A peptide nucleic acid–regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles

2020 ◽  
Vol 187 (7) ◽  
Author(s):  
Tingting Gao ◽  
Shu Xing ◽  
Mengjia Xu ◽  
Pan Fu ◽  
Jiechen Yao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Energy Transfer ◽  
Nucleic Acid ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Peptide Nucleic Acid ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Assay ◽  
Fluorescence Resonance

Fluorescence resonance energy transfer monitoring of pH-responsive doxorubicin release from carbon dots/aptamer functionalized magnetic mesoporous silica

Nanomedicine ◽  
2021 ◽  
Author(s):  
Abolghasem Abbasi Kajani ◽  
Masoud Ayatollahi Mehrgardi
Keyword(s):  
Drug Delivery ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Drug Loading ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Techniques ◽  
Ph Responsive ◽  
Fluorescence Resonance

Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculated surface area of 552.83 m2.g-1, high magnetic activity (12.6 emu.g-1), appropriate colloidal stability and high drug loading capacity (up to 61%). Conclusion: Taxane-based carbon dots act as the pH-responsive gatekeepers for the controlled release of doxorubicin into cancer cells and provide a fluorescence resonance energy transfer system for real-time monitoring of drug delivery.

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