Influence of storage at different temperatures on inorganic phosphorus levels of cattle blood samples

1982 ◽  
Vol 111 (19) ◽  
pp. 441-441 ◽  
Author(s):  
A. Hunter ◽  
K. Matthews
1970 ◽  
Vol 42 (1) ◽  
pp. 8-20
Author(s):  
Vappu Kossila ◽  
Paavo Niemelä ◽  
Erkki Koskenkorva

Blood mineral studies were carried out in the dairy herd of the Viik Experimental Farm, in which parturient paresis has been successfully prevented by intramuscular injections of vitamin D3 (2 X 5 million I.U.). The material consisted of 41 cows from which 207 blood samples had been taken in 1963 and 12 cows (6 controls, 6 vitamin D injected cows) from which 72 blood samples had been taken in 1969. All cows were either dry and pregnant or lactating and nonpregnant. Compared to values obtained during the dry period, the serum calcium level was only slightly lower shortly after calving. The calcium level increased in the vitamin D treated cows during the ascending phase of lactation, whereas both the calcium and inorganic phosphorus levels remained relatively low in the control cows, respectively. The highest mean magnesium levels occurred during the 2nd and 3rd weeks of lactation. The serum calcium level was higher during the grazing period than during the indoor feeding period. Inorganic phosphorus remained at a relatively high level in younger cows during the grazing period, whereas a significant decrease was noted in the phosphorus level of older cows, respectively. The lowest magnesium values were noted in May, i.e. already before the beginning of the pasture season, the concentration increasing towards the end of the grazing period especially in younger cows. As a whole, younger cows had slightly higher mean serum calcium and magnesium levels and significantly higher inorganic phosphorus levels compared to older cows. The effect of age on the inorg. phosphorus level appeared to be more marked in dry cows and during the grazing period than in lactating cows and during the winter feeding period. Special attention has been paid to the blood mineral values of four individual cows suffering from various illnesses during the study period.


1930 ◽  
Vol 3 (2) ◽  
pp. 120-124 ◽  
Author(s):  
John Allardyce ◽  
R. H. Fleming ◽  
F. L. Fowler ◽  
R. H. Clark

Samples of cattle blood have been analysed and the concentrations of 10 constituents determined, i.e., cholesterol, sugar, non-protein nitrogen, urea nitrogen, amino-acid nitrogen, creatine, creatinine, calcium, inorganic phosphorus and chlorides. The normal range of each of these constituents was established. The diet fed to the cattle influences the cholesterol content markedly. Blood samples of cattle suffering from red water and some other pathological cases have been examined, and in each case the results show the variation of the contents of the various constituents.


1960 ◽  
Vol 54 (2) ◽  
pp. 235-242 ◽  
Author(s):  
D. H. L. Rollinson ◽  
R. M. Bredon

Studies have been made on calcium and inorganic phosphorus in pasture grass and their relationship to blood levels in Zebu cattle.Excitement, adrenaline injections and fatigue were investigated as possible causes of variations in the inorganic-phosphorus levels of the blood of Zebu cattle.Sudden rainfall appeared to cause alterations in the inorganic phosphorus levels of blood. This hypothesis, when tested on two separate batches of cattle on two occasions, showed that there was a highly significant relationship between water intake and the level of inorganic phosphorus of Zebu cattle blood.Water starvation caused a steady increase in the level of inorganic phosphorus and when water was available for the cattle to drink the inorganic phosphorus level fell rapidly to a subnormal level before returning to normal. These results are discussed in the light of the available literature.


2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Mohammad E. Naffaa ◽  
Mona Mustafa ◽  
Mohje Azzam ◽  
Roni Nasser ◽  
Nizar Andria ◽  
...  

2019 ◽  
Vol 6 (2) ◽  
pp. 232
Author(s):  
Sutikno Sutikno ◽  
Rudy Priyanto ◽  
Cece Sumantri ◽  
Jakaria Jakaria

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida  g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.


Author(s):  
N. O. Kravchenko ◽  
L. V. Kovalenko ◽  
O. P. Rudenko ◽  
V. S. Boiko

The purpose of these studies was to determine status of metabolic processes in clinically healthy horses of sport breeds in spring period. Blood samples for biochemical studies were collected from 12 clinically healthy 7–9 month-old stallions of Ukrainian horse breed at Dnipropetrovsk region equestrian club. Protein (albumin, globulin, urea and creatinine) and mineral (common calcium and inorganic phosphorus) metabolic statuses, level of glucose, vitamins A and E and acid, as well as activity of hepatospecific enzymes (ALT, AST and AP) were determined using common techniques. It has been found that common protein level was within the limits of physiologic norm, although the level of albumins was decreased at the average rate of 12.0%, and the level of β- and γ-globulins was increased at the average rates of 5.2 and 11.3% respectively. AST activity was decreased at the rate of 38.0% regarding to physiological norm. Thereby, urea and creatinine concentrations were within the referent levels. Hyperglycaemia was observed in 50.0% of tested animals with maximal excess at the rate of 44.0%. Also, decreasing of common calcium and inorganic phosphorus levels was determined at the rate of 16.0 and 58.6%, vitamins А and Е — at the rate of 64.0 and 48,6% respectively, in comparison to lower level of physiological norm. The average index of acid capacity reached maximal referent levels. At the same time, it was increased in 33.3% of animals. Therefore, detected changes in biochemical indices in horse blood evidence that various metabolic disorders progress in clinically healthy stallions at spring and may furtherly lead to the appearance of metabolic syndrome


2018 ◽  
Vol 43 (4) ◽  
pp. 323
Author(s):  
S. Sutikno ◽  
R. Priyanto ◽  
C. Sumantri ◽  
J. Jakaria

The ADIPOQ and EDG1 genes were responsible in intramuscular fat deposition and marbling scores. This study was aimed to identify polymorphism of indel g.81966364D>I in promoter region of ADIPOQ gene and SNP c.-312A>G in 5' UTR of EDG1 gene in Indonesian beef cattle. Blood samples were collected from 211 cattle, including Bali (44), Madura (20), Pesisir (18), Katingan (20), PO (22), Pasundan (20), SO (12), Brahman (20), Simmental (15) and Limousin (18). Polymorphism of ADIPOQ gene was analyzed using PCR and direct sequencingmethods, whereas EDG1 gene was analyzed using PCR-RFLP (MscI enzyme) and direct sequencingmethods. Results of genotyping indel g.81966364D>I was monomorphic (DD genotype). The SNP c.-312A>G was polymorphic (AA and AG genotype) in Madura, Pesisir, Pasundan, Brahman, and Limousine. The Frequencies of allele A and G were 0.95, 0.92, 0.98, 0.95, 0.94 and 0.05, 0.08, 0.02, 0.05, 0.06 respectively. The values of Ho and He were 0.05-0.17 and 0.05-0.15 respectively and in Hardy-Weinberg equilibrium (P>0.05). In Bali, Katingan, PO, SO and Simmental were monomorphic (GG genotype). In Bali cattle, two novel SNP candidates were found in position of c.-399C>T and c.-273C>G which were potential to be used as genetic markers of marbling score for Bali cattle. As result this study, it can be concluded that ADIPOQ gene was similar while EDG1 gene was different in Indonesian beef cattle. in addition, found two candidates potential SNP in Bali cattle.


1973 ◽  
Vol 13 (64) ◽  
pp. 537 ◽  
Author(s):  
RF Thornton ◽  
DJ Minson

During the concluding phase of a six-year grazing trial a study was made of the steers and the pasture material from paddocks annually fertilized with 125 kg ha-1and 250 kg ha-1of superphosphate. Plucked pasture samples from the low and high superphosphate treatments contained 0.11 and 0.15 per cent phosphorus, 0.83 and 0.91 per cent nitrogen and 0.12 and 0.12 per cent sulphur, respectively. The N: S ratio of these samples were 6.8 and 7.6 and the in vitro digestibilities were 41.6 and 44.9 per cent. There were marked seasonal patterns in the concentrations of all components with minimum values in the winter months when liveweight gain was lowest. Greater superphosphate application increased mean liveweight gain (0.29 to 0.41 kg head-1day-1), mean plasma concentrations of both urea-nitrogen (6.3 vs 5.0 mg 100 ml-1) and of inorganic phosphorus (6.7 vs 6.3 mg 100 ml-1). The mean blood sulphate-sulphur concentration of 28 �g ml-I was not influenced by treatment. Comparisons of the levels of nutrients in pasture and blood with published requirements indicated that neither sulphur nor phosphorus were deficient in either treatment. However, the between treatment differences in both nitrogen and phosphorus levels could not be ignored as possible reasons for the difference in animal production between the two levels of superphosphate application. Improved animal performance associated with increased application of superphosphate was probably influenced more by the induced changes in the organic components rather than the mineral levels of the pasture material.


Author(s):  
Maysam Tehrani-Sharif ◽  
Mehrdad Ameri ◽  
Sogand Moshfeghi ◽  
Hamid Sharifi ◽  
Seyed Mohammad Hoseini ◽  
...  

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