Long Duy ◽  
Kazuhiro Saito ◽  
Kaoru Irie ◽  
Yasuyuki Suda ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Rna Binding ◽  
Budding Yeast ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Cell Wall Integrity ◽  
Yeast Cell Wall ◽  
Cell Wall Integrity Pathway

In vitro zearalenone adsorption by a mixture of organic and inorganic adsorbents: application of the Box Behnken approach

2015 ◽  
Vol 8 (3) ◽  
pp. 291-299 ◽  
Author(s):  
J.G. Bordini ◽  
D. Borsato ◽  
A.S. Oliveira ◽  
M.A. Ono ◽  
T.H. Zaninelli ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Activated Charcoal ◽  
High Efficiency ◽  
Statistical Design ◽  
Yeast Cell Wall ◽  
Quadratic Model ◽  
Wide Range

Zearalenone (ZEA) adsorption by a mixture of organic (yeast cell wall) and inorganic (activated charcoal) adsorbents was evaluated by an incomplete Box Behnken (33) statistical design with a quintuplicate at the central point. The variables analysed were different ratios of adsorbents (yeast cell wall and activated charcoal) at 100:0, 87.5:12.5 and 75:25, pH (3.0, 4.5 and 6.0) and ZEA concentrations (300, 750 and 1,200 ng/ml). The adsorbent mixture at 75:25 showed higher efficiency for ZEA adsorption (≯96.1%) than the 87.5:12.5 ratio (81.3 to 93.7%) and with the pure yeast cell wall (78.1 to 55.7%). The significant variables were the ratio of adsorbent mixture and ZEA concentration. The effect of pH was not significant (P=0.05), indicating that the binding between ZEA and the adsorbent would be stable at different pH (3.0, 4.5 and 6.0). The quadratic model obtained by the Box Behnken (33) design can be used for predictive purposes, because it showed a non-significant deviation (P=49.54%) and a good correlation coefficient (R2=0.98), suggesting that the ZEA adsorption would be maximum (100%) when the adsorbent mixture is set at 75:25 and the ZEA concentration at 300 ng/ml. Although the predictive model showed that an increase in adsorption efficiency could occur in a smaller ZEA concentration (300 ng/ml), the mixture at the 75:25 ratio presented high efficiency (≯98%) in adsorption when high ZEA concentrations were used (1,200 ng/ml), indicating that these mixtures would be able to adsorb a wide range of ZEA concentrations. Therefore, this mixture of yeast cell wall and activated charcoal adsorbents at 75:25 might be a candidate for further in vivo testing.

Efficacy of b-glucans and manna oligosaccharides (Yeast Cell Wall) and hydrated sodium calcium aluminosilicate (HSCAS) in preventing aflatoxicosis in bovine calves

2017 ◽  
Author(s):  
Omer Naseer ◽  
Jawaria Khan ◽  
Muhammad Khan ◽  
Muhammad Omer ◽  
Muhammad Avais ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Hematological Parameters ◽  
Negative Control ◽  
Yeast Cell Wall ◽  
Feed Consumption ◽  
Sodium Calcium ◽  
Calcium Aluminosilicate

The objective of this study was to determine the response of bovine calves against aflatoxin B1 (AFB1) in terms of feed consumption, hematological and serum biochemical parameters and to compare the efficacy of two different mycotoxin adsorbents, in vitro and in vivo. 36 bovine calves were divided into 4 groups. Group A was fed AFB1 added feed with the addition of â-glucans and Mannan oligosaccharides (Yeast Cell Wall), group B was fed AFB1 with hydrated sodium calcium aluminosilicate (HSCAS) and group C was fed AFB1 contaminated feed without addition of mycotoxin binders while group D was kept as negative control. AFB1 was given by gelatinized capsules at a dose rate of 1.0mg/ kg/ animal/ day. Results revealed average daily feed intake (ADFI) of AFB1 treated bovine calves significantly reduced (P less than 0.05) and all hematological parameters i.e; TEC, HGB, TLC, lymphocytes, neutrophils and monocytes, MCHC, HCT and MCH decreased significantly (P less than 0.05). Moreover, serum levels of AST, ALT, Creatinine and BUN were significantly increased (P less than 0.05) in response to AFB1. When compared between groups, YCW significantly (P less than 0.05) improved the feed consumption of bovine calves while HSCAS significantly reduced (P less than 0.05) the AFB1 induced deleterious alterations in hematology and serum biochemistry.

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