scholarly journals Epithelial cell turnover in relation to ongoing damage of the gastric mucosa in patients with early gastric cancer: increase of cell proliferation in paramalignant lesions

2005 ◽  
Vol 40 (4) ◽  
pp. 337-344 ◽  
Author(s):  
Akiko Shiotani ◽  
Hiroyasu Iishi ◽  
Shingo Ishiguro ◽  
Masaharu Tatsuta ◽  
Yukinori Nakae ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Epithelial Cell ◽  
Gastric Mucosa ◽  
Early Gastric Cancer ◽  
Cell Turnover

scholarly journals ATP4B suppresses cell proliferation and migration via mitochondrial/p53/NF-κB pathway in gastric cancer

2020 ◽  
Author(s):  
Yuanming Pan ◽  
Yuqi He ◽  
Shuye Lin ◽  
Min Zhu ◽  
Yangjie Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Gastric Mucosa ◽  
Tumor Model ◽  
Suppressor Gene ◽  
Mitochondrial Pathway ◽  
Loss Of Function ◽  
Normal Tissues ◽  
And Migration ◽  
Induced Apoptosis

Abstract Background: Hydrogen/Potassium ATPase β (ATP4B) is a key protein in gastric mucosa barrier acting an essential role in gastric acid secretion. However, the exact role and precise mechanism of ATP4B in gastric cancer (GC) remain obscure. This study aimed to investigate the clinical significance of ATP4B in GC and its biological role in tumor progression.Methods: We evaluated ATP4B expression in GC cell lines and patient specimens via qPCR and Immunofluorescence. The correlations between ATP4B expression level and clinicopathologic parameters, as well as the relevance of ATP4B expression with overall survival were assessed. The functional roles of ATP4B in GC were verified by gain- and loss-of-function cell models and xenograft tumor model. The possible downstream effects of ATP4B were analyzed by iTRAQ‑based quantitative proteomics analysis.Results: A dramatic decrease of ATP4B expression in GC cells and tissues compared with the adjacent normal tissues was observed; Downexpression of ATP4B was associated with the malignant transformation in gastric mucosa lesions and correlated with poor prognosis, high grade of TNM stage, and vessel carcinoma embolus in GC patients. Restoration of ATP4B expression in GC cells significantly inhibited cell proliferation, cell viability, migration, invasion, tumorigenicity and induced apoptosis, whereas ATP4B silencing exerted the opposite effects. Mechanistically, we found a constitutive activation of p53 and inactivation of NF-κB signaling correlated with the mitochondrial pathway in ATP4B-overexpressing GC cells.Conclusion: Our data suggest that ATP4B perform the promising tumor suppressor gene by regulating p53/NF-κB/mitochondrial pathway in GC.

Transcription factor NF-κB participates in regulation of epithelial cell turnover in the colon

2000 ◽  
Vol 279 (6) ◽  
pp. G1282-G1291 ◽  
Author(s):  
Mehmet Sait Inan ◽  
Veronica Tolmacheva ◽  
Qiang-Shu Wang ◽  
Daniel W. Rosenberg ◽  
Charles Giardina
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Transcription Factor ◽  
Epithelial Cell ◽  
Dna Binding ◽  
Epithelial Cells ◽  
Knockout Mice ◽  
Nuclear Antigen ◽  
Cell Turnover ◽  
Tnf Α

The transcription factor nuclear factor (NF)-κB regulates the expression of genes that can influence cell proliferation and death. Here we analyze the contribution of NF-κB to the regulation of epithelial cell turnover in the colon. Immunohistochemical, immunoblot, and DNA binding analyses indicate that NF-κB complexes change as colonocytes mature: p65-p50 complexes predominate in proliferating epithelial cells of the colon, whereas the p50-p50 dimer is prevalent in mature epithelial cells. NF-κB1 (p50) knockout mice were used to study the role of NF-κB in regulating epithelial cell turnover. Knockout animals lacked detectable NF-κB DNA binding activity in isolated epithelial cells and had significantly longer crypts with a more extensive proliferative zone than their wild-type counterparts (as determined by proliferating cell nuclear antigen staining and in vivo bromodeoxyuridine labeling). Gene expression profiling reveals that the NF-κB1 knockout mice express the potentially growth-enhancing tumor necrosis factor (TNF)-α and nerve growth factor-α genes at elevated levels, with in situ hybridization localizing some of the TNF-α expression to epithelial cells. TNF-α is NF-κB regulated, and its upregulation in NF-κB1 knockouts may result from an alleviation of p50-p50 repression. NF-κB complexes may therefore influence cell proliferation in the colon through their ability to selectively activate and/or repress gene expression.

Association of Tumor Cell Proliferation with Lymph Node Metastasis in Early Gastric Cancer

Oncology ◽  
1996 ◽  
Vol 53 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Kiyoshi Maeda ◽  
Yong-Suk Chung ◽  
Naoyoshi Onoda ◽  
Masafumi Ogawa ◽  
Yasuyuki Kato ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Early Gastric Cancer ◽  
Tumor Cell ◽  
Tumor Cell Proliferation ◽  
Node Metastasis

scholarly journals Surrounding Gastric Mucosa Findings Facilitate Diagnosis of Gastric Neoplasm as Gastric Adenoma or Early Gastric Cancer

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Tadashi Miike ◽  
Shojiro Yamamoto ◽  
Yoshifumi Miyata ◽  
Tomoya Hirata ◽  
Yuko Noda ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Mucosa ◽  
Early Gastric Cancer ◽  
Atrophic Gastritis ◽  
Narrow Band Imaging ◽  
Gastric Neoplasm ◽  
Gastric Neoplasms ◽  
Gastric Adenoma ◽  
Open Type ◽  
Gastric Cancers

Background and Aim. It is difficult to master the skill of discriminating gastric adenoma from early gastric cancer by conventional endoscopy or magnifying endoscopy combined with narrow-band imaging, because the colors and morphologies of these neoplasms are occasionally similar. We focused on the surrounding gastric mucosa findings in order to determine how to discriminate between early gastric cancer and gastric adenoma by analyzing the characteristics of the gastric background mucosa.Methods. We retrospectively examined 146 patients who underwent endoscopic submucosal dissection for gastric neoplasm between October 2009 and January 2015. The boundary of atrophic gastritis was classified endoscopically according to the Kimura-Takemoto classification system. Of 146 lesions, 63 early gastric cancers and 21 gastric adenomas were ultimately evaluated and assessed.Results. Almost all gastric adenomas were accompanied by open-type gastritis, whereas 47 and 16 early gastric cancers were accompanied by open-type and closed-type gastritis, respectively (p= 0.037).Conclusions. The evaluation of the boundary of atrophic gastritis associated with gastric neoplasms appears to be useful for discrimination between early gastric cancer and gastric adenoma. When gastric neoplasm is present in the context of surrounding localized gastric atrophy, gastric cancer is probable but not certain.

scholarly journals CELL PROLIFERATION OF EARLY GASTRIC CANCER

1968 ◽  
Vol 18 (2) ◽  
pp. 275-286
Author(s):  
KOJIRO SHICHIJO ◽  
HIROSHI SUTO ◽  
JIRO KOBAYASHI ◽  
ICHIRO OOKI ◽  
MINORU TUKADA ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Early Gastric Cancer
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