Molecular analysis of the relationship between specific vaginal bacteria and bacterial vaginosis metronidazole therapy failure

The relationship between the intestinal and vaginal dysbiosis was explained. The necessity of correction of disbiotical changes with usage oral probiotics, in particular Laktovit Forte was demonstrated. The terms of prophylactical administration Laktovit Forte during pregnancy were described. Key words: microbiocenosis of the intestine and the vagina, bacterial vaginosis, pregnancy, probiotics, Laktovit Forte.

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The relationship between resolution of asymptomatic bacterial vaginosis and spontaneous preterm birth in fetal fibronectin positive women

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2004.10.258 ◽

2004 ◽

Vol 191 (6) ◽

pp. S105

Author(s):

Israel Hendler

Keyword(s):

Preterm Birth ◽

Bacterial Vaginosis ◽

Spontaneous Preterm Birth ◽

Fetal Fibronectin ◽

The Relationship

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Evaluation of Cytokines in Endocervical Secretion and Vaginal pH from Women with Bacterial Vaginosis or Human Papillomavirus

ISRN Obstetrics and Gynecology ◽

10.5402/2012/342075 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Ana Claudia Camargo Campos ◽

Eddie Fernando Candido Murta ◽

Márcia Antoniazi Michelin ◽

Cleomenes Reis

Keyword(s):

Immune Response ◽

Human Papillomavirus ◽

Bacterial Vaginosis ◽

Hpv Infection ◽

Control Group ◽

Microbiological Culture ◽

Vaginal Ph ◽

Cytokine Levels ◽

Th1 Cytokines ◽

The Relationship

Objective. To verify the relationship between vaginal pH and human papillomavirus (HPV) infection and to measure cytokine levels in endocervical secretions of women with bacterial vaginosis (BV) or HPV. Methods. 173 women (16–48 years old) were enrolled and divided into groups: BV, HPV, and controls. Microbiological culture and vaginal pH were measured. HPV detect by PCR, and cytokines by ELISA (IL-2, IL-6, IL-10, IL-12, TNF-α, and IFN-γ cytokines). Results. Of 173 women, 60 were control group (34.7%) and 113 were distributed in HPV (, 20.8%), BV (, 20.8%), vaginitis (, 17.3%) and, BV and HPV-associated groups (, 6.4%). Vaginal pH > 4.5 was related with HPV infection. IL-2 and IL-12 were increased in BV and HPV groups, and IL-6 (only BV group), compared to control group. IL-12 and IFN-γ were higher in HPV than BV group. Conclusion. The increase of vaginal pH was associated with HPV infection; BV and HPV groups had a Th1 cytokines immune response.

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Quantification and conceptual validation of the inoculum potential of Sclerotinia sclerotiorum in soybean and bean seeds

Journal of Seed Science ◽

10.1590/2317-1545v43236031 ◽

2021 ◽

Vol 43 ◽

Author(s):

Sueny Kelly Santos de França ◽

Carolina da Silva Siqueira ◽

Marina de Resende Faria Guimarães ◽

José da Cruz Machado

Keyword(s):

Seed Germination ◽

Real Time ◽

Molecular Analysis ◽

Sclerotinia Sclerotiorum ◽

Real Time Pcr ◽

White Mold ◽

Inoculum Potential ◽

Severe Damage ◽

The World ◽

The Relationship

Abstract: The fungus Sclerotinia sclerotiorum, the causal agent of white mold, is widespread throughout the world. The disease is considered to be one of the major diseases of soybean and bean crops in Brazil. The pathogen S. sclerotiorum is spread by soybean and bean seeds both in the form of sclerotia and dormant mycelium inside the seeds. The objective of this work was to evaluate the relationship between different potentials of S. sclerotiorum in soybean and bean seeds and the performance of these seeds, as well as to verify the localization and quantification of the inoculum of the pathogen in the seeds inoculated by Real-time PCR (qPCR), validating the term inoculum potential. Soybean and bean seeds were inoculated with the fungus by the osmotic conditioning method based on the exposure of the seeds to the fungus for periods of 24 h, 48 h, 72 h, and 96 h. Molecular analysis was carried out by means of qPCR in whole seeds and dissected in the integument, cotyledon and embryonic axis. The results showed that the effects of S. sclerotiorum on seed germination and vigor were progressive and proportional to the increases in inoculum potentials, since there was more severe damage to the seeds and consequently to the emerged plants at the highest potential (P96). The inoculum of the pathogen was found in all parts of the evaluated seeds, even at its lowest inoculum potential (P24), with an increasing DNA concentration, and the integument obtained a greater amount of DNA than the embryo, in comparison.

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Association of Bacterial Vaginosis and Human Papilloma Virus Infection With Cervical Squamous Intraepithelial Lesions

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqz021 ◽

2019 ◽

Vol 152 (2) ◽

pp. 185-189 ◽

Cited By ~ 2

Author(s):

Wissam Dahoud ◽

Claire W Michael ◽

Hamza Gokozan ◽

Amelia K Nakanishi ◽

Aparna Harbhajanka

Keyword(s):

Bacterial Vaginosis ◽

Human Papilloma Virus Infection ◽

Hpv Infection ◽

High Grade ◽

Squamous Intraepithelial Lesions ◽

Papilloma Virus ◽

Cervical Squamous Intraepithelial Lesions ◽

The Relationship ◽

Intraepithelial Lesions

ABSTRACT Objectives Human papillomavirus (HPV) is known to be associated with squamous intraepithelial lesions (SILs). However, there is limited and conflicting literature on the relationship between bacterial vaginosis (BV) and SIL. The aim of this study is to determine the prevalence of BV and evaluate the association between BV and SIL. Methods A retrospective study was performed on 10,546 cases between 2012 and 2017. HPV results were available in 7,081 cases. Results BV was present in 17.6% of cases. There was significant association between BV, positive HPV infection, and high-grade SIL. BV patients with negative HPV infection showed more squamous abnormalities than BV-negative HPV-negative patients. Conclusions We found there is a significant association between BV and SIL. BV is more common among patients with HPV infection and is independently associated with squamous abnormalities in cervical smears and surgical follow-up.

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Molecular analysis of HLA polymorphism clarifies the relationship between HLA disparity and outcome of bone marrow transplants

Human Immunology ◽

10.1016/0198-8859(92)90208-5 ◽

1992 ◽

Vol 34 (1) ◽

pp. 51

Author(s):

L.A. Baxter-Lowe ◽

J.T. Casper ◽

R.G. Hoffmann ◽

D.M. Dinauer ◽

M.J. Hessner ◽

...

Keyword(s):

Bone Marrow ◽

Molecular Analysis ◽

Bone Marrow Transplants ◽

Hla Polymorphism ◽

The Relationship

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The relationship between bacterial vaginosis and preterm birth. A review

Archives of Gynecology and Obstetrics ◽

10.1007/bf02505309 ◽

1997 ◽

Vol 259 (2) ◽

pp. 51-58 ◽

Cited By ~ 41

Author(s):

W. Chaim ◽

M. Mazor ◽

J. R. Leiberman

Keyword(s):

Preterm Birth ◽

Bacterial Vaginosis ◽

The Relationship

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The Relationship of Hydrogen Peroxide-Producing Lactobacilli to Bacterial Vaginosis and Genital Microflora in Pregnant Women

Obstetrics and Gynecology ◽

10.1097/00006250-199203000-00008 ◽

1992 ◽

Vol 79 (3) ◽

pp. 369-373 ◽

Cited By ~ 122

Author(s):

SHARON L. HILLIER ◽

MARIJANE A. KROHN ◽

SEYMOUR J. KLEBANOFF ◽

DAVID A. ESCHENBACH

Keyword(s):

Hydrogen Peroxide ◽

Bacterial Vaginosis ◽

Pregnant Women ◽

Relationship Of ◽

The Relationship

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Association between bacterial vaginosis and partner concurrency: a longitudinal study

Sexually Transmitted Infections ◽

10.1136/sextrans-2016-052652 ◽

2016 ◽

Vol 94 (1) ◽

pp. 75-77 ◽

Cited By ~ 9

Author(s):

Chris R Kenyon ◽

Jozefien Buyze ◽

Mark Klebanoff ◽

Rebecca M Brotman

Keyword(s):

Longitudinal Study ◽

Bacterial Vaginosis ◽

Mixed Effects ◽

Time Interval ◽

Vaginal Smears ◽

Nugent Score ◽

Time Period ◽

Partner Concurrency ◽

The Relationship ◽

Amsel Criteria

ObjectivesThe study aimed to test if there was an association between prevalent bacterial vaginosis (BV) and women reporting that their partner had other partners at the same time (partner concurrency). This association has not been assessed in a longitudinal cohort.MethodsThe Longitudinal Study of Vaginal Flora recruited a cohort of 3620 non-pregnant women aged 15–44 years who presented for routine primary healthcare at 12 clinics in Birmingham, Alabama. Behavioural questionnaires and vaginal smears were obtained quarterly for a year and BV was defined by a Nugent score 7 or higher as well as Amsel criteria. Mixed effects logistic regression was used to assess the relationship between prevalent BV and reporting that one's partner had other partners in the preceding 3–6 months time interval.ResultsNugent score prevalent BV was associated with both reporting that one's partner definitely (adjusted OR (aOR) 1.5; 95% CI 1.2 to 1.8) and possibly (aOR 1.5; 95% CI 1.2 to 1.8) engaged in partner concurrency in the preceding 3–6 months time period. Prevalent BV diagnosed by Amsel criteria was similar.ConclusionsA diagnosis of prevalent BV was associated with reporting that one's partner possibly or definitely engaged in partner concurrency.

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