scholarly journals Evaluation of Cytokines in Endocervical Secretion and Vaginal pH from Women with Bacterial Vaginosis or Human Papillomavirus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ana Claudia Camargo Campos ◽  
Eddie Fernando Candido Murta ◽  
Márcia Antoniazi Michelin ◽  
Cleomenes Reis
Keyword(s):  
Immune Response ◽  
Human Papillomavirus ◽  
Bacterial Vaginosis ◽  
Hpv Infection ◽  
Control Group ◽  
Microbiological Culture ◽  
Vaginal Ph ◽  
Cytokine Levels ◽  
Th1 Cytokines ◽  
The Relationship

Objective. To verify the relationship between vaginal pH and human papillomavirus (HPV) infection and to measure cytokine levels in endocervical secretions of women with bacterial vaginosis (BV) or HPV. Methods. 173 women (16–48 years old) were enrolled and divided into groups: BV, HPV, and controls. Microbiological culture and vaginal pH were measured. HPV detect by PCR, and cytokines by ELISA (IL-2, IL-6, IL-10, IL-12, TNF-α, and IFN-γ cytokines). Results. Of 173 women, 60 were control group (34.7%) and 113 were distributed in HPV (, 20.8%), BV (, 20.8%), vaginitis (, 17.3%) and, BV and HPV-associated groups (, 6.4%). Vaginal pH > 4.5 was related with HPV infection. IL-2 and IL-12 were increased in BV and HPV groups, and IL-6 (only BV group), compared to control group. IL-12 and IFN-γ were higher in HPV than BV group. Conclusion. The increase of vaginal pH was associated with HPV infection; BV and HPV groups had a Th1 cytokines immune response.

A combined ultrastructural and gene molecular research for the relationship between human uterine cervical carcinoma and human papillomavirus

1990 ◽  
Vol 48 (3) ◽  
pp. 204-205
Author(s):  
Kun Lee ◽  
Jingyi Si ◽  
Ricai Han ◽  
Wei Zhang ◽  
Bingbing Tan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Dot Blot ◽  
Homologous Sequences ◽  
Normal Cervical Epithelium ◽  
The Relationship ◽  
Chronic Cervicitis

There are more supports for the view that human papillomavirus (HPV) infection might be an etiological factor in the development of cervical cancer when the association of persistent condylomata is considered. Biopsies from 318 cases with squamous cell carcinoma of uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 normal cervical epithelium were collected from 5 geographic regions of China with different cervical cancer mortalities. All specimens were prepared for Dot blot, Southern blot and in situ DNA-DNA hybridizations by using HPV-11, 16, 18 DNA labelled with 32P and 3H as probes to detect viral homologous sequences in samples. Among them, 32 cases with cervical cancer, 27 with condyloma and 10 normal cervical epitheliums were randomly chosen for comparative EM observation. The results showed that: 1), 192 out of 318 (60.4%) cases of cervical cancer were positive for HPV-16 DNA probe (Table I)

scholarly journals Pro- and Anti-Inflammatory Cytokines in the First Trimester—Comparison of Missed Miscarriage and Normal Pregnancy

2021 ◽  
Vol 18 (16) ◽  
pp. 8538
Author(s):  
Maciej Kwiatek ◽  
Tomasz Gęca ◽  
Anna Kwaśniewska
Keyword(s):  
Immune Response ◽  
Inflammatory Cytokines ◽  
First Trimester ◽  
Normal Pregnancy ◽  
Control Group ◽  
Study Group ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Tgf Β1

The advantage in response of Th2 over Th1 is observed in normal pregnancy in peripheral blood. A disturbance of this balance can lead to symptoms of miscarriage and pregnancy loss. The aim of this study was to evaluate the pro- and anti-inflammatory cytokines in sera of women who were diagnosed with missed miscarriage in the first trimester and to compare this systemic immune response to the response in women with normal pregnancy. The study group consisted of 61 patients diagnosed with missed miscarriage. In total, 19 healthy women with uncomplicated first trimester created the control group. Cytokines were determined in the maternal serum by ELISA. The analysis included INF-γ, TNF-α, Il-1β, Il-4, Il-5, Il-6, Il-9, Il-10, Il-13 and TGF-β1. Th1 cytokine levels in the study group reached slightly higher values for INF-γ, Il-1β and slightly lower for IL-6 and TNF-α. In turn, Th2 cytokine levels in the study group were slightly higher (Il-9, Il-13), significantly higher (Il4, p = 0.015; Il-5, p = 0.0003) or showed no differences with the control group (Il-10). Slightly lower concentration involved only TGF-β1. Analysis of the correlation between levels of pro- and anti-inflammatory cytokines resulted in some discrepancies, without showing predominance of a specific immune response. The results did not confirm that women with missed miscarriage had an advantage in any type of immune response in comparison to women with normal pregnancy.

scholarly journals The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

2021 ◽  
Author(s):  
Shuang Zhang ◽  
Hui-Min Wu ◽  
Xiang-Ni Cao ◽  
Xian-Qi Zhang ◽  
Gui-ping Gao
Keyword(s):  
Tear Film ◽  
Fungal Keratitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Controls ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Healthy Control ◽  
The Relationship ◽  
Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

Distribution of Lactobacillus species in Iranian women with both human papillomavirus (HPV) infection and bacterial vaginosis (BV)

Meta Gene ◽  
2020 ◽  
Vol 26 ◽  
pp. 100791
Author(s):  
Roghayeh Ghaniabadi ◽  
Shirin Hashemi ◽  
Morteza Soltanian Bajgiran ◽  
Sahar Javadi ◽  
Nima Mohammadzadeh ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Bacterial Vaginosis ◽  
Hpv Infection ◽  
Lactobacillus Species ◽  
Iranian Women

scholarly journals Effect of paiteling on the alteration of persistent HR-HPV infection after panhysterectomy

2018 ◽  
Vol 16 ◽  
pp. 205873921881917
Author(s):  
Ye Zhao ◽  
Qiu-Man Wang ◽  
Li-Ting Yao ◽  
Xiao Han
Keyword(s):  
Human Papillomavirus ◽  
Adverse Reactions ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Control Group ◽  
The Third ◽  
Conversion Rates ◽  
The Difference ◽  
And Control ◽  
Negative Conversion

This study aims to determine the effect of paiteling by placing a vaginal stump on the outcome of persistent high-risk human papillomavirus (HR-HPV) infection after hysterectomy for cervical intraepithelial neoplasia (CIN). From January 2013 to December 2015, 125 patients were enrolled in the study. These patients were divided into groups, according to patient’s preference. There were 55 patients in the paiteling group, while there were 70 patients in the control group. At the end of the third and ninth month, Thinprep cytology test (TCT) and human papillomavirus (HPV) typing were reviewed to determine the changes in HR-HPV and its complications. The negative conversion rates of these two groups were observed. Patients in the control group were observed and followed up without treatment. At the third month after treatment, HPV negative rates were 83.64% and 34.29% in the paiteling group and control group, respectively (χ2 = 30.444, P < 0.01). In the ninth month, the rate of HPV negative conversion was 90.91% in the paiteling group and 48.57% in the control group (χ2 = 25.047, P < 0.01). The difference between the two groups was statistically significant, and patients in both groups had no obvious adverse reactions. Paiteling irrigation at the vaginal stump can accelerate the positive-to-negative conversion of HR-HPV infection after panhysterectomy.

scholarly journals Human Papillomavirus and Genital Disease in Men: What We Have Learned from the HIM Study

2019 ◽  
Vol 63 (2) ◽  
pp. 109-117 ◽  
Author(s):  
Laura Sichero ◽  
Anna R. Giuliano ◽  
Luisa Lina Villa
Keyword(s):  
Human Papillomavirus ◽  
Natural History ◽  
The United States ◽  
Hpv Infection ◽  
Clinical Consequences ◽  
History Of ◽  
Crucial Information ◽  
Associated Risk Factors ◽  
The Relationship ◽  
Genital Disease

It is currently recognized that in addition to the major impact of human papillomavirus (HPV) infection in females, HPV causes considerable disease in men at the genitals, anal canal, and oropharynx. Specifically, genital HPV infections may progress to genital warts and penile carcinoma. Although studies concerning the natural history of HPV infections and associated neoplasias have mainly focused on women, during the last 2 decades considerable attention has been given in further understanding these infections in men. The HIM (HPV infection in men) Study, the only prospective multicenter study of male HPV natural history, consisted of a large prospective international cohort study in which men from Brazil, the United States, and Mexico were enrolled. The design and protocols of this study allowed unraveling crucial information regarding the relationship between HPV infection and clinical consequences in men, and associated risk factors at each of the anatomic sites where HPV is known to cause cancer in men.

scholarly journals Human papillomavirus as a single infection in pregnant women from Northeastern Mexico: Cross-sectional study

2020 ◽  
Author(s):  
Bernardo Martínez-Leal ◽  
Karla Ivette Álvarez-Banderas1 ◽  
Homero Sánchez-Dávila ◽  
Martha Imelda Dávila-Rodríguez ◽  
Elva Irene Cortés-Gutiérrez
Keyword(s):  
Human Papillomavirus ◽  
Pregnant Women ◽  
Hpv Infection ◽  
Smoking History ◽  
Single Infection ◽  
Multiple Infections ◽  
Control Group ◽  
Cervical Lesions ◽  
Cross Sectional ◽  
Northeastern Mexico

Background: The role of human papillomavirus (HPV) as single or multiple infections in pregnant women would be relevant to determining the time to progression and/or the time to regression of cervical lesions. Objective: In this preliminary study, we determined the prevalence of HPV as single or multiple infections in pregnant women from Northeastern Mexico. Materials and Methods: Samples from 31 pregnant and 62 nonpregnant women were examined between January 2015 and November 2015 at UMAE-23 of the Instituto Mexicano del Seguro Social (IMSS). The samples of cervicovaginal exudate were obtained for HPV DNA detection using the INNO-LiPA test, and HPV infections were analyzed as single or multiple infections. Participants completed a questionnaire on sociodemographic, gynecological, obstetric, and sexual behavior characteristics. Results: The mean age of the pregnant women was 25.7 ± 4.8 yr, with an average time of pregnancy of 6 ± 1 months at the time of the study. With respect to age, parity, smoking history, or oral contraceptive use no statistically significant differences between the two studied groups was observed. The HPV infection was 2.7 times higher in pregnant women (35%) than in the control group (13%). In total, 78% of the pregnant women who were HPV-positive presented with single infections compared with 28% of the nonpregnant women. Conclusion: A higher prevalence of HPV as a single infection was found in this sample of pregnant Mexican women. Follow-up is necessary to evaluate the persistence or regression of the infection. Key words: Papillomavirus infection, Pregnant women, Uterine cervical dysplasia.

scholarly journals Importance of High-Risk Human Papillomavirus Infection Detection in Female Renal Transplant Recipients in the First Year after Transplantation

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Maksims Cistjakovs ◽  
Alina Sultanova ◽  
Olga Jermakova ◽  
Liba Sokolovska ◽  
Svetlana Chapenko ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune System ◽  
Renal Transplant ◽  
Immunosuppressive Therapy ◽  
Hpv Infection ◽  
Control Group ◽  
First Year ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
One Year

Objectives. Most of human papillomavirus (HPV) infections are “cleared” by the immune system; however, in cases of immune system suppression, infections could lead to development of malignancies. The aim of this study was to find out the frequency of HR-HPV infection in early period after renal transplantation in recipients receiving immunosuppressive therapy and to follow the progression of the infection up to one year. Methods. 43 female renal transplant recipients and 79 healthy female individuals as a control group were enrolled in this investigation. For the detection of HPV infection, patients’ samples (blood and vaginal swabs) were collected two weeks after transplantation with following collection of six months and one year. Different polymerase chain reactions for HR-HPV genomic sequences detection and ELISA kit for detection of anti-HPV IgG antibodies were used. Results. In this study, we show that frequency rate of HR-HPV infection has increased in the first year after transplantation from early stage of immunosuppressive therapy (from 24% to 36%). Also an increase of HR-HPV load was detected over time, showing the highest median viral load at sixth month after transplantation. Conclusions. From the obtained data, it follows that it is very important to carefully monitor patients receiving immunosuppression therapy on progression of HR-HPV.

Association of Bacterial Vaginosis and Human Papilloma Virus Infection With Cervical Squamous Intraepithelial Lesions

2019 ◽  
Vol 152 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Wissam Dahoud ◽  
Claire W Michael ◽  
Hamza Gokozan ◽  
Amelia K Nakanishi ◽  
Aparna Harbhajanka
Keyword(s):  
Bacterial Vaginosis ◽  
Human Papilloma Virus Infection ◽  
Hpv Infection ◽  
High Grade ◽  
Squamous Intraepithelial Lesions ◽  
Papilloma Virus ◽  
Cervical Squamous Intraepithelial Lesions ◽  
The Relationship ◽  
Intraepithelial Lesions

ABSTRACT Objectives Human papillomavirus (HPV) is known to be associated with squamous intraepithelial lesions (SILs). However, there is limited and conflicting literature on the relationship between bacterial vaginosis (BV) and SIL. The aim of this study is to determine the prevalence of BV and evaluate the association between BV and SIL. Methods A retrospective study was performed on 10,546 cases between 2012 and 2017. HPV results were available in 7,081 cases. Results BV was present in 17.6% of cases. There was significant association between BV, positive HPV infection, and high-grade SIL. BV patients with negative HPV infection showed more squamous abnormalities than BV-negative HPV-negative patients. Conclusions We found there is a significant association between BV and SIL. BV is more common among patients with HPV infection and is independently associated with squamous abnormalities in cervical smears and surgical follow-up.

The effects of an educational program based on the Theory of Reasoned Action on high-risk sexual behaviors among patients with human papillomavirus infection: a randomized controlled trial

2020 ◽  
Author(s):  
Nima Pourgholamamiji ◽  
Hooman Shahsavari ◽  
Arpi Manookian ◽  
Tahereh Soori ◽  
Maryam zandkarimkhani
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Sexual Behaviors ◽  
Educational Program ◽  
Theory Of Reasoned Action ◽  
Reasoned Action ◽  
Controlled Trial ◽  
Intervention Group ◽  
Hpv Infection ◽  
Control Group

Abstract Background: Sexually transmitted diseases are a major health problem in all countries. Human papillomavirus (HPV) infection is the most common viral sexually transmitted disease in both genders. High-risk sexual behaviors (HRSBs) are among the major risk factors for HPV infection. Lack of knowledge can contribute to HRSBs and hence, education can potentially reduce HRSB risk. This study evaluated the effects of an educational program based on the Theory of Reasoned Action on HRSBs among patients with HPV. Methods: This two-group randomized controlled trial was conducted in 2017-2018. The educational program was developed based on the results of a survey into the most important components of the Theory of Reasoned Action contributing to HRSBs among 100 patients recruited from Razi hospital, Tehran, Iran. Then, 110 patients from the same hospital were consecutively recruited and randomly allocated to a control (n = 55) and an intervention (n = 55) group and all of them completed a demographic questionnaire and the Sexual Risk Scale. Then, each participant in the intervention group was provided with the developed educational program which consisted of two face-to-face educational sessions in the first and the thirtieth days of the intervention, an educational booklet, and biweekly follow-up telephone contacts. Their counterparts in the control group solely received routine care services. Three months after the first educational session, HRSBs were reassessed in both groups. Results: There were no significant between-group differences respecting participants’ baseline characteristics and mean score of HRSBs (P > 0.05). After the intervention, the mean score of HRSBs significantly decreased in the intervention group (P < 0.001) but did not significantly change in the control group (P = 0.70). The posttest mean score of HRSBs in the intervention group was significantly less than the control group (P = 0.015). Conclusion: Education based on the Theory of Reasoned Action is effective in significantly reducing HRSBs among patients with HPV infection. Nurses can develop and use educational interventions based on this theory to reduce HRSBs and HPV infection. Trial registration: IRCT2016121831453N1 (8 Oct 2017) Keywords: Theory of Reasoned Action, High-risk sexual behaviors, Human papillomavirus

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