Kinematic study of root elongation in Arabidopsis thaliana with a novel image-analysis program

2012 ◽  
Vol 126 (1) ◽  
pp. 187-192 ◽  
Author(s):  
Akitoshi Iwamoto ◽  
Eri Kondo ◽  
Hirotomo Fujihashi ◽  
Munetaka Sugiyama
Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 249
Author(s):  
Weimiao Liu ◽  
Liai Xu ◽  
Hui Lin ◽  
Jiashu Cao

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.


1988 ◽  
Vol 34 (1) ◽  
pp. 29-34 ◽  
Author(s):  
P. Hufnagl ◽  
G. Wolf ◽  
K. Wenzelides ◽  
H. Martin ◽  
H. Guski ◽  
...  

2014 ◽  
Vol 70 (3) ◽  
pp. 187-198
Author(s):  
Ewa Kupidłowska

The ultrastructure and morphology of roots treated with coumarin and umbelliferone as well as the reversibility of the coumarins effects caused by exogenous GA, were studied in <em>Arabidopsis thaliana</em>. Both coumarins suppressed root elongation and appreciably stimulated radial expansion of epidermal and cortical cells in the upper part of the meristem and in the elongation zone. The gibberellic acid applied simultaneously with coumarins decreased their inhibitory effect on root elongation and reduced cells swelling.Microscopic observation showed intensive vacuolization of cells and abnormalities in the structure of the Golgi stacks and the nuclear envelope. The detection of active acid phosphatase in the cytosol of swollen cells indicated increased membrane permeability. Significant abnormalities of newly formed cell walls, e.g. the discontinuity of cellulose layer, uncorrect position of walls and the lack of their bonds with the mother cell wall suggest that coumarins affected the cytoskeleton.


2008 ◽  
Vol 591-593 ◽  
pp. 611-615
Author(s):  
Adriana Scoton Antonio Chinelatto ◽  
Milena K. Manosso ◽  
Elíria Maria Jesus Agnolon Pallone ◽  
Adilson Luiz Chinelatto

The control of the heating curve to manipulate microstructure during sintering is a way that has being studied and it presents advantages such as simplicity and economy. In this work, it was studied the sintering in two-steps of a commercial ultrafine alumina. For this, the alumina power was deagglomerated in milling ball and the specimens for sintering were pressed. Sintering was performed in a dilatometer, with constant heating rate of 15°C/min up to 1500°C. By these results, heat treatment temperatures for two-step sintering were defined. The sintering specimens were characterized through the apparent density measures using Archimedes method, the grain size measures using image analysis program and microstructural analysis using a scanning electron microscope. The results showed that the two-step sintering influence in the development of the final microstructure and permit the control of the grain size and density.


2003 ◽  
Author(s):  
Abhinav Saxena ◽  
Jordan L. Dorrity ◽  
Youjiang Wang

One area of research at Georgia Tech recently has focused on the measurement of Card-web density. This research is part of a study to spin yarns directly from a card web thus eliminating several processes. A Card-Spinning system has been designed which can quantify the cross-machine and machine direction web non-uniformity using a non-contact web density measurement technique. The web monitoring system for Card-Spinning uses a line scan camera to capture the image continuously and is equipped with an image analysis program, to characterize the web quantitatively, predicting the product quality. A multifunctional graphical interface has been designed for the image analysis program, which can be used to calculate the statistics for the non-uniformity of the card web both online and offline. This would help in characterizing the nature of non-uniformity and to understand the system better before employing methods to improve it. This paper discusses the development and results from the work.


1999 ◽  
Vol 37 (9) ◽  
pp. 671-678 ◽  
Author(s):  
Dario Leister ◽  
Claudio Varotto ◽  
Paolo Pesaresi ◽  
Alexandra Niwergall ◽  
Francesco Salamini

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