Molecular analysis of population structure and antibiotic resistance of Klebsiella isolates from a three-year surveillance program in Florence hospitals, Italy

2011 ◽  
Vol 31 (3) ◽  
pp. 371-378 ◽  
Author(s):  
F. Donnarumma ◽  
C. Indorato ◽  
G. Mastromei ◽  
E. Goti ◽  
P. Nicoletti ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Population Structure ◽  
Molecular Analysis ◽  
Surveillance Program

Molecular analysis of genetic diversity, population structure, and phylogeny of wild and cultivated tulips (Tulipa L.) by genic microsatellites

2018 ◽  
Vol 59 (6) ◽  
pp. 875-888 ◽  
Author(s):  
Ali Pourkhaloee ◽  
Morteza Khosh-Khui ◽  
Paul Arens ◽  
Hassan Salehi ◽  
Hooman Razi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Analysis ◽  
Genic Microsatellites

Distribution of TET, AAC and CTX-M Genes among Antibiotic Resistant Escherichia coli Isolated From Poultry under Various Farming System of A and N Islands

2021 ◽  
Author(s):  
Jai Sunder ◽  
T. Sujatha ◽  
S. Bhowmick ◽  
S.C. Mayuri ◽  
A.K. De ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Farming Systems ◽  
Farming System ◽  
Multi Drug Resistance ◽  
Surveillance Program ◽  
Poultry Production ◽  
Animal Food ◽  
E Coli

Background: Transmission of antibiotic resistance from animal food chain to human through animal food-borne pathogens have led to increased public concern. Wider surveillance on prevalence of antibiotic resistance in E. coli will provide information on evolution of resistance in various geographical locations. The purpose of this study was to investigate the presence of antimicrobial resistance of E. coli isolates from poultry under various farming system in A and N Islands and resistance genes of tet, ctx-M and aac encoding the isolates. Methods: Isolates were obtained from cloacal swabs in poultry under various farming systems and tested against major antimicrobial derivatives to study multi drug resistance. The presence of genes associated with resistance to tetracycline (tet A), ESBL (CTX-M) and Gentamycin (aac(3)-IV) were determined by PCR. Result: A total of 126 cloacal samples were analysed out of which 31.38% of the E.coli isolates from poultry under various farming systems were producing extended spectrum beta-lactamases and were multiple antimicrobial resistant. Poultry birds of commercial farms showed higher resistance levels (37.5%) than organised farms (24.76%) and desi birds (31.88%). Results indicate a high level of multi-drug resistance is emerging even in desi birds. It is suggested that an antimicrobial resistance surveillance program is needed in A and N Islands in order to detect bacterial resistance among rural poultry production as the 80 percentage of total poultry population belong to desi birds.

Molecular analysis of genetic diversity, population structure and inbreeding level of the Italian Lipizzan horse

2013 ◽  
Vol 151 (2-3) ◽  
pp. 124-133 ◽  
Author(s):  
Gianni Barcaccia ◽  
Michela Felicetti ◽  
Giulio Galla ◽  
Stefano Capomaccio ◽  
Katia Cappelli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Analysis ◽  
Inbreeding Level

scholarly journals How to Deal with Antibiotic Resistance?

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Bilal H ◽  
◽  
Wang L ◽  
Khan MN ◽  
Yang X ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Current Situation ◽  
Surveillance Study ◽  
Surveillance Program ◽  
Prevalence Rates ◽  
Global Problem ◽  
High Prevalence ◽  
Study Programs ◽  
Comprehensive Surveillance

Antibiotic resistance is the most serious global problem to be addressed. This requires comprehensive surveillance study programs at the level of countries, especially those with high prevalence rates. Therefore, we have tried to point out the missing gaps of Pakistan’s antibiotic resistance surveillance study programs in the light of our previously published article. We hope this will help to improve the current situation of antibiotic resistance and the existing surveillance program.

scholarly journals Temporal Dynamics and Impact of Manure Storage on Antibiotic Resistance Patterns and Population Structure of Escherichia coli Isolates from a Commercial Swine Farm

2007 ◽  
Vol 73 (17) ◽  
pp. 5486-5493 ◽  
Author(s):  
Patrick Duriez ◽  
Edward Topp
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Population Structure ◽  
Temporal Dynamics ◽  
Swine Farm ◽  
Bacterial Populations ◽  
E Coli ◽  
Resistance Patterns ◽  
Fresh Feces ◽  
Antibiotic Resistance Patterns

ABSTRACT Many confined-livestock farms store their wastes for several months prior to use as a fertilizer. Storing manure for extended periods could significantly bias the composition of enteric bacterial populations subsequently released into the environment. Here, we compared populations of Escherichia coli isolated from fresh feces and from the manure-holding tank (stored manure) of a commercial swine farm, each sampled monthly for 6 months. The 4,668 confirmed E. coli isolates were evaluated for resistance to amikacin, ampicillin, cephalothin, chloramphenicol, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline, trimethoprim, and trimethoprim plus sulfamethoxazole. A subset of 1,687 isolates was fingerprinted by repetitive extragenic palindromic PCR (rep-PCR) with the BOXA1R primer to evaluate the diversity and the population structure of the collection. The population in the stored manure was generally more diverse than that in the fresh feces. Half of the genotypes detected in the stored manure were never detected in the fresh fecal material, and only 16% were detected only in the fresh feces. But the majority of the isolates (84%) were assigned to the 34% of genotypes shared between the two environments. The structure of the E. coli population showed important monthly variations both in the extent and distribution of the diversity of the observed genotypes. The frequency of detection of resistance to specific antibiotics was not significantly different between the two collections and varied importantly between monthly samples. Resistance to multiple antibiotics was much more temporally dynamic in the fresh feces than in the stored manure. There was no relationship between the distribution of rep-PCR fingerprints and the distribution of antibiotic resistance profiles, suggesting that specific antibiotic resistance determinants were dynamically distributed within the population.

Sign in / Sign up

Export Citation Format

Share Document