Unveiling diversity of stem cells in dental pulp and apical papilla using mouse genetic models: a literature review

Each odontoblast is tightly linked to other odontoblasts. They form a line of defense and are capable of withstanding external stimuli, particularly the inflammation caused by caries. Thus, we investigated exosomes derived from odontoblasts as an intercellular mechanism by which inflamed odontoblasts are protected from apoptosis. CD63, an exosome marker, was expressed at high levels in caries-affected regions of the dental pulp. We conducted an ex vivo experiment by applying different concentrations of lipopolysaccharide (LPS) to the odontoblast-like cells (mineralization was induced in stem cells derived from the apical papilla). Odontoblast-like cells treated with a high concentration of LPS (20 µg/mL LPS, severely affected) exhibited an accelerated release of exosomes, which attenuated the LPS-induced cell apoptosis of odontoblast-like cells treated with a low concentration of LPS (1 µg/mL LPS, mildly affected). Next, we blocked exosome uptake with chlorpromazine, and the rescue effect vanished. Based on our findings, severely inflamed odontoblasts attenuate the apoptosis of mildly inflamed neighboring cells through an exosome-mediated intercellular signaling pathway.

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Effect of the Soluble Factors Released by Dental Apical Papilla-Derived Stem Cells on the Osteo/Odontogenic, Angiogenic, and Neurogenic Differentiation of Dental Pulp Cells

Stem Cells and Development ◽

10.1089/scd.2019.0262 ◽

2020 ◽

Vol 29 (12) ◽

pp. 795-805 ◽

Cited By ~ 3

Author(s):

Shi Yu ◽

Yuming Zhao ◽

Teng Jiaozi Fang ◽

Lihong Ge

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Soluble Factors ◽

Neurogenic Differentiation ◽

Pulp Cells ◽

Apical Papilla

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Dental Pulp Stem Cells, a New Era in Regenerative Medicine: A Literature Review

Open Journal of Stomatology ◽

10.4236/ojst.2016.66020 ◽

2016 ◽

Vol 06 (06) ◽

pp. 155-163

Author(s):

Manar Aljamie ◽

Lujain Alessa ◽

Rawan Noah ◽

Lubna Elsayed

Keyword(s):

Stem Cells ◽

Regenerative Medicine ◽

Literature Review ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

New Era

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Dental stem cells and their application in Dentistry: a literature review

Revista Brasileira de Odontologia ◽

10.18363/rbo.v73n4.p.331 ◽

2016 ◽

Vol 73 (4) ◽

pp. 331 ◽

Cited By ~ 2

Author(s):

Paula Nascimento Almeida ◽

Karin Soares Cunha

Keyword(s):

Stem Cells ◽

Literature Review ◽

Bone Repair ◽

Permanent Teeth ◽

Deciduous Teeth ◽

Dental Stem Cells ◽

Dental Origin ◽

Apical Papilla ◽

Regenerative Therapies ◽

Interesting Alternative

Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. Material and Methods: for this, we selected scientific articles published between 2000 and 2016 through the databases PUBMED and LILACS. Results: there are five main sources of stem cells of dental origin: stem cells from dental pulp of permanent teeth and deciduous teeth, apical papilla, periodontal ligament and dental follicle. These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry.

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Dental-Derived Mesenchymal Stem Cells: State of the Art

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.654559 ◽

2021 ◽

Vol 9 ◽

Author(s):

Bo Li ◽

Takehito Ouchi ◽

Yubin Cao ◽

Zhihe Zhao ◽

Yi Men

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Tissue Regeneration ◽

Dental Pulp ◽

State Of The Art ◽

Neural Cells ◽

Immune Microenvironment ◽

Injury Repair ◽

Apical Papilla ◽

Mammalian Teeth

Mesenchymal stem cells (MSCs) could be identified in mammalian teeth. Currently, dental-derived MSCs (DMSCs) has become a collective term for all the MSCs isolated from dental pulp, periodontal ligament, dental follicle, apical papilla, and even gingiva. These DMSCs possess similar multipotent potential as bone marrow-derived MSCs, including differentiation into cells that have the characteristics of odontoblasts, cementoblasts, osteoblasts, chondrocytes, myocytes, epithelial cells, neural cells, hepatocytes, and adipocytes. Besides, DMSCs also have powerful immunomodulatory functions, which enable them to orchestrate the surrounding immune microenvironment. These properties enable DMSCs to have a promising approach in injury repair, tissue regeneration, and treatment of various diseases. This review outlines the most recent advances in DMSCs’ functions and applications and enlightens how these advances are paving the path for DMSC-based therapies.

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