Artifactual changes in hematological variables in equine blood samples stored at different temperatures and various anticoagulants

2010 ◽  
Vol 21 (4) ◽  
pp. 449-452 ◽  
Author(s):  
Maysam Tehrani Sharif ◽  
Mehrdad Ameri Mahabadi ◽  
Sogand Moshfeghi ◽  
Hamid Sharifi ◽  
Seyed Mohammad Hoseini ◽  
...  
Keyword(s):  
Blood Samples ◽  
Different Temperatures ◽  
Artifactual Changes

Artifactual changes in hematological variables in equine blood samples stored at different temperatures and various anticoagulants

2010 ◽  
Author(s):  
Maysam Tehrani-Sharif ◽  
Mehrdad Ameri ◽  
Sogand Moshfeghi ◽  
Hamid Sharifi ◽  
Seyed Mohammad Hoseini ◽  
...  
Keyword(s):  
Blood Samples ◽  
Different Temperatures ◽  
Artifactual Changes

Effects of some pre-analytical conditions on the measurement of homocysteine and cysteine in plasma

2004 ◽  
Vol 42 (2) ◽  
Author(s):  
Maddalena L. Zighetti ◽  
Veena Chantarangkul ◽  
Rossana Lombardi ◽  
Anna Lecchi ◽  
Marco Cattaneo
Keyword(s):  
Venous Thrombosis ◽  
Room Temperature ◽  
Reliable Measurement ◽  
Blood Samples ◽  
Artifactual Changes ◽  
Total Homocysteine ◽  
Citrate Dextrose ◽  
Crushed Ice ◽  
Plasma Total Homocysteine

AbstractThe association of hyperhomocysteinemia and hypercysteinemia with the risk of arterial and venous thrombosis is well documented. While it is known that standardized pre-analytical conditions are necessary for reliable measurement of plasma total homocysteine, the effects of pre-analytical conditions on cysteine measurement are less well known. The aim of this study was to evaluate the effects of pre-analytical conditions on the measurement of homocysteine and cysteine. We observed that the concentration of total homocysteine in plasma increased significantly with time (38% after 6 h), whereas total cysteine decreased (5% after 2h) when blood anticoagulated with ethylenediaminetetraacetic tripotassium salt was kept at room temperature. These changes were minimized when acidic citrate dextrose was used as an anticoagulant and were abolished when blood samples were immediately placed on crushed ice, independently of the anticoagulant. Storage of plasma for 72h at room temperature induced a small (≅6%), but significant, decrease in cysteine when blood was collected in ethylenediaminetetraacetic tripotassium salt. In contrast, homocysteine was stable in plasma for 72h, independently of the anticoagulant used. In conclusion, if blood samples for plasma total homocysteine and cysteine measurement cannot be kept on ice, they should be collected in acidic citrate dextrose to minimize the artifactual changes.

scholarly journals The Impact of Dehydration and Hyperthermia on Circulatory Glutathione Metabolism after Exercise in the Heat with Insights into the Role of Erythrocytes

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1144
Author(s):  
Denise de Melo-Marins ◽  
Juliano Boufleur Farinha ◽  
Franccesco Pinto Boeno ◽  
Alexandra Ferreira Vieira ◽  
Samuel Vargas Munhoz ◽  
...  
Keyword(s):  
Heat Stress ◽  
Redox Balance ◽  
Water Bath ◽  
Glutathione Metabolism ◽  
Blood Samples ◽  
Before And After ◽  
Different Temperatures ◽  
The Impact

Background: Reduced glutathione (GSH) is one of the main thiols involved in antioxidant defense. Changes in circulatory levels of GSH during exercise are associated with hyperthermia and dehydration. The mechanisms by which these alterations occur are not entirely known. We hypothesize that erythrocytes could be an important source of circulatory GSH during heat stress conditions. We performed two separate experiments to address this hypothesis. Methods: In the first experiment, we sought to investigate the impact of exercise in the heat and dehydration on erythrocyte levels of GSH. A total of 10 men performed 60 min of cycling at 60% VO2peak in the heat (38.0 ± 0.9 °C) or in a control temperate environment (23.0 ± 1.0 °C), both with and without dehydration. Relative humidity ranged from 50 to 70%. Blood samples were taken before and after exercise to measure GSH and oxidized (GSSG) glutathione. In the second experiment, erythrocytes were isolated from blood samples taken at rest and heated in vitro to determine the impact of heat on erythrocyte glutathione content. Tubes with erythrocytes were exposed to water baths at different temperatures; one tube was exposed to a water bath at 35 °C and the other tube to a water bath at 41 °C for a period of 30 min. After exposure to heat, plasma and erythrocytes were extracted for GSH and GSSG analyses. Results: Dehydration decreased circulatory GSH, regardless of ambient temperature (temperate and heat decreased 15.35% and 30.31%, respectively), resulting in an altered redox balance. Heat increased GSH levels in vitro. Conclusion: Our data suggest that dehydration decreases circulatory GSH levels regardless of environmental temperature. In addition, in vitro data suggests that erythrocytes may contribute to the release of GSH during exposure to heat stress.

scholarly journals Stability of Ethanol in Blood and Urine Samples

2007 ◽  
Vol 26 (3) ◽  
pp. 241-244 ◽  
Author(s):  
Slavka Mandić-Radić ◽  
Gordana Džingalašević ◽  
Nevena Luković
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Urine Samples ◽  
Time Intervals ◽  
Blood Samples ◽  
Duration Of Storage ◽  
Different Temperatures ◽  
Blood And Urine Samples ◽  
Excessive Alcohol ◽  
Temperature Storage

Stability of Ethanol in Blood and Urine SamplesThe changes of ethanol concentrations in whole blood and urine samples were analyzed depending on temperatures and duration of storage. The aim of the study was to establish standards for the Institute laboratory. Samples of whole blood and urine, taken from drivers with excessive alcohol concentrations (6 groups, 15 samples per each), were analyzed upon delivery and then after storage during different time intervals and at different temperatures. The results showed that alcohol concentrations were significantly reduced with the increase of temperature and prolongation of storage. Only the whole blood samples stored for up to one month at -20 °C did not show significant changes. Room temperature storage of samples is the least suitable way of keeping them, independently of the duration of storage. Urines are not less reliable samples than blood. There are no ethanol differences between blood samples with and without sodium fluoride.

Effects of time and temperature on 48 routine chemistry, haematology and coagulation analytes in whole blood samples

2016 ◽  
Vol 54 (4) ◽  
pp. 448-462 ◽  
Author(s):  
Jasmijn A van Balveren ◽  
Mirelle JAJ Huijskens ◽  
Eugenie FA Gemen ◽  
Nathalie CV Péquériaux ◽  
Ron Kusters
Keyword(s):  
Whole Blood ◽  
Ethylenediaminetetraacetic Acid ◽  
Blood Analysis ◽  
Blood Samples ◽  
Total Allowable Error ◽  
Different Temperatures ◽  
Allowable Error ◽  
Whole Blood Samples ◽  
Almost All ◽  
Donated Blood

Background Phlebotomy for the purpose of blood analysis is often performed at remote locations, and samples are usually temporarily stored before transport to a central laboratory for analysis. The circumstances during storage and shipment may not meet the necessary requirements. If analysed anyway, false results may be generated. We therefore examined the influence of precentrifugation time and temperature of the most frequently requested tests in whole blood. Methods Healthy volunteers donated blood in which 48 analytes were tested. Routine chemistry was performed in lithium heparin tubes, haematology in ethylenediaminetetraacetic acid tubes, coagulation in citrate tubes and glucose in sodium fluoride tubes. One tube was measured directly. The others were kept at different temperatures (4, 8, 20 or 30℃) and stored for 4, 6, 8 or 24 h before analysis. Additionally, some analytes were examined at 12, 16, 24 and 28℃. The mean percentage deviation was compared with different decision levels, including the total allowable error. Results When using the total allowable error as an acceptable limit, most of the investigated analytes remained stable. However, bicarbonate is unstable at almost all tested time-points and temperatures. Calcium, lactate dehydrogenase, potassium and sodium are particularly affected at low temperatures, while phosphate is mainly affected at and above room temperature after 8 h. Conclusion We established the influence of time and temperature on a broad range of analytes, which may be applied to set the limits in transportation and storage of whole blood samples.

Observations oh Nucleation and Growth of Voids in Nickel

1970 ◽  
Vol 28 ◽  
pp. 414-415
Author(s):  
J. L. Brimhall ◽  
H. E. Kissinger ◽  
B. Mastel
Keyword(s):  
High Purity ◽  
Growth Stage ◽  
Elevated Temperatures ◽  
Early Growth ◽  
Nucleation And Growth ◽  
Pure Nickel ◽  
Different Temperatures ◽  
Total Fluence ◽  
Neutron Exposure ◽  
Growth Of Voids

Some information on the size and density of voids that develop in several high purity metals and alloys during irradiation with neutrons at elevated temperatures has been reported as a function of irradiation parameters. An area of particular interest is the nucleation and early growth stage of voids. It is the purpose of this paper to describe the microstructure in high purity nickel after irradiation to a very low but constant neutron exposure at three different temperatures.Annealed specimens of 99-997% pure nickel in the form of foils 75μ thick were irradiated in a capsule to a total fluence of 2.2 × 1019 n/cm2 (E > 1.0 MeV). The capsule consisted of three temperature zones maintained by heaters and monitored by thermocouples at 350, 400, and 450°C, respectively. The temperature was automatically dropped to 60°C while the reactor was down.

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