Review on Epidemiology, Control and Public Health Importance of Bovine Fasciolosis

Fascioliasis is a trematode zoonotic snail-borne disease of public health and economic importance. Fasciolosis is a parasitic disease that affects most population of cattle and it exists in almost all parts of the world. It is caused by commonly known species of liver fluke that are Fasciola hepatica and Fasciola gigantica, which mainly affects domestic ruminants. Fasciolosis is more apparent in young cattle and is usually chronic in nature. Adult flukes in the bile ducts cause inflammation, biliary obstruction, distraction of liver tissue and anemia. Fasciolosis is the major burden for Animal production and by direct or indirect economic losses at different part of our country. Diagnosis of fasciolosis is based primarily on clinical signs and seasonal occurrence in endemic areas but previous history of fasciolosis on the farm or identification of snail habitats; postmortem examinations, hematological tests and examination of faeces for fluke eggs are useful. The life cycle of Fasciola spp. is a typical of digenetic treamatodes. Eggs laid by the adult parasite in the bile ducts of their hosts pass into the duodenum with the bile. The life cycle of Fasciola goes through the intermediate host and several developmental stages in the intermediate host. On a herd basis, clinical signs of fluke infection are usually vague (i.e., reduced productivity) and can be difficult to differentiate from the effects of less-thanoptimal management or other chronic disease conditions. Transmission of fasciolosis infection is depending on the presence of “lymnea snail”, host and final host. Among many parasitic problems of farm animals, fasciolosis is a major disease which imposes economic impact on livestock production particularly of cattle and sheep and it has public health importance as it have zoonotic value.

Epidemiology of Blackleg Disease in Ethiopia

Blackleg is an infectious bacterial disease of cattle and rarely of other ruminants. This bacteria is caused by Clostridium chauvoei which is an anaerobic, gram positive, motile, rod-shaped bacillus bacterium and persists in the soil as resistant spores. Blackleg is an acute or subacute but chronic disease may occur. It occurs most frequently in animals 6-24 months of age and the disease mainly affects none vaccinated as well as animals in good nutritional condition. It produces persistent spores when conditions are not ideal and spores are highly resistant to environmental factors and disinfectants. Infected ruminants do not directly transmit the disease to other animals. The bacteria enter the body through the alimentary mucosa after ingestion of contaminated feed. Secretion of cytolytic toxins that cause necrosis of vascular endothelia .The toxins are absorbed into the animal’s bloodstream which makes the animal acutely sick and causes rapid death. Economic importance due to blackleg is loss of animals, milk production and draft oxen, and cost for treatment and vaccination. Fatality rate of blackleg in fully susceptible populations approaches 100%. Clinical Signs include lethargy anorexia, reluctance to move lameness and recumbence. When superficial muscles are affected, swelling and crepitus are evident. Cattle found dead of blackleg are lying on the side with the affected hind limb stands out stiffly, bloating and putrefaction occur quickly and bloodstained from exudates, nostrils and anus. The disease can be diagnosed using laboratory diagnosis, Immune Fluorescent, Cell Culture and PCR. Control and prevention relies mainly on vaccination.

Sero Status and Presumed Risk Factors Assessment for Bovine Herpesvirus-1 in North Western, Ethiopia

A cross sectional study was conducted from November 2020 to June 2021 on a total of 442 randomly selected indigenous zebus (bos indicus) of extensively and semi-intensively managed cattle’s. These cattle’s were sampled from East Gojam (n=137), west Gojam (n=160) and Awi zone (n=145) north western part of Ethiopia. Indirect Enzyme-Linked Immune-Sorbent Assay (I-ELISA) was used to detect antibodies specific to Bovine Herpes Virus-1 (BoHV-1). The relationship of the categories under the variables was analyzed using a chisquare descriptive statistic. Logistic regression analyses were also used to assess potential predictive factors associated with the outcome variable. An overall animal level sero-prevalence of BoHV-1 antibodies were 77.6% (95% CI: 73.5-81.3%) for the present study. In a random-effects of an adjusted logistic regression model, geographically the sero-prevalence of BoHV-1 exposure was higher for cattle’s from East Gojam (Odds ratio [OR] =0.2; p=0.002) than in Awi zone (OR=1.4; p=0.377) and West Gojam. The likelihood of disease occurrence for introduced cattle’s were relatively higher (OR=0.4; p= 0.001) than homebred. Age of cattle’s and parity status of heifers/cows were significantly associated with BoHV-1 infection (p<0.001). The risk of infection was also positively correlated with the occurrence of respiratory problem (OR=2.2; P=0.048). Thus, this result signifies that BoHV-1 is widely circulating among the indigenous zebus in the study areas. Given that there was no BoHV-1 vaccination delivery in the study area and in the country as a whole, control and prevention measures using marker vaccines were highly recommended.

A Review on Veterinary Drug Management, Handling, Utilization and Its Resistance Side Effects

Veterinary drugs play an important role in the control and prevention of animal diseases and animal sufferings, however it have the potential to cause harm if it is not used properly. Successful animal health service provision needs the availability of safe, effective and affordable drugs of the required quality and quantity. The available drugs must be prescribed, presented, transported, dispensed, labeled, packaged, measured and used rationally. Rational use of drugs in veterinary medicine has manifold significance; it can be either public health significance, improve food safety concern, reduce the development of drug resistance and residue or economic significance; the need to rely on more expensive drugs and international trade barrier. The potential public health hazard as a result of irrational drug use in food animals includes limited efficacy, increase risk of unwanted effects such as the emergence of drug resistance and drug residue, waste of resources and psychosocial impacts. Irrational drug using is characterized by over-prescription, inappropriate dosage, incorrect duration and unnecessary risk. There are several reasons which may contribute to irrational use of drugs. Some of them are lack of information, inadequate training and education of graduates of veterinary medicine, poor communication between veterinarian and owner, lack of diagnostic facilities, demand from the animal owner, and promotional activities of pharmaceutical industries. This paper had induced to document and aware procedures and necessary activities to have rational drug use, and it would helps to have proper drug measurement and dose calculation as well as it tell us about 0drug resistance and its public health effects. Over all This review hoped that as it would be help to animal health professionals and enhance the quality of veterinary pharmaceutical services so that decrease the economic loss and public health hazard due to diseases of livestock and protects the impacts of drug resistance and its residue.

Freezability of Goat Epididymal Sperm using Aloe vera Extract and Trehalose in Diluents

Background: Cryopreservation process causes oxidative stress on sperm membranes, which in turn damages sperm organs and enzymatic activities which thereby decrease motility, functional membrane integrity and sperm fertility. Therefore, current study carried out to evaluate the effect of Aloe vera Ethanolic Extract (AEE), alone and with trehalose in diluents on cryopreserved epididymal goat sperm. Methodology: Epididymal sperm isolated from testes with motility >70% and total morphological abnormalities <10%. The experimental treatments consist of control (no additives) and basic diluents plus 5, 10, 20 or 50 μg/ml of AEE (AEE1, AEE2, AEE3 and AEE4, respectively), tr (150 mM trehalose), tr+AEE1, tr+AEE2, tr+AEE3 and tr+AEE4. Results: Obtained data show that the extender containing AEE3, AEE1+tr, AEE2+tr and AEE3+tr improved significantly the cryopreserved sperm. The combined treatments indicate also a decrease in MDA than control. In addition, AEE2+tr and AEE3+tr showed the lowest (P<0.05) DNA fragmentation compared to the other treatments. Extender containing AEE3+tr resulted in higher total motility and viability than the extender containing tr alone, as well as AEE1, AEE2 and AEE4 treatments. Conclusion: The present study indicates that ethanolic extract of Aloe vera could be used for goat sperm cryopreservation. Also, it can be concluded that trehalose in combination with 20 μg/ml of Aloe vera extract can be promised cryoprotectant in goat epididymal sperm freezing.

How to Deal with Antibiotic Resistance?

Antibiotic resistance is the most serious global problem to be addressed. This requires comprehensive surveillance study programs at the level of countries, especially those with high prevalence rates. Therefore, we have tried to point out the missing gaps of Pakistan’s antibiotic resistance surveillance study programs in the light of our previously published article. We hope this will help to improve the current situation of antibiotic resistance and the existing surveillance program.

Knockdown of ANGPTL4 May Reduce the Expression of LPL during Differentiation of 3T3-L1 Preadipocyte

Adipose tissue is a complex, highly metabolically active tissue. The expansion of adipose tissue is mainly affected by two processes: the number of adipocytes and the change of adipocyte size. Adipocyte differentiation is regulated by multiple factors. 3T3-L1 preadipocyte line has become the most widely studied cell line for adipocyte proliferation, differentiation and regulation of related genes. In our previous study, we found that ANGPTL4 was related to preadipocyte differentiation through PPAR signaling pathway. Recent studies have shown that ANGPTL4 has functions of regulating oncogenesis, vascular permeability, glucose homeostasis, lipid metabolism, energy homeostasis. In this study, we observed the changes of key genes expression before and after inhibiting ANGPTL4 to explore how ANGPTL4 influence on lipid metabolism. Results shows that, ANGPTL4 and LPL have similar expression pattern. We suspect that, ANGPTL4 and LPL may form a pathway, when ANGPTL4 is suppressed it may block the pathway and inhibit LPL.

Effect of Liposome Curcumin Combined with PDGF-BB on Tendon Healing in Rats

Objective: To study the effect of liposomal curcumin combined with PDGFBB on tendon healing after tendon injury in rats and explore its mechanism. Methods Thirty-six rats were randomly divided into four groups: healthy control group (group A), liposomal curcumin group (group B), PDGF-BB group (group C) and combined application group (group D). The rats in each group were divided into groups according to the experimental group. At the 4th and 8th weeks after operation, the general morphology, histomorphology, biomechanics and other aspects of rat tendon were detected and statistically analyzed. Results: The degree of tendon adhesion in group B and D was significantly less than that in group A and C, and the difference was statistically significant. The biomechanical test showed that the tendons in group C and D had higher strength and could withstand greater stress than those in group A and B. Conclusion: Liposome curcumin combined with PDGF-BB can significantly reduce the degree of adhesion after tendon injury, increase the strength of tendon healing, and significantly promote tendon healing.

Effects of Egg Size and Different Levels of Humidity during Incubation Period on the Embryonic Development, Hatching Percentage and Chicks Yield of Broiler Breeder

Background: This experiment was performed to investigate the effect of humidity and egg size on the hatchability, embryonic development and chicks yield in ROSS 308 broiler breeder flock. Methodology: The study was conducted in a completely randomized design with a 3×3 factorial arrangement. The treatments involved three levels of wet bulb humidity (°F WB) including 82, 82.5 and 83°F and three different egg sizes including small, medium or large. Results: The main effect of humidity level on the total and fertile hatchability (%) as well as the weight of one-day-old chicks were significant (P<0.05). Treatment with humidity of 82.5°F enhanced total and fertile hatchability, while humidity of 83°F increased one-day-old chick weight (P<0.05).A better total and fertile hatchability (%), fertility (%), chick yield (%) was observed in medium egg size group (P<0.05). However, the chick weight was greater in large egg size group (P<0.05). The lowest embryonic mortality was belong to medium egg size treatment (P<0.05). Conclusion: According to the results, it was concluded that humidity of 82.5°F as well as the medium egg size showed a better impact on the incubation performance.